It is necessary for developing strategies of integrated fertilization for crops to maximize production of crops and reducing the risk of pollution from chemical fertilizers. Therefore, the objectives of this study to evaluate the impact of bio-organic, chemical nitrogen on seed quality of canola. A study was conducted for two consecutive years (2005 to 2006) at the Farm of Faculty of Agriculture, Kafr El-Sheikh University, Egypt. Bio fertilizer (control and bio-fertilizer), compost (control, 6, 12 and 18 ton ha-1) and chemical nitrogen (control, 36, 72 and 108 kg N ha-1). The result indicated that, bio-fertilizer was significantly effects on protein % and iodine value except oil percent was decreased. On the other hand, the Maximum values oil and protein were recorded by using compost fertilizer at (18 ton ha-1). In respect to chemical fertilizer, adding nitrogen affected significantly on protein % and the maximum value was obtained at (108 N ha-1) while, oil percent and iodine value were decreased. Interestingly, it is clear that from results that cultivar belongs to canola (LEAR that contains < 2% erucic acid) and a nitrogen source has almost slight effect on oil physical properties. Totally, the interaction among treatments application compost (6ton ha-1) and (72 N kg ha-1) with combined bio- fertilizer was achieved suitable oil percent and could be improve canola seed yield and quality and environment would have a better condition.