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The Effect of Different Applications on In vitro Bulb Development of an Endemic Hyacinth Plant (Hyacinthus orientalis L. subsp. chionophyllus Wendelbo) Grown in Turkey
2020
Selay Doğan | Gülat Çağlar | Esra Bulunuz Palaz
In this study the effects of different sucrose concentrations, and the combinations of jasmonic acid (JA) with auxins (IAA or NAA) or with cytokinin (2iP) on the bulb induction and rooting of in vitro plantlets of Hyacinthus orientalis subsp. chionophyllus Wendelbo, which is endemic in Turkey, were investigated. The effect of four different sucrose concentrations (30, 45, 60 and 90 g L-1) on bulb formation in tissue culture was investigated. These plantlets were cultured on MS medium supplemented with several concentrations and combinations of JA (0.0, 1.0, 2.0 mg L-1) and 2iP (0.0, 0.25 and 0.50 mg L-1), IAA or NAA (0.5, 1.0 mg L-1). In JA- 2iP treatment, the highest number of bulblets (13.7 number/explant) was obtained by the combinations of JA 1.0 mg L-1 + 2iP 0.25 mg L-1. Also, the largest bulblets with the mean diameter of 7.9 mm were found on MS medium supplemented with JA 2.0 mg L-1. In JA – Auxin treatment, the mean root number per bulblet was highest (17.9 number/explant) and root formation rate was maximum (81.14%) on MS medium supplemented with IAA 1.0 mg L-1 + JA 2.0 mg L-1.
显示更多 [+] 显示较少 [-]Effect of Leaf Segments and Potting Media on Plant Performance of Sansevieria trifasciata Hort. ex Prain Grown under Ex vitro Conditions
2019
Alaguthurai - Hematharshini | Thayamini Harold Seran
The present study was conducted to investigate the vegetative propagation of Sansevieria trifasciata under ex vitro conditions. The experiment was conducted at the net house to select the best leaf segment among apical, middle and bottom portions of leaves and also to evaluate their performance in different potting media. Leaf segments were taken from apical, middle and bottom portions and planted in soil: compost at 1:0, soil: compost at 1:1 and soil: compost at 1:2 ratios. After 90 days, number of shoots per cutting (>1cm), number of shoot buds (
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