Fire blight disease caused by Erwinia amylovora is a destructive bacterial pathogen mainly on pears, apples and quinces from Rosaceae family. In this study, it was aimed determination of total protein amounts in different apple cultivars (Braeburn, Fuji, Gala and Golden), pear cultivars (Santa Maria and Williams) and quince cultivars (Eşme and Ekmek) in the infections of two virulent E. amylovora strains (Ea234-1 and Ea240-3) according as the time. It was taken leaf samples after leaf inoculation with E. amylovora (108 CFU ml-1) at 24th, 36th and 72nd hours. For verification of the infections, re-isolations were made from bacteria inoculated plants and the agent was identified as E. amylovora by biochemical, physiological and molecular tests. In determining the amounts of total protein and in the SDS-PAGE analyses were used Bradford and Laemmli methods, respectively, and absorbance values of protein extracts derived from the leaf samples taken, were obtained at 595 nm wavelength. According to the findings obtained; after infection of E. amylovora in the apple varieties comparing to controls, total protein concentrations at 24th hours increased and a decrease in the amount of 36th to 72nd hours and Braeburn has the highest protein content was determined. In the pear varieties, while total protein concentrations at 24th and 36th hours increased, a decrease in the amount of 72nd hour, and Santa Maria variety has the highest protein content was detected. In the quince varieties, total protein concentrations at 72th hour increased and Eşme variety has the highest protein content was identified. As a result of SDS-PAGE analysis, protein fractions which have different molecular weights were obtained. The protein bands were defined approximately 55-70 kDa and 35-55 kDa molecule weight on apple and quince varieties, respectively and also approx. 55-70 kDa in pear varieties.

In this study, the quince market was examined in the world and Turkey. The primary data in the study were obtained by questionnaire method with 28 middleman in different marketing levels in Sakarya, Antalya, Bursa, Bilecik and Denizli where quince production is concentrated. Secondary data were obtained from the FAO and TURKSTAT. The world quince production, which is 324465 tons compared to 1981-1985 which is the beginning of the period, increased by 94.3% to 630325 tons in 2013. Major countries in world quince production are Turkey, China, Uzbekistan, Morocco and Iran. Quince production in Turkey increased by 1.60 times compared to the average of 1981-1985. In this increase in the quince production in Turkey, the increase in the per-tree yield has been considerably effective (an increase of 40%). 5.3% of quince fruit produced in the world is exported. World quince exports amount increased 8.32 times and the value increased 38.42 times. The highest share in quince export values is in the Netherlands (33.6%). The Netherlands is followed by Turkey, Austria and Iran respectively. The share of Turkey in the world export value is 31.1%. Russia ranks first in the world quince import with 23.3% share. Austria and Germany are followed by Russia.9.4% of the quince production in Turkey is exported. The major quince producers are Sakarya, Bursa, Antalya, Bilecik and Denizli. The 15 of the interviewed intermediaries, reported that the most important problem that there is no quince at the desired time, quantity, quality and price (54).