Fusarium, Rhizoctonia, Sclerotinia, Verticillium, Armillaria ve Rosellinia gibi toprak kökenli bitki patojeni funguslar, birçok bahçe ve tarla ürününde bitki ölümlerine ve verim kayıplarına sebep olan kök çürüklüğü hastalıklarına neden olmaktadır. Kök çürüklüğü hastalıklarının kontrolünde dayanıklı çeşit kullanımı, ürün rotasyonu, solarizasyon, fungisit uygulamaları ve toprak fumigasyonu gibi çeşitli mücadele yöntemleri uygulanmaktadır. Metil bromide ve kloropikrin gibi fumigantlarla yapılan toprak fumigasyonu toprak kökenli fungusların kontrolü için kullanılan en etkili yöntemlerden biridir. Ancak bu kimyasalların çevre ve insan sağlığına olumsuz etkilerinden dolayı dünyada ve ülkemizde kullanımı yasaklanmıştır. Bu nedenle, yeterli hastalık kontrolü sağlayan ve ticari fungisitlerle rekabet edebilen alternatif yöntemlerin araştırılmasına ihtiyaç vardır. Biofumigasyon kimyasal mücadeleye alternatif yöntemler arasında değerlendirilmekte nematodlar, yabancı otlar ve bazı fungusların neden olduğu hastalıkların mücadelesinde oldukça etkili olup, entegre mücadelenin bir parçası olarak kabul edilmektedir. Toprak kökenli fungusların kontrolünde örtücü bitkiler, organik gübre ve biyokatı atıklar biyofumigant olarak değerlendirilmektedir. Bu çalışmada toprak kökenli fungusların kontrolünde biyofumigasyon uygulamaları ve biyofumigantların funguslar üzerindeki etki şekilleri değerlendirilmiştir.

In this study, molecular markers were used to determine fungal flora in black olive fruits from field surveys to the table, following the fermentation process. Field samples were collected from different locations of Canakkale province, including Gokceada (Imbros), where organic farming is employed. Some of the fruits from field samples were used for black table olive production and then fungal flora was tracked during the fermentation process. Fungal isolation was also conducted on some commercial samples. Fifty seven isolates from field samples, 56 isolates from the fermentation process and 17 isolates from commercial products were obtained. Among these isolates, 41 Alternaria, 43 Penicillium, 19 Aspergillus, 8 Monascus and 19 other genera were determined using amplified sizes of the Beta-tubulin gene region. Species level identification was carried out based on sequences of Beta-tubulin amplicons, which provided accurate identification, especially where the genera were morphologically highly similar. The occurrence and prevalence of fungal species changed in fungal collections from the field to the fermentation process. While Alternaria alternata was common in field samples, they were absent during fermentation. Many of these identified species, such as Penicillium expansum, Aspergillus niger and Monascus pilosus, which are known as potential toxin producers such as aflatoxin, ochratoxin A and citrinin, were found both in natural and fermented samples, even at the end of the fermentation process. These results showed that some fungal species which survive on olives from the field to the table are potential toxin producers and can be successfully characterized by amplification and sequencing of Beta-tubulin gene.

Tomato is one of the most important vegetable crop which is widely grown in the world. Economic yield loses due to several insect species has been reported on tomato production. In recent years, a new pest Lasioptera sp, which causes significant damage in tomato production area has emerged. The pest has first been reported in tomato production areas of Mersin district in 2012 after it has been reported in Greece. This pest belongs to the family Cecidomyiidae and is causing damage in the stems and on the fruits of tomato. The larva feeds inside of the stem, so this makes management of the pest difficult. There are no adequate studies on integrated pest management on this pest. This report clearly showed that detailed studies on the biology and management of this pest in the tomato production should be done.