This study was conducted to determine the associations of some milking traits and milk yield in water buffalo farms located in Bafra district of Samsun province. In total, 37 Anatolian Buffalo farms enrolled to Buffalo Breeders Association (BBA) were evaluated by applying face to face survey on the current milking practices and farm characteristics in February 2020. Number of milking cows (NM), number of workers (NW), age of the milker (AM), experience of the milker (EM), education of the farm owner (EO) and hygiene score of the milking parlor (HM) were evaluated as the farm traits affecting daily milk yield (dMY). Milking type (MT), milking frequency (MF), udder cleaning pre-milking (UC1), udder cleaning post-milking (UC2), giving feed after milking (GF) and milking duration (MD) were selected as the milking practices affecting dMY. It was concluded that dMY was significantly affected by AM, EM, EO and MD.