Two recombinant influenza A virus vectors expressing the ESAT 6 and TB10.4 mycobacterial proteins from the non-structural (NS) gene were constructed via reverse genetics technique to develop a specific means of prophylaxis for bovine tuberculosis. We experimented to determine optimal conditions for growing recombinant vectors in Vero cell culture and chick embryos. This study established that the maximum amount of virus builds up in a Vero cell culture with the Dulbecco’s Modified Eagle’s Medium (DMEM) serum-free medium. However, using cell culture to produce vector vaccines is labour-intensive and inefficient. An alternative way, a traditional, time-tested technique, is provided by growing samples in chick embryos. One of the advantages of this technique is its affordability and availability, enabling easy scale-up of vaccine production. In the optimization experiments, the FLU-ΔNS_ESAT 6 and FLU-ΔNS_ТВ10.4 viruses constructed were inoculated into 10-day-old chick embryos. It was determined that the optimal incubation temperature that led to the highest virus build-up was 37 ± 0.5 °С. And the infectious activity level of the FLU-ΔNS_ESAT 6 recombinant vector was at 8.95 ± 0.07 log10EID50 0.2 cmE−3, while that of the FLU-ΔNS_ТВ10.4 was at 9.20 ± 0.07 log10EID50 0.2 cmE−3, what was provided by infectious doses of 1000–10000 EID50 , which makes it possible to create a virus-containing material with a hemagglutination activity level of 1:64. The size of recombinant vector amplicons expressing proteins ТВ10.4 and ESAT 6 was 1170 bp and 1175 bp, respectively. Electron microscopy images confirm that the developed virions are morphologically similar to the avian influenza virus.

Not only does construction activity in the regions play a crucial role in the regional development, as it not only provides employment opportunities and thus contributes to the well-being of the population, but also creates favourable conditions for access to modern housing and requires the mobility of human resources to the regions. At the same time, construction in regions and rural areas often implies more difficult conditions than in urban environments, which are associated with both funding opportunities and working conditions. The availability of financial investments to the private sector is limited for the construction of housing outside Riga and Pierīga region, which significantly reduces construction opportunities in the regions of Latvia. The study evaluates the possibilities of obtaining new housing in the regions of Latvia, analyses the trends in construction volume and costs from 2015, modelling forecasts until 2026. The study looks at various economic instruments that could contribute to the development of construction and new housing in the regions. The study uses surveys of municipalities, entrepreneurs and experts in the field of construction, in-depth interviews, focus groups and statistical data processing methods. It has been concluded that Latvian entrepreneurs tend to postpone investments or invest only in the economically strongest moments, which further aggravates the gap between urban and rural areas. The trends identified in the study indicate a possible further direction of stagnation or recession in the construction sector of new housing in the regions of Latvia, which will directly affect regional development in the next five years. The study highlights the necessary interventions for more productive regional housing stock cohesion.

Social benefits are essential in providing support to families with children, especially in the first years of the child’s life as well as to families at risk of poverty (large families, parents raising a child alone). Social insurance benefits ensure parents with income while taking care of a child. Latvia’s strategic planning documents have defined social protection for families with children as one of the priority areas for several planning periods simultaneously emphasising the need to increase social security and promote the birth rate in the country. The research aim is to assess the social benefits system for families with children in Latvia. The research results allow concluding that the expenditure for social support for families with children as percentage of GDP in Latvia is lower than the average EU Member States indicator as well as it is the lowest one among the Baltic States. Support for families with children in Latvia is gradually growing; however, it is necessary to regularly increase the childbirth allowance taking into account inflation in order not to worsen the financial situation of these families as well as to allocate a larger proportion of GDP to support families with children in Latvia.

Late-life suicide is a significant public health concern that has been associated with a range of social and individual factors. Social factors, such as social isolation, lack of social support, and financial stress, regional differences can contribute to the risk of suicide in older adults. Individual factors, such as depression, anxiety, and other mental health disorders, as well as physical health problems and chronic pain, have also been associated with an increased risk of suicide in older adults. Understanding the social and individual factors that contribute to late-life suicide in both Latvia and Norway is essential for developing effective prevention and intervention strategies to address this issue. The novelty and aim of research on social and individual factors of suicide among older people lie in understanding the unique risk factors that contribute to late-life suicide both in urban and rural areas. While there is existing research on suicide in general, there are various factors that contribute to suicide risk in older adults. Regional differences are often different from those that affect younger populations. Therefore, research on social and individual factors of suicide among the older persons aims to identify these unique risk factors and develop tailored prevention and intervention strategies that address the needs of older adults. There is still a need for further research to understand the specific social and individual factors that contribute to this issue in rural and urban areas.

Developed transport and storage sector has up-most importance for production capacities in regions, especially in rural areas, ensuring supplies and deliveries, including well-developed and maintained infrastructure, transport vehicles, IT systems and human capital. Till 2040 demand for highly-educated and qualified logistics specialists will increase in Latvia due to growing labour costs, introduction of new technologies and digital solutions. The aim of the study is to identify the recent trends that give valuable findings for education and regional development policy elaboration and implementation in order to achieve balanced regional development promoting also rural development. The findings reveal that the higher the share of population with higher education the higher the wages. The regions with the larger share of population with higher education are mainly urban and pre-dominantly urban regions. However, transport and logistics sector is in all regions as well as demand for logistics specialists. Balanced regional development is crucial for sustainable development in national and the European Union level.

Innovations are very important condition and tool for competitive entrepreneurship. To obtain more effective approaches, researchers are devoting deepened analysis for many factors influencing innovations and innovative activities indicating that there is an important influence on different sources of information on innovations — own company, co-operation with universities and business incubators, with research organisations. Empirical research results were based on survey results of entrepreneurs and on analysis of obtained survey results by use of descriptive statistics indicators confirmed that entrepreneurs in Kurzeme region biggest inspiration get in their own company as well as from suppliers of equipment, programs and materials but they have less influence on information about innovations from universities and other higher education institutions as well as private organisations, also research organisations.

A regional analysis of performance indicators for Latvian business incubators provides a valuable insight into the performance of the business incubator ecosystem in various regions and helps to make future policy decisions on providing funding for the incubators that aim to support the growth and success of start-ups. The present research performed a regional analysis of operational programmes for two periods from 2007 to 2021. The strengths and weaknesses of Latvian business incubators across various regions of Latvia could be identified based on the analysis. This can help to make policy decisions and guide resource allocation to contribute to the development of the business incubator ecosystem in the future.

Ecosystem services (ES) have been widely researched for several years, but cultural ecosystem services (CES) have gained importance in recent years due to pressure on human well-being and public health. This literature review aims to continue research about ES assessment methods used in the valuation of landscape quality, analysing methods for CES assessment. The most assessed CES is aesthetic value being one of the most appreciated and widely known CES. Landscape quality assessment is complicated and rarely assessed, but several methods evaluate separate features of landscape quality, which gives an insight into accessible methods for landscape valuation. In this article, methods are analysed and categorised into four groups – economic, spatial evaluation, social and statistical analysis. Most analysed articles use several methods in one assessment giving more precise results. In CES assessment, the most used method is surveys and questionnaires and usually incorporating mapping methods to spatially explicitly represent the data.

Natural processes and human activity play a crucial role in altering the nitrogen cycle and increasing nitrogen oxide (N2O) emissions. Nitrous oxide isotopes 15N and 18O are important parameters that can help to explain the sources of N2O gas, as well as their circulation under different soil physical properties. The main goal of the study is to analyse the possibilities of using dinitrogen isotopes 15N and 18O, measured in soil samples, for the identification of N2O sources. A total of 16 plots were sampled. Each soil sample was assigned a code. Wetting of the samples was carried out to create wet aerobic conditions and wet anaerobic conditions. N2O measurements were performed in laboratory conditions using the Picarro G5131-i device. The 15Nα and 15Nβ values obtained in the measurement data were used to calculate the δ15NSP and δ15Nbulk values. The obtained δ15NSP and δ15Nbul values were analysed using two methods – descriptive statistics and Kruskal-Wallis test. The test showed that there are statistically significant differences between δ15NSP values (p-value <0.0001), and δ15Nbulk there was no significant difference (p-value 0.885).

Stable isotope ratios of water (δ2H and δ18O) are important indicators which describe hydrological processes in the environment. These parameters allow to analyse structure, status, migration of pollutants and processes of the groundwater system. Groundwater from dug wells is widely used as a drinking water source highlighting the importance of investigation of water quality and its possible flows and sources. Despite available centralized water systems, there are still wide territories where dug wells are the main source of drinking water supply. Dug wells are recharged from shallow groundwaters which makes them more vulnerable to anthropogenic contamination. Therefore, quality monitoring and estimation of anthropogenic influences are of importance. Quality requirements of drinking water do not provide measurements of stable isotope ratios in drinking water, but the combination of those elements can be valuable for the characterization of impacts on groundwater quality. The aim of this study was to survey 64 dug wells in Eastern Latvia and analyse water quality together with measurements of stable isotope ratios. Measurements were performed twice, i.e., autumn and spring to track water sources in wells and describe the extent of possible impacts. This approach can indicate the magnitude of local factor impacts on drinking water quality. Surveyed wells provide high differences in analysed parameters, for example, nitrate concentrations in autumn varies from 0.44 to 108.26 mg LE−1. Also, other detected parameters are variable; therefore, tracking water flow with stable isotope values gives better insight into the water quality of the studied region.