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The Determination of Their Efficiency Against Fusarium Root and Crown Rot Diseases of Some Antagonistic Trichoderma and Endophytic Acremonium Isolates
2018
Berna Tunalı | Cansu Tosun | Büşra Müge Maldar | Gonca Meyva | Bayram Kansu
The use of antagonist microorganisms against pathogenic organisms in organic agricultural systems was promising for biological control approach. The aim of this study was to determination of the potential biological control of some fungal isolates which characterized as endophytic and antagonistic against soil borne Fusarium culmorum (FC) and F. oxysporum f.sp. radicis lycopersici (FORL) pathogens. For this, seven isolates of endophytic Acremonium spp. that isolated from healthy wheat plants and eight isolates of antagonist Trichoderma spp., isolated from soil of organic vegetable areas, were performed as biological control agents (BCA). The agar media with hypha and mycelia of the BCAs and spore suspensions (106 spores/ml) of FC and FORL were treated for inoculation of wheat seeds and tomato seedlings. The harvested plants were statistically analyzed by some parameters (severity of crown rot disease (%DS and scale), plant lengths (cm) etc.). In conclusion, the Acremonium isolates (47.5%) were lower means of disease severity than Trichoderma isolates (74.6%) for FC and FORL assessments in wheat experiment. In tomato, the averages of disease severity between Acremonium and Trichoderma were similar while their means were lower than positive control (The Scale of DS=0.67
显示更多 [+] 显示较少 [-]Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum
2018
Işıl Melis Zümrüt | Berna Tunalı | Bayram Kansu | Gülşen Uz | Emre Yörük | Fatih Ölmez | Ayşegül Sarıkaya
Fusarium culmorum is predominating causal agent of head blight (HB) and root rot (RR) in cereals worldwide. Since F. culmorum has a great level of genetic diversity and the parasexual stage is assumed for this phytopathogen, characterization of isolates from different regions is significant step in food safety and controlling the HB. In this study, it was aimed to characterize totally 37 F. culmorum isolates from Turkey via sequence related amplified polymorphism (SRAP) marker based genotyping. MAT-1/MAT-2 type assay was also used in order to reveal intraspecific variation in F. culmorum. MAT-1 and MAT-2 specific primer pairs for mating assays resulted in 210 and 260 bp bands, respectively. 11 of isolates were belonged to MAT-1 type whereas 19 samples were of MAT-2. Remaining 7 samples yielded both amplicons. Totally 9 SRAP primer sets yielded amplicons from all isolates. Genetic similarity values were ranged from 39 to 94.7%. Total band number was 127 and PCR product sizes were in the range of 0.1-2.5 kb. Amplicon numbers for individuals were ranged from 1 to 16. According to data obtained from current study, SRAP based genotyping is powerful tool for supporting the data obtained from investigations including phenotypic and agro-ecological characteristics. Findings showed that SRAP-based markers could be useful in F. culmorum characterization.
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