Alchemilla genus, which belongs to the Rosaceae family, is a medicinal plant used for various purposes among the people. Species of this genus are known in Turkish folk medicine as lion claw or hazelnut grass. Especially, they are used mainly women’s illnesses, in gastritis, anti-inflammatory, as carminative, and in the treatment of wound. Besides the antimutagenic effect of Alchemilla alpina L., its above-ground parts are used for antimycotic purposes in the form of tea or oral care water. In this study, it has been aimed to determine the antimicrobial effect of the above-ground parts of Alchemilla alpina extracts obtained from methanol, ethanol and chloroform and the antioxidant activity of different concentrations of the extract obtained from methanol. The antimicrobial activity of methanol, ethanol and chloroform extracts of the above-ground parts of A. alpina has been determined according to disk disc diffusion method. In the results obtained have been showed that these extracts inhibited the growth of some bacteria (Staphylococcus aureus ATCC25923, Escherichia coli ATCC25322, Klebsiella pneumoniae ATCC700603, Bacillus megaterium DSM32) and yeasts (Candida albicans FMC17 and Candida glabrata ATCC66032) at different rates (8-23 mm). The antioxidant activity of different concentrations (1.25, 2.5, 5 and 10 mg/ml) of the above-ground parts of A. alpina extract obtained from methanol has been determined according to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity method. In the results obtained, it has been observed that the effect of removing DPPH radical of A. alpina increased depending on increasing concentrations.

Salmonella is known as one of the main factors of food-borne gastroenteritis, and with this feature, it poses a great risk in terms of public health and economic losses. It is estimated that of the approximately 94 million cases of salmonellosis occurring in the world each year (about 85 percent of these are food-borne), an average of 150,000 result in death. Current social trends highlight the important health benefits of fresh produce in our daily diet. As an irony; Uncooked consumption of fresh herbal products is increasingly identified as a source of transmission for pathogens of intestinal origin, and epidemics occurring in this way are spreading rapidly. Today, the frequency of anthropogenic pathogen outbreaks associated with fresh produce, spices, and nuts has surpassed those associated with foods of animal origin. Human pathogens in the production chain; It can be transferred to plant material by basic means such as the use of animal manure, contaminated irrigation water, biological vectors (insects and animals) and contaminated seeds. In the light of these data, many intestinal pathogens have been identified as phyllo sphere-associated bacteria. Despite all these findings, there are many unknowns on the persistence and infectivity of Salmonella in contaminated plant hosts. In this review article, the factors affecting the attachment, colonization and survival of Salmonella on plant surfaces, as well as the information on the infection processes that continue with the invasion of plant tissues are discussed in the light of contemporary literature data.

Since coliform bacteria are common both in the intestine and in nature (soil, plant, etc.), they are considered as a sanitation indicator in the food industry. It is known that the majority of bacteria defined as fecal coliform in the coliform group are Escherichia coli. The presence of E. coli or fecal coliform bacteria in any sample is an indication that the necessary hygienic measures are not taken during production, storage, and sale. That means the sample is directly or indirectly contaminated with faeces, and/or other intestinal pathogens may also exist. In this study, raw milk, freshly squeezed fruit juices, unpackaged ice cream, shaved ice, and ice-cold samples were purchased from the famous touristic destination Fethiye and analyzed for coliform bacteria. For this purpose, the samples were purchased from local marketplaces, buffets, cafes, patisseries, restaurants, and roadsides at Fethiye. In total 60 samples were analyzed using Violet Red Bile (VRB) Agar. The results of coliform bacteria ranged

The study investigated the potentials of date fruit as sweetening agent in bread and determined micronutrient composition, antioxidant properties and inhibition of diabetic related enzymes such as the alpha amylase and glucosidase. The fibre and ash contents of the samples increased with the addition of date fruit. The phosphorous, calcium, potassium, iron and magnesium contents of the bread sweetened with date fruit pulp were higher when compared to the bread samples that contained granulated sugar. Bread samples that contained date fruit exhibited greater DPPH radical scavenging activities, metal chelating activities, FRAP and possessed higher inhibition against α-amylase and glucosidase when compared with bead that contained granulated sugar. The results also showed greater antioxidant and enzyme inhibitory activities in the bread sweetened with dry date fruits that the wet date fruits. The sensory results showed that bread sweetened with granulated sugar and the date fruits were not significantly different from one another. The overall results showed date fruit has dual potentials in bread, as sweetener and as ingredient in enhancing nutritional qualities of bread.

Covid-19, which affects the whole world and is accepted as a pandemic by the world health organization, caused millions of people to die. The elderly are in the group with the highest risk of losing their lives due to the Covid-19 virus. Stricter restrictive / protective measures have been taken for the elderly in Turkey, as in the whole world. With this research, it is aimed to evaluate the sociological and psychological effects of the "elderly", who are one of the health risk groups of the society, in terms of landscape architecture, during the pandemic days when they had to experience various problems after the outbreak. In the study, face-to-face and online questionnaires were applied to individuals aged 50 and over on a voluntary basis. As a result of the study, it was determined that the implementation of curfews and restrictions for the elderly and the formation of an isolated lifestyle from other age groups caused an increase in loneliness and anxiety levels and negative psychological effects. It has been revealed that the activities that individuals will carry out in the green area, their conversations and socializing with their peers, and walks in the open area have a positive psychological and sociological effect for the elderly. It has been determined that the elderly who become lonely and depressed at home with the restrictions imposed under pandemic conditions want to be in green areas. The sociological and psychological positive effects of being in green areas and spending time, and the changes in the behaviour and emotions of the elderly who have pandemic restrictions paralleled the response of the participants to the questionnaire.

Bağışıklık sistemi vücudu hastalık yapıcı patojenlere karşı koruyan temel savunma sistemidir. Bu savunma sistemi farklı mekanizmalarla etkili olmaktadır. Bağışıklık sistemi, antijen adı verilen vücudun kalıtsal yapısına yabancı olan her türlü yapıya karşı etki gösterir. Vücuda dışarıdan gelen hastalık etkenleri öncelikle doğal bariyerlerle karşılaşmaktadır. Doğal bariyerleri aşmayı başaran etkenler kemik iliği, timus, lenf bezleri ve dalak gibi özelleşmiş organlarda üretilen savunma hücreleri ile karşılaşır. İlk aşamada makrofajlar ve fagositler devreye girer daha sonraki aşamada ise B ve T lenfositleri sürece dahil olurlar. B lenfositlerinden salgılanan antikorlar bağışık sisteminin en önemli savunma mekanizmalarından birini oluşturmaktadır. Antikor moleküllerinin vücudun savunmasındaki bu önemi bilim insanlarını bu alanda çalışmaya yöneltmiştir. Bu doğrultuda 1975 yılında Georges Köhler ve Cesar Milstein, koyun alyuvarları ile immunize ettikleri farelerin B lenfositleri ile fare myeloma hücrelerini birleştirerek oluşturdukları hibrit hücreler ile yeni bir tedavi stratejisinin kapısını açmışlardır. Bu hibrit hücreler sadece istenen antijene bağlanan monoklonal antikor sentezleyebilme yeteneğine sahiptir. Monoklonal antikorlar tıp dünyasında hastalıkların teşhisi, tedavisi ve biyokimyasal analizler gibi birçok alanda kullanmaktadır. Günümüzde hala monoklonal antikora dayalı tedavi ve tedavi seçenekleri üzerinde çalışmalar devam etmektedir. Bu derlemede monoklonal antikorlar ve tedavide kullanımı üzerine genel bilgiler verilecektir.

The roles of protein in bodybuilding and the regulation of biological processes are important in sustaining life. A large amount of protein is required by both humans and animals and this cannot be supplied by only conventional sources. This is because of the rapid increase in world population. The present sources of protein will not meet global protein demand in years to come. Scientists explore the production of single-cell protein (SCP), as an alternative source of protein, through the utilization of wastes and low-value materials. SCP can supply high-quality protein containing both essential and non-essential amino acids that can be utilized by humans and animals. Protein from microbial biomass is cheaper than animal proteins because the substrates used in the production are generally cheaper and more readily available. Moreover, the production process does not require arable land and the entire process can be completed within a short time. This article reviewed the process of SCP production. Different raw materials used in the production and variations in growth media preparation methods were discussed. Various sources of fermentation microorganisms and their potential substrate were reviewed. Growth media enrichment using different carbon, nitrogen, and mineral sources was also discussed.

This study aimed to determine the L-DOPA content in the flowers of some different faba bean genotypes and determine the L-DOPA levels in tea prepared from flowers. The experiment was carried out under the ecological conditions in Samsun by the Black Sea with 15 genotypes using a randomized complete block design in three replications. The flowers were harvested three times and the number of flowers and flower yields were determined. The L-DOPA content of the flower and flower tea were determined using HPLC. Different solvents were used to extract the L-DOPA from the faba bean flowers. As a result of the HPLC analyses, the highest L-DOPA yield was determined to be in the tea samples brewed with hot water. It was found statistical differences between genotypes in the second and third harvests for the number of flowers in the plant and the total number of flowers. Dry flower yields ranged from 11.33 to 37.78 kg da−1 while L-DOPA levels were 6.2 to 9.17 g 100g−1 in dry flowers and 6.69 to 9.23 g 100g−1 in infused tea. The study concluded that flower tea of faba bean can be investigate for medicinal purposes and that L-DOPA in the plant can be extracted by brewing without requiring any solvent. This shows that L-DOPA is in a salt form within the plant.

Nowadays, immediate environmental friendly solutions such as the use of biopesticides and other methods to control and manage pests are well needed. They are imperative due to the continuous accumulation of toxic residues from synthetic insecticides into the environment, the contamination of global agro-ecosystem and resistance of certain insects and pathogens. The global renewed interest of botanical pesticides does not leave aside Turkey. Thus, many environmental reports pointed out contaminations in different regions of Turkey by chemical pesticide residues, like lambda-cyhalothrin in some conventional grapes farming in the Aegean region. The use of botanical pesticides like Pyrethrin extracted from Pyrethrum’s flowers (Chrysanthemum cinerariifolium) is part of sustainable agriculture goals to reduce the incidence of pests and diseases without any alteration to the natural balance. The purpose of this review is to analyse and identify the possibilities of Pyrethrum production in Turkey. Various research works around the world were compiled and some key informants were correlated to existing researches in Turkey. Subsequently, it was found that the Black-Sea region of Turkey has great potential in the growing and industrial production of Pyrethrum by its weather patterns (temperatures, pluviometry and humidity) and soil characteristics. The region has a similar climate with the East-African high-lands well-known for Pyrethrum production. Thus, the country has shown great technological advances and the capacity to produce vegetative and disease-free clones for other ornamental plants by using tissue culture techniques. Therefore, the multiplication of high-quality vegetative clones of Chrysanthemum cinerariifolium and their maintenance can sustain commercial and long term production of Pyrethrum in Turkey.