Food and drinking water are the main sources of trace and major elements. Besides the elements that are vitally essential for living organisms and human health, food may contain the traces of potentially toxic elements. Environmental site specific impact is one of the influencing factors of elemental content in plants that is important issue also for quality of food crops. Quantitative content of several trace elements (e.g., Al, As, Cd, Co, Cr, Cu, Ni, Pb, Zn) detected in food crops reveal environmental background levels as well as it can be associated with unexpected food contamination. Current study involves quantitative analysis of more than 200 root vegetable samples (onions, carrots and potatoes) grown and collected in Latvia in the harvesting season of 2010. Within this study the quantitative analysis of vegetables for 9 elements (As, Cd, Co, Cr, Cu, Ni, Pb, Se, Zn) was performed. After the wet digestion of samples, the quantitative analysis was done by using inductively coupled plasma mass spectrometry (ICP-MS). Obtained results revealed wide ranges of trace elements in root vegetables, including potentially toxic elements. Comparison of element content in edible parts of vegetables and potato peel showed that a great part of elements (e.g., As, Co, Cr, Pb) is concentrated in peel; however, some elements (e.g., Cd, Se, Zn) are taken up by plants, and therefore may contaminate food more easily. Element transfer routes and their biochemistry is a complicated issue that is affected by natural environmental factors as well as by anthropogenic activities.

Nutritional value and shelf life of milk and dairy products depend on the composition and stability of their constituents. The aim of the present study was to evaluate the effects of carrots as cow feed carotenoid source on butter oil fatty acid (FA) composition and oxidative stability. Milk was obtained from one trial group (TG; n=5) and one control cow group (CG; n=5) in a conventional dairy farm in Latvia. TG cows received carrots 7 kg per cow per day; the length of the supplementation period was 39 days. The stability of butter oil exposed to sunlight (3h) and held for 14 days in the temperature of 60 °C was analyzed by peroxide value method. The changes of the FA content and ratios in TG milk fat were more positive with respect to the fat nutritional value as observed in CG – stronger increase in the content of polyunsaturated FA (p is less than 0.05), and in the ratio between stearic and palmitic acids (p is less than 0.05); also a tendency was seen of increasing content of short and monounsaturated FA, as well as decreasing the ratio between ω6 and ω3 FA groups. Oxidative stability of the carrot supplemented cow group’s milk butter oil samples that were collected after 25- day trial period, exposed to sunlight (3h) and stored at a temperature of 60 °C was significantly (p is less than 0.05) higher compared to CG samples. After the 39-day long trial period stability difference was not significant, despite the tendency that average polyunsaturated FA content in TG samples was higher compared to the control.

Carrots (Daucus carota L.) are a globally important vegetable crop providing a source of important nutritional compounds through their carotenoid content whilst adding flavour and texture to many diets across the world. The current research focuses on the evaluation of sugars, vitamin C and dietary fibre content in ‘Nante’ hybrid carrots. The research was accomplished on fresh in Latvia cultivated carrots harvested in Zemgale region in the first part of October 2011 and immediately used for experiments. Late-bearing hybrids of ‘Nante’ carrots were used for analysis: Nante/ Berlikum, Nante/Maestro, Nante/Forto, Nante/Bolero, and Nante/Champion. The major sugars (fructose, glucose and sucrose) were determined by applying the method of high performance liquid chromatography, vitamin C - by titration with 0.05 M iodine solution, and dietary fibre - using standard method No 985.29. In the present experiments it was found that there are significant differences in the sugar, vitamin C and dietary fibre content between different carrot hybrids. The highest content of total sugars was found in Nante/Maestro and Nante/Champion 7.99 g 100 gE-1 and 7.57 g 100 gE-1 hybrids in fresh weight, respectively. The lowest total sugars content was in Nante/Berlikum hybrid − 4.03 g 100 gE-1. Vitamin C content in carrot hybrid Nante/Maestro was the highest 17.61±0.17 mg 100 gE-1, but in hybrid Nante/Champion - the lowest 8.39±0.17 mg 100 gE-1 of fresh weight. The dietary fibre content in analysed carrot samples ranged from 34.25±0.47 g 100 gE-1 in Nante/Maestro to 25.78±1.54 g 100 gE-1 in Nante/Champion hybrids.

The main purpose of the present experiments was to investigate the effect of various hydrogen peroxide (H2O2) concentrations and for various lengths of treatment on the total carotenoid, β–carotene content, colour intensity and microbiological safety on the fresh shredded carrots. Shredded carrots were dipped in 0.5, 1.0 and 1.5% H2 O2 water solution for 30 ± 1s, 60 ± 1s and 90 ± 1s. Negative effect of H2 O2 on β–carotene content and colour parameters of analyzed shredded carrots samples was not detected. In carrots treated with H2 O2 (p = 0.008) for 60 – 90s the total content of carotenoids significantly decreased during treatment compared to untreated carrot samples. There was significant difference (p is less than 0.05) observed between treated and non–treated shredded carrot samples on the total bacteria count. It was possible to reduce significantly (p is less than 0.05) the content of yeasts and mould up to 99.99% by shredded carrots treatment with 1.5% hydrogen peroxide water solution for 30 ± 1s. In the non–processed carrots E.coli was detected; however, it was possible to destroy E.coli by treating carrots with 0.5% H2 O2 water solution for 30 ± 1s. Considering all experimentally obtained results, we have concluded that fresh shredded carrots could be treated in water with the addition of hydrogen peroxide 1.5% for 30 ± 1s to maintain quality.

Fruits and vegetables are a major source of antioxidants. The aim of current research was to study and compare the antiradical activity, the total polyphenol content (TPC) and the total flavonoid content (TFC) in dried carrots (Daucus carota), pumpkins (Cucurbita maxima), leeks (Allium ampeloprasum var. porrum) and black radish (Raphamussativus) using a traditional convective drier and a microwave-vacuum drier. For each vegetable steaming as pre-treatment was used. Vegetables were harvested in Latvia in 2012, gathered when ripe and then dried. Analyses were made in Latvia University of Agriculture, Faculty of Food Technology laboratories. The total polyphenol content was determined by the Folin- Ciocalteu method and the total flavonoid content - using spectrophotometric method. The antiradical activity was analyzed by the 2,2-diphenyl-1-picrilhydrazyl (DPPH) assay. The results of experiments demonstrate that the total amount of polyphenols ranged from 98.97 to 623.70 mg gallic acid equivalent (GAE) 100 gE-1 in dry weight and the total amount of flavonoids ranged from 40.32 to 100.23 mg catechin equivalent (CE) 100 gE-1 in dry weight. The value of DPPH antiradical activity for vegetable samples ranged from 6.10 to 45.14 percents.

The quality and nutritional value of milk and dairy products are considerably influenced by the stability of its constituents. The aim of the present study was to evaluate the possibility of enhancing oxidative stability of cow’s milk fat and vitamin B2 using carrots and palm oil feed supplement CAF 100 as sources of natural antioxidants in cow feed. Milk samples were collected after 25-day period of feed supplementation. The intensity of riboflavin losses during its photo oxidative degradation in sunlight was measured by the fluorometric method. A slight and significant (p is less than 0.05) difference in higher vitamin B2 stability was seen in carrot-supplemented group (TG1) milk, where the losses of vitamin B2 were by 3.01% less compared to CG (CG). The oxidative stability of butter oil samples stored in 60 °C temperature was analyzed by peroxide value (PV) method. The oxidative stability of samples initially affected by light from both trial groups was significantly (p is less than 0.05) higher compared to CG showing the good potential of the cow diet enrichment with carotenoid additives. The longest induction period (larger than 14 days) was observed in CAF-100- supplemented (TG2) cow milk fats, which can be explained by carotenoids and tocopherol presence and its possible synergism in fat protection. The induction periods of the TG1 and CG were 12.03 and 10.97 days, respectively.

β-Carotene (BC) and a-tocopherol in milk fat have positive implications in human nutrition, besides the specifically protecting polyunsaturated fatty acids from oxidation. To determine the possible effect on some milk components, nutritional value and lipid stability of including carrots in the diet of lactating cows, ten cows were selected in a dairy farm and divided into 2 groups (control and experimental) by 5 cows in each. In experimental group's diet additionally 7 kg carrots per cow per day were included. Individual cow milk samples were obtained 1 day before feed enrichment, in days 7, 24, 35, 42 (during the feed enrichment), and 1 week after feed enrichment. Analyses of milk fat, protein content, somatic cell count (SCC), retinol, BC, vitamin C, tocopherols, immunoglobulins (Ig), lysozyme, fatty acids (FA) were made, and milk yield was measured. Supplying cow diet with carrots showed a tendency to improve milk quality by promoting the faster decrease of SCC, and significantly (p is less than 0.05) increasing Ig and lysozyme content, thus potentially improving milk nutritional value. The vitamin content rose significantly (p is less than 0.05) by retinol and tocopherol contents, but b-carotene content increase in milk was not observed. Also milk fatty acid (FA) stability changes during 5 day storage in temperature of 4 - 6 deg C were not observed.

Vegetable processing in food industry results in significant number of by-products – peel, mark, bark, seeds still rich in bioactive compounds. The objective of this study was to investigate the stability of total phenolic content (TPC) and antioxidant activity after high temperature short time (HTST) extrusion cooking of a rye and oat- based matrix with addition of apple, carrot and pumpkin by-product flour (BPF) in various amounts (5%, 10%, 15% and 20%). Extrusion was performed with co-rotating twin-screw extruder (compression ratio 8:1) at Ltd MILZU. The main drive of extruder was provided with a 7.5 HP motor (400 V, 3 HP, 50 cycles). Temperatures for extrusion zones were 125 °C /135 °C /145 °C. TPC was determined using the Folin Ciocalteu method. Antioxidant activity was evaluated by free radical 2, 2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant scavenging activity. TPC in cereal-based crispbread was 62.03 ± 0.15 mg GAE gE-1 DW before extrusion and 37.73 ± 1.96 mg GAE gE-1 DW after extrusion. Addition 20% of apple BPF increased TPC in crispbread to 193.92 ± 1.37, carrot BPF 171.36 ± 6.97 and pumpkin BPF to 195.09 ± 4.68 mg GAE gE-1 DW after extrusion. Antioxidant activity of control (20% oats, 80% rye flour blends) sample was 0.516 ± 0.192 mg TE gE-1 DW but in samples with addition of 20% by-products, it was significantly higher reaching 0.926 ± 0.05 TE gE-1 DW in samples with pumpkin by-products after extrusion.

The aim of the research was to evaluate how the quality of carrots is affected by organic and conventional production systems. The experiment was carried out at the Estonian Crop Research Institute in 2009. Conventional treatment received the following amount of nutrients via mineral fertilizers: N 115, P 40 and K 152 kg haE-1. For plant protection, the following pesticides were used: Fenix, Fastac 50, Agil and Signum. In organic cultivation system compost and humic acid solution Humistar were used for fertilization and polypropylene non-woven fabric Agryl P-17 for plant protection. Marketable yield of organic carrots was 8% lower compared to the conventional carrots. Conventional carrots contained pesticide residues and had significantly higher nitrate concentration than organic carrots. The contents of total sugars, phosphorus, potassium, calcium and magnesium did not differ significantly between carrots from different cultivation systems. At harvest, dry matter (DM) content of organically grown carrots was significantly higher, whereas vitamin C and ß-carotene content was significantly lower in organically grown carrots. However, after 5-months of storage, the organic carrots had significantly higher total soluble solids (TSS) and β-carotene content compared to the conventional ones indicating that organically grown carrots were less susceptible to storage conditions.