Bu çalışmada, in vitro çoğaltma yöntemiyle nanelerin seri üretimi amaçlanmaktadır. İki farklı nane (Mentha x piperita L., Mentha pulegium L.) türünden eksplant örnekleri alınıp gerekli sterilizasyon işlemi yapıldıktan sonra 100 µL/L NAA (Naphthylacetic Asit) ve 600µL/L IBA ( Indol Butyric Asit) seviyelerindeki hormonlarla MS (Murashige & Skoog) ortamına alınmıştır ve 60 günde iki hafta arayla alt kültür yapılarak aktarma işlemi gerçekleştirilmiştir. Eksplantlardan aynı MS ortamında kök, gövde ve yaprak oluştuktan sonra naneler bitki büyütme odasında toprak ortamına alıştırılmıştır. Nanelerin kallus indüksiyonu 100 µL/L NAA ve 250µL/L BAP (Benzylaminopurine) hormon seviyeleri kullanılarak MS ortamına alınmıştır. Kallus kültürü için ortama alınan eksplantlardan sadece birinde (Mentha pulegium) kallus hücrelerinin indüklendiği görülmüştür. Mikroçoğaltım yöntemiyle geliştirilen her iki Mentha ssp’de (Mentha pulegium, Mentha x piperita) türünde de doğal yolla çoğaltma yöntemine göre ayda 6 kat daha fazla büyüdüğü görülmüştür. Elde edilen verilere göre mikroçoğaltım yöntemiyle geliştirilen Mentha x piperita ve Mentha pulegium türlerinin üretim potansiyelinin oldukça yüksek olduğu tespit edilmiştir.

The present study aimed to determine the heavy metal cadmium (Cd), chromium (Cr), nickel (Ni), lead (Pb) and mercury (Hg) accumulations in muscle tissues, gills, gonads, liver, and other visceral organs (liver) of whiting fish (Merlanguis merlangus euxinus Nordmann, 1840) samples obtained by hunting from the Sinop (Black Sea) coasts. The mean cadmium value in muscle tissue, which concerns public health, was found to be 1.07±0.02 µg.kg-1, the chromium value was found to be 1.48±0.07 µg.kg-1, the nickel value was found to be 1.77±0.14 µg.kg-1, the lead value was found to be 5.38±0.29 µg.kg-1 while the mercury value was determined only in the gill and could not be determined in organs, and since it was below the limit values declared by international organizations for all metals, it was determined that it would not create adverse effects for human consumption. Also, examining the order of accumulation of heavy metals in tissues and organs from highest to lowest, it was sorted as liver> visceral organ> gill> muscle> gonad for cadmium; visceral organ> gill> muscle> liver> gonad for chromium; visceral organ> gill> liver> muscle> gonad for nickel; gill> visceral organ> liver> muscle> gonad for lead, while the mercury accumulation was detected only in the gill. Also, the data obtained as a result of the analyses made in the gonad is an important determination in terms of the effects of heavy metals on the reproduction of the species, as well as on the continuity of the population. Since the values determined in the other analyzed tissues were below the limit values reported by international organizations, the results showed that they do not pose any threat to human health in terms of workplace and time, type, and determined heavy metals.

This study was aimed to evaluate DNA fragmentation by using Comet assay in naturally infected sheep with Anaplasmosis before and after treatment with the Comet method, which shows DNA damage specifically. In the study, blood samples were collected from 10 Anaplosmosis infected and 10 healthy sheep. The anaplosmosis was diagnosed by clinical signs and symptoms. The infection was confirmed by Giemsa staining. The blood was collected from control group and infected group before and after the treatment, from the vena jugularis with the appropriate method. The DNA fragmentation was checked by using the Comet assay of blood cells. The data were analysed throught ANNOVA one-way. The result showed higher DNA fragmentation in sick animals diagnosed with anaplasmosis; tail length and tail moment values were found to be statistically significantly higher than the control group. When the data obtained after imidocarb (IMD) application were compared with obtained during the disease, a decreased DNA damage and tail moment was determined, however, these values higher than control. In this study, DNA damage and the extent of this damage were investigated by the Comet assay method using a healthy control group before and after treatment in animals with Anaplasmosis. When the findings obtained from the study were evaluated, it was seen that Anaplasma agents caused DNA damage and with the imidocarb application given for treatment, DNA damage was reduced and results close to healthy individuals were obtained.

In this study, roots of Turkish origin wild chicory (Cichorium intybus L.) genotypes were investigated for total flavonoid and phenolic contents, radical cation scavenging activity (ABTS), Free radical scavenging activity (DPPH), and radical, mineral content. These characteristics were also compared with other coffee varieties. The total flavonoid and phenolic contents ranged between 0.290-4.350 mg QE/g dry weight (DW) and 0.943-13.860 mg GAE/g DW. The DPPH was listed here from high to low value: raw coffee beans = roasted coffee beans > roasted fruits of turpentine tree > instant coffee = roots of chicory. The content of P, Ca, Mg, Zn, B, Cr, Co and Mo ranged between 0.71-2.78%, 0.25-0.46%, 7.29-20.66, 4.44-11.07, 0.40-1.67, 0.49-5.48 and 5.69-14.46 ppm, respectively. As a result, chicory roots exhibited low antioxidant activity, but higher mineral content compared to the other tested coffee varieties which indicates that chicory could be used a coffee additive.

The need for studies on the nutritional value of plants depends on their composition. The first generation genetically modified Plants (GMPs) have the same composition as their near-isogenic lines. Therefore, they have the same nutritional value, and most of the animal feeding studies have found no significant differences in the production and health parameters of animals that consumed first-generation GMPs in comparison to non-GMPs. Due to the recent production of transgenic plants with specific nutritional properties (second generation GMPs), their use as feed for animals is viewed with skepticism in very many countries. In this critical review, it is concluded that most of these nutritionally improved plants have not shown adverse effects on the performance of various animals compared to their near-isogenic lines and can therefore be considered in the animal feed industry. However, most of the experiments were conducted on laboratory animals. There is a need to conduct them with animals that are mainly consumed by humans, such as ruminants. There is also a need to feed the whole plant to these animals and not just certain parts of the plant to get a clear picture of its overall safety. In addition, there is a need to determine a suitable long-term nutritional and toxicological approach assessment.

This study examined farmers’ level of use of climate change adaptation strategies across selected agro-ecological zones in Nigeria. Edo and Ondo States were selected for the study with three major agro-ecological zones. A multi-stage sampling procedure was used. The correlation analysis revealed a significant relationship between year of education and level of use of adaptation strategies (r = 0.15, P = 0.02). The result of Analysis of Variance (ANOVA) revealed that significant differences existed in most of the adaptation strategies employed except planting different varieties (F = 1.672, P = 0.190), intercropping (F = 0.646, P = 0.525), crop rotation (F = 2.436, P = 0.090) and migration to different sites (F = 0.661, P = 0.517). The study recommended that the issue of climate change should not be taken lightly by all stakeholders as its effect differs among various agro-ecological zones.

Global climate change is defined as a process that affects all living things and ecosystems globally and is claimed as the most critical problem of the current century. Turkey, which is shown as one of the most affected countries by this process, is among the “countries at risk.” It is stated that the temperature will increase throughout the country until 2100, and this increase may reach 6 ºC. In order to determine the possible effects of global climate change, it is necessary to predict how the climate structure and basic parameters may change. From this point of view, this study is aimed to determine the change of temperature and precipitation, climate types (according to De Martonne, Lang, and Emberger climate classification) which are the most critical climate parameters until 2050 and 2070 in Düzce, one of the important cities of our country. The current situation and possible changes in 2050 and 2070 have been compared using RCP 4.5 and RCP 8.5 scenarios. As a result of the study, the temperature, precipitation, and related climate types would change significantly throughout the province of Düzce, and this change will show itself as a significant temperature increase and change in precipitation regime. In addition, a shift in climate types towards continental climate types is predicted until 2070. In order to avoid the destructive effects of global climate change, it is recommended to take measures on a sectoral basis.

Son yıllarda gerek tarımsal ilaçların gerekse kimyasal gübrelerin bilinçsizce kullanımı bitkisel üretimde artışın yanında kalitesiz ve insan sağlığını tehdit edecek ürünlerin ortaya çıkmasına neden olmuştur. Söz konusu sorunlara karşı sürdürebilir tarım ve değişik tarım alternatifleri konusunda çalışmalara ihtiyaç olduğu görülmektedir. Bitkilerin farklı tarım uygulamaları altında en yaygın sorununun azot (N) kullanım şekli olduğu görülmektedir. Bitkinin N kullanım etkinliğinde, uygulanan gübrenin N dozu ve formunun önemli olduğu düşünülmektedir. Bu amaçla, sera koşullarında artan dozlarda (0 (kontrol), 100, 200, 400 ve 800 mg N kg-1) ve farklı formlarda (mineral, organik) N uygulamalarının buğday bitkisinin büyüme, yeşil aksamda klorofilin bir ifadesi olan SPAD değeri, yeşil aksam kuru madde verimi ve yeşil aksam N konsantrasyonu üzerine etkisi belirlenmiştir. Denemeden elde edilen bulgulara göre, N noksanlığından kaynaklı verim kayıplarının olduğu buna karşılık N uygulamasının söz konusu verim kayıplarının önüne geçtiği saptanmıştır. Azot uygulamasından kaynaklı verim artış oranları üzerine uygulanan N formunun da önemli olabildiği belirlenmiştir. Özellikle mineral gübre uygulamasında verim artış oranlarının %58 ile %87 arasında değiştiği buna karşılık organik kaynaklı gübre uygulamalarının verim değerlerinde kısmen düşüşe neden olduğu görülmüştür. Farklı formlardaki N uygulamaları, bitkinin yeşil aksam kuru madde veriminin aksine yeşil aksam N konsantrasyonunu arttırmıştır. Bu artışlarda, N uygulama dozunun önemli olduğu buna karşılık uygulanan gübre formunun önemli bir farklılık yaratmadığı belirlenmiştir. Sonuçlar genel olarak değerlendirildiğinde en etkin N uygulama dozunun 200 mg kg-1 olduğu, bunun dışında N formları içinde bitkinin büyümesinde ve N kullanım etkinliğinde en etkin formun organik kaynaklı gübre olduğu görülmüştür.

Microalgae, also called phytoplankton by biologists, are very small plant-like organisms with a diameter of 1-50 micrometers without roots, stem and leaves. Microalgae, which have hundreds of thousands of species in both fresh waters and seas, form the lowest link of the food chain in aquatic ecosystems. Most species contain chlorophyll, use sunlight as an energy source, and convert carbon dioxide into biomass (biomass). Because of their role in the photosynthesis process, microalgae produce most of the oxygen in the atmosphere. It has a very wide biodiversity and is reported to contain more than 200 thousand species. As a result of genetic analysis, a continuous increase in microalgae species is observed. More than 15 thousand new chemical compounds have been discovered from algae in recent years. It has been observed that most of the bio compounds obtained from microalgae have antiviral effects. However, although extensive research has been done on the antibacterial, antioxidant and antifungal effects of these bioactive compounds, there is limited research on their antiviral effects. In these limited number of studies on the antiviral effects of microagines, it has been reported that some biocompounds isolated from algae may be effective against viruses that are the cause of diseases such as “HIV, SARS and AIDS”. However, the number of researches on viruses that cause today's biggest pandemic, such as coronavirus, of different biocompounds isolated from microalgae, is very small. To date, no vaccine that can be effective against the COVID-19 virus or a drug that can inhibit the reproduction of the virus has not been found. It is thought that micro or macro algae may be one of the most promising natural resources in solving this global health problem. Because Spirulina, which is a microalgae, has antiviral, anticancer, antidiabetic, antibiotic, antioxidant, prebiotic, cardiovascular system protective and antiallergic effects and these positive effects are caused by bioactive compounds found in high content (Rosales-Mendoza et al., 2020a). In this review, especially the antiviral effects of microalgae were tried to be summarized and it was tried to be emphasized that algae could be promising natural resources in the development of new antiviral drugs by our country's scientists.

Bu araştırmada, Tarım ve Orman Bakanlığı, Yozgat Avcılık ve Yaban Hayatı Şube Müdürlüğü Üretim İstasyonunda 1240 metre rakımda yetiştirilen 42 haftalık yaşta olan Beç Tavuklarına (Numidae meleagris) ait 200 adet yumurta kullanılmıştır. Bireysel yumurta ağırlıkları ile birlikte, 5184×3456 piksel boyutunda 72 piksel/inç çözünürlükte görüntüleme yapılmıştır. Elde edilen görüntüler üzerinden ortalama Objektif Alan (16,07 cm2), Çevre (15,82 cm), Dairesellik (0,81), Yükseklik (5,17 cm), Genişlik (4,04 cm), Gri Değeri (82,82), Taban Yarıçapı (2,02 cm), Uzun yarı yüksekliği (2,96 cm), Kısa yarı yükseklik (2,20 cm) değerleri sayısal görüntü analizleri yapılarak belirlenmiştir. Elde edilen veriler üzerinden ortalama Elongasyon (1,28), Şekil İndeksi (78,27) hesaplanmıştır. Bireysel yumurta ağırlıkları kullanılarak ortalama yüzey alanı (55,43 cm2), boy (5,16 cm), en (3,77 cm), elongasyon (1,37), şekil indeksi (73,01), hacim (40,14 cm3), yüzey/hacim oranı (1,38), kabuk ağırlığı (3,17 g), kabuk kalınlığı (0,28 mm), gözenek sayıları (6666,25; 5132,39; 5011,12 adet), gözenek yoğunlukları (120,32; 92,56; 90,31), sarı oranı (14,85), sarı ağırlık (5,95 g), ak ağırlığı (30,75 g), ak oranı (77,21) gibi parametreler hesaplanmıştır. Gri değeri bakımından yumurtalar 90, Şekil indeksi bakımından yumurtalar 79 ve ağırlık bakımından 43 olacak şekilde 3 gruba ayrılmış, her grubun diğer özellikler üzerine etkisi araştırılmıştır. Sonuç olarak elde edilen verilerin bilimsel çalışmalarda kullanılabileceği düşünülmektedir.