Pleurotus tuber-regium has been used as fodder and traditionally used as medicinal supplement. The aim of this study was to screen in vitro proximate biochemical composition and nutritional potentiality of P. tuber-regium extract for further in vivo analysis of impact of the extract on serum lipid and testosterone profile of rats. Total cholesterol, HDL cholesterol, LDL cholesterol were measured photometrically. Testosterone was measured by chemiluminescence immune assay. P. tuber-regium extract contains different biochemicals such as proteins, carbohydrates, tannins, flavonoids, phenols etc. and showed highly quantified calorific value (297.89±1.92 calori/100g). Acute toxicity test showed no mortality and toxic behavioural symptoms. Significant decrease in total cholesterol (75.52±0.39mg/dL), LDL cholesterol (34.58±1.69mg/dL) and triglyceride (81.31±1.25) were observed in rat group treated with 200mg/kg dose of extract. 400mg/kg dose of extract showed higher efficacy than 200mg/kg dose of extract and significantly decreased total cholesterol (72.25±1.11mg/dL), LDL cholesterol (26.37±1.21mg/dL) and triglyceride (69.42±0.72). The extract showed testosterone enhance efficacy. 400mg/kg dose of extract showed high level testosterone (178.96±0.68 ng/dL) enhance efficacy than 200mg/kg dose (174.64±0.64ng/dL). Due to hypocholesterolaemia and testosterone elevating properties of P. tuber-regium extract, it can be said that, mushroom P. tuber-regium can be consumed as healthy diet supplement to maintain cholesterol level and to maintain good male reproductive health.

In Afghanistan most times people are drinking olive leave tea for prevention and decline of higher lipid and glucose level, therefore the objective of the current study was to find out whether olive leaf extraction is having any effect on blood metabolites. The Japanese quail has been used for biological studies because of its easy management and large numbers in limited area. In this experiment quails (100 male and 100 Female) were reared on a farm owned by the Department of Animal science of Agriculture faculty of Nangrahar University. Quails were kept under optimum environmental during rearing period (20- 22 C and 65 % relative humidity). The Adaptation period was for 10 days. Feed was given add libitum during 24 h. In this study we had five treatments T0 served as control without any water supplementation, T1 (1g/L), T2 (2g/L), T3 (3g/L) and T4 (4g of olive leaf / litter of drinking water). The Experiment continued for 4 weeks at the end of week. By increasing level of the olive leave powder in water blood glucose, cholesterol and triglycerides levels declined significantly from 509 to 422.5, 204-144.5 and 245 to 192.5 respectively

This study was conducted with the objectives to determine the effects of licorice root powder (Glycyrrhriza glabra) on performance, serum parameters, egg yolk cholesterol and antioxidant capacity in laying Japanese quail. Two hundred and forty 10-wk-old Japanese quails were randomly assigned to four dietary groups, each one four times replicated with fifteen quails per dietary groups. Control group fed the basal diet; other groups were fed basal diet supplemented with 0, 0.5, 1.0 and 1.5% licorice root powder. There were no significant differences in terms of final live weight, feed intake, feed conversion ratio, egg weight, serum cholesterol, triglyceride and High-Density Lipoprotein (HDL) concentration between treatment groups. Egg production was higher in control group and 0.5% licorice root powder supplemented groups. 1.0% licorice root powder supplemented group had lower egg production rate. Licorice root powder supplementation decreased Low Density Lipoprotein (LDL) concentration and increased glucose concentration significantly. Licorice root powder supplementation increased total antioxidant status (TAS) and reduced total oxidant status (TOS) and oxidative stress index (OSI). It was concluded from this study that supplementation of licorice root powder at the level of 0.5, 1.0 and 1.5% to the feed of laying quails had no adverse effects on performance. Licorice root powder supplementation can be used to reduce cholesterol level and increase antioxidant status in quails.

The aim of this study was to investigate the effects of different levels of alfalfa meal on performance, egg quality, egg yolk cholesterol and hatchability parameters of quail breeders. In this trial a total of 90 Japanese quail breeders (30 males, 60 females), 10 weeks old was distributed 5 experimental groups randomly. Each experimental group consisted of 6 replicates of 3 quails (1 males, 2 females) in each. During the 12 weeks experiment period, birds were fed with 5 dietary groups based on corn and soybean meal and containing 0 (control), 10, 20, 40 and 80 g/kg alfalfa meal. Feed and water were offered ad-libitum throughout the experiment. The different dietary levels of alfalfa meal had no significant effect on body weight change, egg production, egg weight, egg mass, feed conversion ratio, egg shell breaking strength, egg yolk triglyceride, fertility, hatchability of fertile eggs or hatchability of eggs set. The feed intake was decreased with 10 or 20 g/kg alfalfa meal levels in the diet. The eggshell weight was best in quails fed 20 g/kg alfalfa meal, and the egg yolk cholesterol was the lowest in quails fed 40 g/kg alfalfa meal. According to the results of this study the addition of 40 g/kg alfalfa meal in laying quail diets decreased the egg yolk cholesterol levels without adverse effect on performance.