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Prevalence and PCR Sensitivity Comparison of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in Salads and Appetizers Consumed in Istanbul 全文
2019
Zahide Bilgin | Gülay Merve Bayrakal | Emek Dümen | Gözde Ekici
This study was conducted to investigate incidence of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in 100 samples of salad and appetizers (50 salad and 50 appetizer samples) collected from retailers located various districts of Istanbul. Conventional microbiological methods and PCR procedures were used for Listeria monocytogenes and Staphylococcus aureus while only PCR procedures were used for the analysis of Toxoplasma gondii. Also PCR specifity and sensitivity for all the positive samples were calculated. According to the results, Listeria monocytogenes was detected in 9 samples (9%) and Staphylococcus aureus was detected in 36 (36%) samples while all the samples were negative for Toxoplasma gondii. PCR sensitivity results were quite specific and accurate for both Listeria monocytogenes and Staphylococcus aureus. It was concluded that salad and appetizers may be seriously threat consumers’ health microbiologically if they are processed under poor hygienic conditions.
显示更多 [+] 显示较少 [-]Comparison of the Sensitivity of Arabidopsis SOS Pathway Mutants under Salt Stress 全文
2019
Buasimuhan Abudureyimu | Emre Aksoy
Salinity stress is one of the most important and common abiotic stress factors that cause significant physiological and metabolic changes in plants, negatively affecting plant growth and development, and causing decrease in product quality and quantity. The elucidation of the molecular control mechanisms associated with salt stress tolerance is based on the activation and /or inactivation of various stress-related genes. Salt Overly Sensitive (SOS) tolerance mechanism under salt stress is of great importance in terms of salt tolerance of the plants. Although this mechanism has been studied for many years, the physiological changes that the plants give as a result of mutation of the genes in the pathway under different levels of sodium chloride (NaCl) during development have not been examined comparatively. In this study, we found that the Arabidopsis thaliana sos1-1 mutant plant showed sensitivity to 10 mM NaCl while the sos3-1 and hkt1-1 mutants showed tolerance. The sos1-1, sos3-1 and hkt1-1 mutants showed increasing sensitivity when NaCl was applied beyon 50 mM of concentration. In addition, plants did not show significant sensitivity for 1 day of stress application, while significant effects were observed in plant root length when exposed to salinity for 3 to 4 days. Col-0, hkt1-1 and sos3-1 roots treated with low levels of NaCl for a short term were positively affected in length. In the light of these results, the amount and duration of salt stress is very critical in Arabidopsis thaliana's responses to the stress and determination of molecular tolerance pathways.
显示更多 [+] 显示较少 [-]Kerevit (Astacus leptodactylus) Yemine Katılan Selenyumun Paraoksonaz ve Arilesteraz Enzim Aktivitelerine Etkisi 全文
2019
Serpil Mişe Yonar | Muzaffer Harlıoğlu
Bu çalışmada kerevit (Astacus leptodactylus Eschscholtz) yemine farklı oranlarda katılan selenyumun hepatopankreas ve gonad dokularında paraoksonaz (PON) ve arilesteraz (ARE) enzim aktivitelerine etkisi araştırılmıştır. Çalışmada, toplam selenyum düzeyi 0,3, 0,6, 0,9 ve 1,2 mg/kg yem olan sırasıyla; Kontrol (K), Deneme 1 (D1), Deneme 2 (D2) ve Deneme 3 (D3) yemleri oluşturulmuştur. Araştırma yemlerinin ham protein ve toplam enerji düzeyleri eşitlenmiştir. Çalışmada ebatları 2,0 × 2,0 × 1,0 m olan 12 adet havuz kullanılmıştır. Her bir havuza 75 dişi-25 erkek olacak şekilde toplam 1200 adet kerevit stoklanmıştır. Üç tekrarlı olarak yürütülen çalışmada kerevitler günde 2 öğün olmak üzere 9 ay süreyle yemlenmiştir. Kerevitlerden aylık olarak alınan doku örneklerinde PON ve ARE enzim aktiviteleri tespit edilmiştir. Deneme süresince hepatopankreas ve gonad dokularındaki PON ve ARE enzim aktivitelerinde istatistiksel olarak önemli farklılıklar bulunmuştur. Kerevitlerin dokularında üreme sezonu ve kuluçka süresi boyunca PON ve ARE enzim aktivitelerinin arttığı belirlenmiştir. Bu artışın kontrol grubuna kıyasla D1, D2 ve D3 gruplarında istatistiksel olarak farklı olduğu bulunmuştur. Sonuç olarak, selenyumun A. leptodactylus’un çiftleşme dönemi, yumurtlama dönemi ve kuluçka süresi boyunca PON ve ARE enzim aktivitelerini olumlu yönde etkilediği görülmüştür.
显示更多 [+] 显示较少 [-]Water-Yield Relationships in Deficit Irrigated Onion 全文
2019
Serhat Ayas
This trial was realized in the greenhouses of Uludağ University Yenişehir Vocational School between 2009 and 2010 to investigate effects of water deficit on yield and quality parameters of onion during four crop growth stages. In this trial, fourteen irrigation treatments in four growth periods (establishment, vegetative, yield formation and ripening) of onion (Allium cepa L E.T Grano.502) were constituted and the yield and quality parameters found from these treatments were evaluated. The layout of the experiment was a completely randomized block design with three replications for each of the fourteen irrigation treatments tested. According to the content of the treatments, the irrigation amount water applied to the plants varied between 0 and 436 mm in the first year, and between 0 and 448 mm in the second year. Water consumption of onion in the first year ranged between 205 and 496 mm and in the second year ranged between 210 and 502 mm. Yield, bulb weight, diameter, height and dry matter ratio were determined statistically significant. In 2009 and 2010 years, the maximum yield were found as 52.2 t ha-1 and 52.4 t ha-1 in E100V100Y100R100 treatments, while the minimum yield were found as 0.8 t ha-1 and 0.5 t ha-1 in the E0V0Y0R0 treatments, respectively. Water- yield relationship factors (ky) in 2009 and 2010 years were found as 1.03 and 1.04, respectively. The maximum WUE and IWUE values were obtained from establishment and ripening periods. Establishment and ripening periods may be suggested as the maximum efficient irrigation periods for the onion applied with drip irrigation under unheated greenhouse conditions.
显示更多 [+] 显示较少 [-]Establishing the Flow Charts that can be Used in the Design of Irrigation Automation Systems 全文
2019
Selçuk Usta | Serpil Gençoğlan | Cafer Gençoğlan
Otomasyon, insan müdahalesine ihtiyaç duyulmadan çeşitli cihaz, sistem ve programların işletilmesini sağlayan mekanik, elektronik ve bilgisayar tabanlı bir teknolojidir. Sulama otomasyonu sistemi; çevre, su kaynakları ve üretim maliyeti dikkate alınarak daha az insan gücü, daha kontrollü ve daha kaliteli tarımsal üretim ile planlanmalıdır. Sulama programını sera, tarla ve bahçe bitkileri yetiştiriciliğinde uygulama becerisine sahip olmalıdır. Sulama otomasyonu sistemi bazı işlem basamakları takip edilerek oluşturulmaktadır. Bunlar; sulama programının hazırlanması, sistem bileşenlerinin seçilmesi, projenin hazırlanması, yazılımın hazırlanarak test edilmesi ve sistemin kurulmasıdır. Bu aşamalardan geçirilen sulama otomasyon sistemi, sulama suyunun etkin kullanımını sağlar, işçiliği azaltır, sulama programını izler ve üretim maliyetlerini azaltır. Bu çalışmada, bitki su tüketimine ve bitki kök bölgesindeki nem düzeyine duyarlı sulama otomasyonu sistemlerinin tasarımına yardımcı olabilecek iş akış şemaları oluşturulmuştur.
显示更多 [+] 显示较少 [-]Assessment of Hygienic Quality of Beverage Cans Surfaces with and without Protective Cover 全文
2019
Gülten Tiryaki Gündüz | Ayça Korkmaz | İlkim Emenli | Zeynep Öztürk
In this study, the microbial profiles of external top surfaces of beverage cans sold in Izmir markets, and the survival of E. coli on the top surfaces of cans were investigated through the storage period of 0, 10, 20 and 30 days at 4 and 25°C. Total aerobic mesophilic bacteria (TAMB), coliforms and E.coli counts of 100 cans without protective cover and 20 cans with protective cover were examined. The numbers of TAMB on the cans without protective cover were in the range of 0.43-2.20×103 cfu/cm2. Lower bacteria counts in the range of
显示更多 [+] 显示较少 [-]Effects of Brining and Picking Time on The Degradation of Pesticide Residue in Grapevine Leaves 全文
2019
Rüstem Cangi | Yusuf Yanar | Yağmur Dülgeroğlu Yılmaz
Intensive pesticide use in vineyard resulted in residue problem on vine leaves that are used as food. This study was aimed at development of a proper chemical control program to reduce the pesticide residue problem on vine (cv. Narince) leaves in vineyards during the growing period. The residues of some fungicides were determined according to spraying time in the fresh (unprocessed) and preserved (brined) leaves. Additionally, the effects of preservation process on degradation of the fungucides residues were investigated. In this study three fungicides (Azoxystrobin, Triadimenol, Hexaconazole) were applied alternately for both powdery mildew and “Colomerus vitis” management, and two fungucides (Copper oxychloride, Metalaxyl + Mancozeb) for downy mildew control. Additionaly vine leaves were harvested at two different times: (i) before the half-life of the pesticides were reached and (ii) after the half-life of the pesticides have elapsed. Two different methods were applied to preserve the vine leaves. In first treatment, leaf samples were boiled in hot (98±2°C) tap water, then leaves were placed into jars, then filled with brine containing 8.0% salt + 0.25% lactic acid. In second treatment, vine leaves were placed into jars, then filled with tap water and brine containing 8.0% salt+0.25% lactic acid. The residue levels of the fungicides were determined on leaves. Detectable copper and the other fungucide residues are compared according to Turkish Food Codex. Preserving applications were decreased fungicide and copper residue levels and hot water brining was decreased the levels of fungicide residues between 75.2% and 99.2%, according to the applications. As a result, systemic fungicides should not be used in vineyards in where pickled vine leaves are produced. It is proposed that better to use contact fungicides instead of systemic one and also viticulturists should be careful using the effective contact fungicides.
显示更多 [+] 显示较少 [-]Assessment of Quality of Shea Butter Sourced from Organic and Inorganic Fields 全文
2019
Musah B Bawah | A K Afoko | A K Quainoo | A F Chimsah | Abudu Ballu Duwiejuah | A H Abubakari
The objective of the study was to compare selected physical and chemical composition of shea butter processed from nut sourced from inorganic and organic fields. The treatments were O (shea butter processed from nut picked from organic fields) and N (shea butter processed from nuts picked from inorganic fields). These treatments were arranged in a Complete Randomized Design and were replicated four times in the laboratory for studies of free fatty acids (FFA), pH, peroxide value, insoluble impurities, unsaponifiable matter, and moisture content. The study revealed that organic shea butter had lower free fatty acids (FFA), lower moisture content, lower pH, less unsaponifiable matter, less insoluble impurities and lower peroxide value as compared to inorganic shea butter shea butter. The lower FFA value of organic shea butter indicates that it can be used for the cosmetic and pharmaceutical industry and for direct consumption. Hence higher chances that organic shea butter and attract premium prices in both local and international markets. The study also revealed that there was a positive relationship between; unsaponifiable matter and moisture content. Insoluble impurities had a very strong correlation with unsaponifiable matter and moisture content. A very strong positive correlation was observed between peroxide value and unsaponifiable matter, moisture content, and insoluble impurities. For good quality butter, nuts should be sourced from organic fields.
显示更多 [+] 显示较少 [-]A Macroanatomic Study on Broncho-Pulmonary Segmentation in Hasak Sheep 全文
2019
Zekeriya Özüdoğru | Hatice Özdemir
In this study, 10 Hasak sheep lungs from Konya Bahri Dağdaş International Agricultural Research Institute, Unit of Experimental Animals Unit were used. Corrosion casting technique were applied to the materials. The corrosion cured materials were stored in water for one night. It was then incubated at 37 ° C in a 20% KOH solution for 24 hours. Corrosion casting technique and latex injection materials were photographed and presented in the study. The lungs were found to be wrapped with pleura visceralis (pleura pulmonalis) in the cavum thoracis. It was determined that the right lung was consisted of lobe cranialis (pars cranialis and pars caudalis), lobus medius, lobus caudalis and lobus accessorius. The left lung was composed of lobe cranialis (pars cranialis and pars caudalis) and lobe caudal. In the bronchus that shaped these lobes, it was determined that tacheae gave bronchus lobaris cranialis dexter (bronchus trachealis) mean 48.53 mm before the bifurcatio trachea, and later on it was divided into bronchus pirincipalis dexter and sinister. In this study, anatomical features of broncho-pulmonary segmentation and similarities and differences with other species were revealed.
显示更多 [+] 显示较少 [-]Effect of Drying Methods on Fatty Acid Profile of Hazelnut (Corylus avellana L.): A Review 全文
2019
Ali Turan
Hazelnut is the important agricultural product of the Eastern and Western Black Sea region of Turkey and is usually exported to the world market dried and unshelled. Hazelnut in husks are dried generally grass and concrete ground under the sun. The traditional sun-dried process (concrete and grass ground) used by hazelnut farmers takes about 10 to 25 days counting on the weather conditions. If rainfall is high during the harvesting period, drying takes longer and causes the harvest and spoilt. Rapid postharvest processing, mostly in husking and drying, is crucial for the quality the last product, among which the hazelnut drying plays essential role. Because sun-dried on the concrete and grass ground increase fungal activities of hazelnuts and the risk of mycotoxin development due to mould growth. And also, under the action light and heat lipid molecules are released free fatty acids, which can affect the stability of oil. Dryer can prevent mould growth and oil oxidation. Thus, drying machine appears to be a promising strategy for hazelnut drying.
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