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Cellular Bioenergetics in Spirulina platensis towards Growth and Phycocyanin Production Under Different Photon Flux Densities Using the Modified Zarrouk’s Medium 全文
2021
Anyway Chofamba
From all the pigments found in Spirulina platensis, phycocyanin has been found to have a diverse application in various fields, and has a high market demand, calling for a need to increase production and easy isolation methods. In general, phycocyanin production in cells depends on the light conditions, among other factors during the cultivation period. The focus of this study was to look at the effect of different light intensities on phycocyanin production in Spirulina platensis. Other cellular biochemical parameters, including chlorophyll content and protein, were explored under the different treatments. An experimental design containing 4 different light intensities of 20, 150, 300 and 600 μmol photons m2/s was administered with 3 replicates. The results obtained from the study showed that high phycocyanin content was obtained from a low light intensity treatment. Chlorophyll results were a bit in contrary to the results obtained for phycocyanin, with high chlorophyll content obtained in high light intensity treatments. Protein and biomass accumulation also followed the same trend, where they were observed to be higher in high light intensities, with the maximum biomass achieved at 600 μmol photons m2/s and maximum protein content achieved at 300 μmol photons m2/s. Due to the commercial potential of phycocyanin to humans, its low cost downstream cultivation and processing of Spirulina platensis will be of economic advantage to the relevant stakeholders to fulfil the rampant demands and affordability of the blue phycocyanin pigment to both first and third World countries, hence the need of producing phycocyanin using the modified Zarouk’s media which has cheaper if not affordable ingredients.
显示更多 [+] 显示较少 [-]Isolation and Molecular Identification of Some Blue-Green Algae (Cyanobacteria) from Freshwater Sites in Tokat Province of Turkey 全文
2017
Tunay Karan | Ramazan Erenler | Zekeriya Altuner
Collected blue-green algae (cyanobacteria) from freshwater sites throughout Tokat province and its outlying areas were isolated in laboratory environment and their morphological systematics were determined and also their species identifications were studied by molecular methods. Seven different species of blue-green algae collected from seven different sites were isolated by purifying in cultures in laboratory environment. DNA extractions were made from isolated cells and extracted DNAs were amplified by using PCR. Cyanobacteria specific primers were used to amplify 16S rRNA and phycocyanine gene regions using PCR. Phylogenetic identification of species were conducted by evaluation of obtained sequence analysis data by using computer software. According to species identification by sequence analysis, it was seen that molecular data supports morphological systematics.
显示更多 [+] 显示较少 [-]Isolation and Molecular Identification of Some Blue-Green Algae (Cyanobacteria) from Freshwater Sites in Tokat Province of Turkey 全文
2017
Tunay Karan | Ramazan Erenler | Zekeriya Altuner
Isolation and Molecular Identification of Some Blue-Green Algae (Cyanobacteria) from Freshwater Sites in Tokat Province of Turkey 全文
2017
Tunay Karan | Ramazan Erenler | Zekeriya Altuner
Collected blue-green algae (cyanobacteria) from freshwater sites throughout Tokat province and its outlying areas were isolated in laboratory environment and their morphological systematics were determined and also their species identifications were studied by molecular methods. Seven different species of blue-green algae collected from seven different sites were isolated by purifying in cultures in laboratory environment. DNA extractions were made from isolated cells and extracted DNAs were amplified by using PCR. Cyanobacteria specific primers were used to amplify 16S rRNA and phycocyanine gene regions using PCR. Phylogenetic identification of species were conducted by evaluation of obtained sequence analysis data by using computer software. According to species identification by sequence analysis, it was seen that molecular data supports morphological systematics.
显示更多 [+] 显示较少 [-]Respuesta fisiológica de cilantro (Coriandrum sativum L.) a la disponibilidad de agua en el suelo 全文
2014
Mejía de Tafur, María Sara(Universidad Nacional de Colombia sede Palmira Facultad de Ciencias Agropecuarias) | Marín Pimentel, Gilberto Eduardo(Universidad Nacional de Colombia sede Palmira Facultad de Ciencias Agropecuarias) | Menjivar Flores, Juan Carlos(Universidad Nacional de Colombia sede Palmira Facultad de Ciencias Agropecuarias)
En casa de malla de la Universidad Nacional de Colombia sede Palmira (3° 30' 45.6" N y 76° 18' 29.911" O, 1050 m.s.n.m., 23.5 °C y 77% de HR) se midieron los requerimientos hídricos del cilantro (Coriandrum sativum L.) variedad Unapal Precoso y se relacionaron con el rendimiento de follaje fresco y materia seca. Las plantas fueron sembradas en macetas con 6 kg de suelo y por ciclo de cultivo se aplicaron láminas de agua de 140, 160, 200 (testigo propuesta por Vallejo y Estrada, 2004), 240 y 280 mm. Se utilizó un diseño completamente al azar con cinco tratamientos, cuatro repeticiones y 36 plantas por unidad experimental. Cada día, durante la fase experimental, se realizaron mediciones de evapotranspiración y cada 35 días después de la siembra se midieron las producciones de follaje y biomasa. La mayor producción de follaje se obtuvo con 200 mm de agua, valor asociado con la mayor eficiencia en el uso de ésta, medida como producción de MS, (0.64 g/lt de agua aplicado). En la etapa de germinación el coeficiente Kc del cultivo (relación evaporación real/evaporación de referencia) fue de 0.83, 1.12 en la etapa de crecimiento lineal y 1.40 en la etapa de formación de tallo floral y cosecha. | An experiment was conducted to determine water requirements of coriander Unapal Precoso related to fresh foliage yield and dry matter. Plants were sown in pots with 6 kg of soil and water depths of 140, 160, 200 (witness proposed by Vallejo y Estrada, 2004), 240 and 280 mm were applied. A random complete block design was used with 5 treatments and 4 replications, with 36 plants per experimental unit. Daily evapotranspiration measurements were made. Production of foliage and biomass was evaluated 35 days after sowing. Increased production of foliage was obtained with 200 mm of water depth, value associated with greater efficiency in water use (0.64 grams of dry matter per liter of water applied). The crop Kc was determined: 0.83 at the stage of germination, 1.12 in linear growth stage and 1.40 on the stage of forming flower stems and harvesting.
显示更多 [+] 显示较少 [-]Physiological response of coriander (Coriandrum sativum L.) to water soil availability | Respuesta fisiológica de cilantro (Coriandrum sativum L.) a la disponibilidad de agua en el suelo 全文
2014
Mejia de Tafur, Maria Sara | Menjivar Flores, Juan Carlos | Marin Pimentel, Gilberto Eduardo
An experiment was conducted to determine water requirements of coriander Unapal Precoso related to fresh foliage yield and dry matter. Plants were sown in pots with 6 kg of soil and water depths of 140, 160, 200 (witness proposed by Vallejo y Estrada, 2004), 240 and 280 mm were applied. A random complete block design was used with 5 treatments and 4 replications, with 36 plants per experimental unit. Daily evapotranspiration measurements were made. Production of foliage and biomass was evaluated 35 days after sowing. Increased production of foliage was obtained with 200 mm of water depth, value associated with greater efficiency in water use (0.64 grams of dry matter per liter of water applied). The crop Kc was determined: 0.83 at the stage of germination, 1.12 in linear growth stage and 1.40 on the stage of forming flower stems and harvesting. | En casa de malla de la Universidad Nacional de Colombia sede Palmira (3° 30’ 45.6’’ N y 76° 18’ 29.911’’ O, 1050 m.s.n.m., 23.5 °C y 77% de HR) se midieron los requerimientos hídricos del cilantro (Coriandrum sativum L.) variedad Unapal Precoso y se relacionaron con el rendimiento de follaje fresco y materia seca. Las plantas fueron sembradas en macetas con 6 kg de suelo y por ciclo de cultivo se aplicaron láminas de agua de 140, 160, 200 (testigo propuesta por Vallejo y Estrada, 2004), 240 y 280 mm. Se utilizó un diseño completamente al azar con cinco tratamientos, cuatro repeticiones y 36 plantas por unidad experimental. Cada día, durante la fase experimental, se realizaron mediciones de evapotranspiración y cada 35 días después de la siembra se midieron las producciones de follaje y biomasa. La mayor producción de follaje se obtuvo con 200 mm de agua, valor asociado con la mayor eficiencia en el uso de ésta, medida como producción de MS, (0.64 g/lt de agua aplicado). En la etapa de germinación el coeficiente Kc del cultivo (relación evaporación real/evaporación de referencia) fue de 0.83, 1.12 en la etapa de crecimiento lineal y 1.40 en la etapa de formación de tallo floral y cosecha.
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