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Serum progesterone, 17-hydroxyprogesterone and 17 B-oestradiol during the oestrous cycle in the swamp buffalo in Thailand
1981
Prasit Bodhipaksha
A radioimmunoassay for measuring serum progesterone (P), 17-hydroxyprogesterone (17-OHP) and 17-B-oestradio (E2) in buffaloes was described. One cm3 of pooled serum with three steroids added for recovery was extracted with ether and chromatographed on a celite microcolumn. The recovery of the standard P, 17-OHP and E2 was 72.9-89.6%, 77.0-97.0% and 85.5-101.3% respectively. The precision of within assay variance of these three steroids expressed as percentage of coefficient of variations was 8.2% (P and 17-OHP) and 8.3% (E2). The between assay variance was 13.2%(PT), 11.2% (17-OHP) and 12.9%(E2). The sensitivity of the assay varied between 3.5-25.0 pg. P, 4.0-25.0 pg 17-OHP and 2.5-10.0 g E2. Levels of serum progesterone, 17-hydroxyprogesterone and 17-B oestradiol were measured in the same serum samples at daily stages of the oestrous cycle in four female buffaloes; altogether there were 11 cycles. The swamp buffaloes have the normal oestrous cycles of 19-27 days. Progesterone levels in pre-oestrous-oestrous were 9.7 +-8.5 ng/100 cm3, the level beginning to rise by day 7 or 10, and reaching a peak of 38.8 +-12.2 ng/100 cm3 in 15.6 +-3.7 days of the cycle, then declining rapidly to the basal level about 4-7 days before the next oestrous cycle. The serum levels of 17-hydroxyprogesterone rose significantly in three peaks: preostrous-oestrous (21.3 +-8.3 ng/100 cm3), metoestrous (14.6+-6.6 ng/100 cm3) and dioestrous (20.2 +-8.1 ng/100 cm3). The average values in these three phases were 15.1+-7.1 ng/100 cm3, 9.7+-5.2 ng/100 cm3 and 10.6+-4.0 ng/100 cm3 respectively. The basal levels were below 5.0 ng/100 cm3. The oestradiol levels reached a peak on the day before oestrous. The average value of the peaks was 13.0+-4.0 ng/100 cm3. The duration of the peak was about four days, then it sharply declined to basal levels of 3.9+-1.0 ng/100 cm3.
显示更多 [+] 显示较少 [-]Revitalized agriculture for balanced growth and resilient livelihoods: Toward a rural development strategy for Mon State, Myanmar 全文
2018
International Food Policy Research Institute | Michigan State University | Center for Economic and Social Development | Filipski, Mateusz J. | Nischan, Ulrike | van Asselt, Joanna | Holtemeyer, Brian | Schmidt, Emily | Kedir Jemal, Mekamu | Kennedy, Adam | Zhang, Xiaobo | Dorosh, Paul A.
Revitalized agriculture for balanced growth and resilient livelihoods: Toward a rural development strategy for Mon State, Myanmar 全文
2018
International Food Policy Research Institute | Michigan State University | Center for Economic and Social Development | Filipski, Mateusz J. | Nischan, Ulrike | van Asselt, Joanna | Holtemeyer, Brian | Schmidt, Emily | Kedir Jemal, Mekamu | Kennedy, Adam | Zhang, Xiaobo | Dorosh, Paul A.
Building a global information network for agricultural and rural research: The SINGER system
1998
Perry, M. C. | O'Nolan, P.
Studies of semen of the Thai swamp buffalo
1981
Prasit Bodhipaksha | Peerasak Chantaraprateep
The suitable diluters for the buffalo semen are milk yolk and coconut milk yolk. Diluted semen is kept at 4-5 degrees Centigrade with the result of sperm survice 6-2 days, in some cases the viable sperm up to 60 percent for 8 days in recorded.
显示更多 [+] 显示较少 [-]Symptoms of heat and conception rate of swamp buffaloes after estrus induction with prostaglandin F2 alfa
1981
Prachin Virakul
Caesarean section in swamp buffalo
1981
Peerasak Chantaraprateep
Immuno response of clariid catfish (Clarias batrachus) to Aeromonas hydrophila vaccine
1981
Kriengsag Saitanu | Kriengsak Poonsuk
Innovations in rural microfinance for the rural poor: exchange of knowledge and implications for policy
2000
Sharma, Manohar | Zeller, Manfred
Unlocking markets and trade for rural development
2015
International Food Policy Research Institute
The complexity of supply and demand and the nuances of trade and markets have grown exponentially as the world grows smaller. Fortunately, so have our tools to understand them and to harness the knowledge for pro-poor benefits.
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