During cheese ripening many biochemical reactions occur, including hydrolysis of fat, lactose metabolism, and protein breakdown. The aim of this study is to compare the direct measurement method from cheese with simple extraction methods in determining the hydrolysis of fats by FTIR spectroscopy. For this purpose, white cheese samples were produced, ripened for 60 days and analysed at 20-day intervals. The content of fatty acids was determined by gas chromatography, while its estimation was performed using Partial Least Squares Regression (PLSR). As a result, the PLSR performance obtained from fat extract samples by FTIR spectroscopy was shown higher values than the performance obtained from the direct measurement from cheese. It has been shown that simple extraction methods provide higher performance in FTIR spectroscopic measurements made from cheese.

In this study, sterol and fatty acid compositions with the other quality criteria (free fatty acids, peroxide value, total chlorophyll and carotenoid content) of olive oil samples obtained from Halhalı, Gemlik and Sarı Hasebi varieties through two phase mechanical method (crushing, kneading and centrifuge) was determined. Oleic, palmitic, linoleic, stearic, palmitoleic, linolenic and arachidic acids were the determined as the main fatty acids in olive oil samples. It was determined that oleic acid contents of oil samples ranged between 66.25-76.14% and Sarı Hasebi had the highest oleic acid content. Sterol and fatty acid compositions of olive oil samples showed significantly statistical differences according to varieties. It was determined that the total sterol contents of oils ranged between 1025 and 1686 mg/kg and varieties with the highest and lowest total sterol content were Gemlik and Sarı Hasebi. Apparent β-sitosterol contents (β-sitosterol, Δ-5-avenasterol, Δ-5-24-stigmastadienol, klerosterol, sitostanol) were between 92.96 and 94.63%. Varieties with the highest and lowest apparent β-sitosterol contents were oils which belong to Halhalı and Sarı Hasebi varieties respectively. β -sitosterol (83.08-88.21%), Δ-5-avenasterol (4.82-6.97%) and campesterol (2.28-3.43%) were identified as the main sterol components. Erythrodiol + uvaol contents of olive oils varied between 2.28 and 3.43% and these values were within the limits established by Turkish Food Codex.

Global olarak beslenme gibi yaşam tarzındaki hızlı değişim ile birlikte insülin direnci ve diyabet prevalansı hızla artış göstermiş ve günümüzde Tip II diabetes mellitus (Tip II DM) epidemik bir sorun haline gelmiştir. Yaşam tarzı müdahalelerinden en önemlisi olan diyetin düzenlenmesi ise tip II DM’nin ortaya çıkmasının engellenmesinde, geciktirilmesinde, komplikasyonların oluşmasında ve önlenmesinde önemli bir role sahiptir. Son yıllarda yapılan çalışmalarda Tip II DM gelişimine neden olan patolojik faktörler arasında yer alan insülin aktivitesi ve insülin sekresyonunun diyabet nedenini belirlemek açısından tek başına yetersiz kaldığı ele alınmaktadır. Buna ek olarak diyabet etiyolojisinde multi-faktöriyel nedenlerin, insülinin yanı sıra farklı reseptörlerin yer aldığı ve bu durumu genetik çalışmaların desteklediği bilinmektedir. Bu reseptörlerden biri olan CD36 reseptörü ise, kardiyovasküler sağlık ve hastalıklar, kanser, diyabet, insülin direnci, tat alma duyusu ve besin seçiminde önemli rol oynamaktadır. Yapılan çalışmalarda yüksek kan CD36 düzeylerinin tip II DM patogenezinde yeni bir belirteç olarak katkı sağlayabileceği yer almaktadır. Ayrıca güncel veriler CD36 reseptörünün yağlı besin seçimi açısından farklı tat ve kokularda görev alabildiğini göstermektedir. Bu derlemede CD36 reseptörü ile insülin direnci ve diyette yağlı besin seçimi arasında ilişki incelenmiştir.

During cheese ripening many biochemical reactions occur, including hydrolysis of fat, lactose metabolism, and protein breakdown. The aim of this study is to compare the direct measurement method from cheese with simple extraction methods in determining the hydrolysis of fats by FTIR spectroscopy. For this purpose, white cheese samples were produced, ripened for 60 days and analysed at 20-day intervals. The content of fatty acids was determined by gas chromatography, while its estimation was performed using Partial Least Squares Regression (PLSR). As a result, the PLSR performance obtained from fat extract samples by FTIR spectroscopy was shown higher values than the performance obtained from the direct measurement from cheese. It has been shown that simple extraction methods provide higher performance in FTIR spectroscopic measurements made from cheese.

In this study, sterol and fatty acid compositions with the other quality criteria (free fatty acids, peroxide value, total chlorophyll and carotenoid content) of olive oil samples obtained from Halhalı, Gemlik and Sarı Hasebi varieties through two phase mechanical method (crushing, kneading and centrifuge) was determined. Oleic, palmitic, linoleic, stearic, palmitoleic, linolenic and arachidic acids were the determined as the main fatty acids in olive oil samples. It was determined that oleic acid contents of oil samples ranged between 66.25-76.14% and Sarı Hasebi had the highest oleic acid content. Sterol and fatty acid compositions of olive oil samples showed significantly statistical differences according to varieties. It was determined that the total sterol contents of oils ranged between 1025 and 1686 mg/kg and varieties with the highest and lowest total sterol content were Gemlik and Sarı Hasebi. Apparent β-sitosterol contents (β-sitosterol, Δ-5-avenasterol, Δ-5-24-stigmastadienol, klerosterol, sitostanol) were between 92.96 and 94.63%. Varieties with the highest and lowest apparent β-sitosterol contents were oils which belong to Halhalı and Sarı Hasebi varieties respectively. β -sitosterol (83.08-88.21%), Δ-5-avenasterol (4.82-6.97%) and campesterol (2.28-3.43%) were identified as the main sterol components. Erythrodiol + uvaol contents of olive oils varied between 2.28 and 3.43% and these values were within the limits established by Turkish Food Codex.