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Effect of Pollination with Gamma Irradiated Pollen on In Vitro Regeneration of Ovule Culture in Cyclamen
2022
Mehmet Tütüncü | Yeşim Yalçın Mendi
The obtention of homozygous lines through in situ parthenogenesis via gamma irradiation method is a comprehensive application in vegetables. However, there are a limited number of studies on in situ parthenogenesis in ornamentals. Therefore, in situ parthenogenetic capacity of more species needs to be examined. For this purpose, the effects of pollination with gamma-irradiated pollen on in vitro ovule cultured in Cyclamen persicum L. were evaluated in this study. Flower buds were collected before anthesis and irradiated at different doses 50, 100, 150, 200, 300, and 450 Gy of gamma-ray using a Co-60 source. The control group was pollinated with non-irradiated pollen. Fruits were harvested 30 days after pollination, and isolated ovule explants were cultured on four different mediums in in vitro. M0 was control group containing half-strength MS basal media; M1 media additionally has 10 g/L maltose, 1.0 g/L proline, 2.0 g/L peptone, 200.0 mg/L spermidine and 0.5 mg/L kinetin; in addition to basal medium, M2 media additionally contains 10 g/L maltose, 1.0 g/L proline, 2.0 g/L peptone, 200.0 mg/L spermidine, 0.4 mg/L gibberellic acid (GA3) and 0.4 mg/L N6-benzyl adenine (BA). M3 media additionally contains 2.0 mg/L 2,4-D and 0.8 mg/L 6-( γ, γ-dimethylallylamino) purine (2iP). Plantlets started to form 10-12 weeks after the beginning of culture. The effects of nutrient media, irradiation dose, and their interactions on plant formation were statistically significant. The lowest plantlet regeneration (0.33%) was obtained from ovule explants exposed to 50 Gy gamma rays and cultured on M3 media while the highest plantlet regeneration (2.66%) was obtained from ovule explants cultured on M1 media 30 days after pollination with non-irradiated pollen grains. According to stomatal observations, there were no statistical differences between donor plant and in vitro regenerated plantlets.
显示更多 [+] 显示较少 [-]Present Status and Future Prospects of Tea production and Research on Varietal Improvement in Bangladesh
2022
Md. Riyadh Arefin | Md. Ismail Hossain
Tea is one of the most important non-alcoholic beverage in the world and has been gaining further popularity as an important ‘health drink’ in view of its inferable medicinal value. In Bangladesh, commercially tea was cultivated since 1854 by establishing first tea garden Malnicherra Tea Estate in Sylhet. From 1947 to 2020, tea growing area, production and per hectare yield were increased 127.71%, 370.53% and 137.96% respectively. The major reasons behind the increasing tea productivity are extension of tea growing areas as well as cultivation of the tea clones (BT clones) released by Bangladesh Tea Research Institute (BTRI). Till now BTRI released 23 clones and 5 biclones, having average per hectare yield of 3461.67 kg. In the world, tea production, consumption and exportation have increased significantly. Unlike world condition, there is a negative relation between tea production and export in Bangladesh. From 1980 to 2020 the quantity of tea exports was decreased by 92.99%. This situation emphasizes the need for exploring alternative means by the tea industries of increasing profits from tea cultivation and tea export. As a result, researches on tea varietal improvement is needed for rapid economic growth and development of tea industry as well as to encourage tea plantation business to go ahead with more production to meet our own demand along with quality tea to flourish tea business in Bangladesh.
显示更多 [+] 显示较少 [-]Phosphate Solubilizing Bacteria (PSB) Isolation from Tomato Rhizospheres at Koka District, Ethiopia
2022
Dereje Haile | Bizuayehu Tesfaye | Fassil Assefa
Phosphorous is an essential macronutrient for plant growth and development. Most of the soil is deficient in plant available phosphorous and due to economic limitations majority of Ethiopian farmers applied inadequate fertilizers. It is essential to stabilize a mechanism to access P for plants with an efficient, cheap, and eco-friendly approach for enhanced crop growth and production. The main objective of this study was to screen efficient phosphate solubilizing bacteria from tomato rhizosphere soil. Using halo zone formation on PVK agar medium, more than 400 PSB isolates were isolated from 13 rhizosphere soil samples. By evaluating SI, texture in the culture-re-culturing process, liquid medium pH change efficiency and growth rate, upmost three promising PSB isolates (K-1-29, K-10-27, and K-10-41) were selected. Incubation of the isolates in PVK broth for five days showed significant pH reduction. For instance, isolate K-10-41 showed significant pH change (4.02) which indicates the organic acid production. Isolation and evaluation of efficient phosphate solubilizing bacteria maintain soil fertility, promote plant growth, induce plant response to pathogens, reduce agrochemical consumption and promote sustainable agriculture. Therefore, these selected PSB isolates need further detailed study for taxonomic identification, plant growth promotion, host range, and phytopathogen response. Local isolation improves environmental adaptation and indigenous competition.
显示更多 [+] 显示较少 [-]Solid Waste Landfill Site Selection; Konya City Sample
2022
Büşra Altay | Zekeriya Can Erbil
In developing countries, consumption habits have changed with the increase in population, the development of technology and industrialization. For this reason, good management of the increase in the amount of solid waste by municipalities is very important for the health and sustainability of nature. The location of the facility to be established is important for the regular storage and efficient disposal of solid wastes. Site selection of landfill facilities in the ‘Site Management and Operation Guide for Landfill Facilities’ published by the General Directorate of Environmental Management; The ratio of the volume to the area, the distance to the buildings, the wind direction, the external view (landscape), the effect on the traffic on the side, the profit from the finished facility, and the effect on the water are evaluated under the sub-headings. With the ‘Konya Solid Waste Management Project’, ‘Aslım Sanitary Landfill’, which was used until 2017, was closed. Instead, the ‘Konya Solid Waste Landfill Site’, which was established in a different region to meet the need, was put into operation. In the study to be carried out in this context; Both fields were evaluated according to the specified criteria and compared with each other using the Analytical Hierarchy Method. As a result, it is aimed that the success rate of the location selection of the new solid waste storage area will be revealed and it will be a base for similar studies to be done in the future.
显示更多 [+] 显示较少 [-]Molecular Investigation and Phylogenetic Analysis of Ehrlichia canis in Dogs in Siirt, Turkey
2022
Burçak Aslan Çelik | Özgür Yaşar Çelik | Ali Bilgin Yılmaz | Adnan Ayan | Özlem Orunç Kılınç | Ramazan Özdemir | Özge Oktay Ayan
Ehrlichia canis is the primary etiologic agent of canine monocytic ehrlichiosis, a tick-transmitted disease of dogs. The aim of this study is to molecularly investigate the presence of E. canis and to reveal its prevalence in dogs in Siirt province. The animal material of the study is consisted of a total of 82 dogs. A region of the 16S ribosomal RNA gene of E. canis was targeted for PCR amplification. As a result of the conducted Nested-PCR, positivity was detected at the rate of 10.53% (4/38) in male dogs and 13.64% (6/44) in females, and Ehrlichia canis specific bands of size 389 bp were obtained in 10 (12.20%) dogs in total. The phylogenetic tree was constructed with the Maximum Likelihood (MCL) method, The nucleotide sequence was registered in the NCBI GenBank database with access numbers OK331365.1-OK331366. Early detection of the disease by means of hematological, serological, or molecular tests is very important in terms of prognosis. More studies should be performed to determine vector-disease relationships in this region about ticks that vector the disease.
显示更多 [+] 显示较少 [-]Investigation of the Changes in Rheological Properties of Purple Basil Sherbet Samples Concentrated by Ohmic Heating at Different Voltage Gradient
2022
Zeynep Eroğlu | Mutlu Çevik
Purple basil is widely used medicinally and aromatically due to its essential oil properties, and it is a plant that stands out with its antibacterial, antimutagenic and antioxidant properties. It has been determined that purple basil has positive effects on health such as appetizing, insomnia, sputum, diuretical and antispasmodic. Purple basil contains high concentrations of anthocyanins and with these properties it stands out as a potential new source of stable red pigments for the food industry. In recent years, it has been seen that purple basil has been processed into different products such as tea and sherbet in order to benefit from its functional properties. With the processing of purple basil into different products, it is seen that the consumption of basil and basil products has increased by consumers. In this study, purple basil sherbet samples were concentrated at different voltage gradient values (14, 17 and 20 V/cm) under atmospheric conditions with ohmic heating up to 25% soluble solid content (SSC) and the changes in their rheological properties during the concentration process was investigated. The changes in the rheological properties of the purple basil samples were determined in the range of 1-148 s-1 shear rate values and measurement temperature was 25°C. The compatibility of different rheological models (Newton, Power-Law and Herschel-Bulkley) to the experimental data was statistically evaluated to determine the flow behavior index of the purple basil sherbet samples. It has been determined that the shear stress values increased as the shear rate values increased in all the process conditions. Similarly, at the same shear rate values, it was determined that the shear stress values increased as the SSC values increased. It has been determined that the best suitable rheological model was Herschel-Bulkley Model for all purple basil sherbet concentrates. It is thought that the results obtained will provide important data to the food and machinery industry for the installation of pilot and industrial scale ohmic heating systems.
显示更多 [+] 显示较少 [-]Effect of Pink Rock Rose Extract with or Without Ascorbic Acid and Sodium Ascorbate for the Preservation of Ready-to-Eat Frankfurter Type Sausages
2022
Ayça Gedikoğlu | Hale İnci Öztürk | Sencer Buzrul | Münevver Sökmen | Ezgi Aytaç
This study aimed to determine the effect of pink rock rose extract (PRR) with or without ascorbic acid and sodium ascorbate for improving the shelf life of sausages. Analyzed parameters were DPPH radical scavenging capacity of PRR extract; total aerobic count, thiobarbituric acid reactive substance, heme iron, pH, water activity, proximate composition, and color values of MAP packaged sausages for 12 weeks at 4 °C. Treatments: (1) Control (0.02% ascorbic acid and 0.05% sodium ascorbate – AA-SA), (2) electrostatic spray application of PRR extract (2%) – ES-PRR, (3) 0.02% AA and, 0.05% PRR extract, (4) 0.05% SA and 0.02% PRR, (5) 0.07% PRR extract. PRR extract had the half maximal inhibitory concentration (IC50) value of 13.04 ± 0.133 µg/mL. Sausages formulated with 0.07% PRR had the lowest microbial growth rate, followed by AA-PRR formulation. The AA-PRR treatment had the lowest TBARS values for most of the storage. This study reveals that PRR extract can be added as a natural antioxidant in sausages, and it could be used as a replacement or for the reduction of ascorbic acid and sodium ascorbate in sausage formulations.
显示更多 [+] 显示较少 [-]Evaluation from Rural to Urban Scale for the Effect of NDVI-NDBI Indices on Land Surface Temperature, in Samsun, Türkiye
2022
Burcu Degerli | Mehmet Çetin
In this study, in order to evaluate the change of LST from rural to urban scale in 20 years, a zoonal statistical analysis was performed by separating the urban and rural districts located on the coastline. Algorithms were applied to the raw data of Landsat 8 and Landsat 7 satellite images, using the Arc Gis 10.2 and Q Gis 3.16 utilities. In this way, NDVI, NDBI and LST data were compared and evaluated in terms of rural and urban districts. The correlation coefficient between the parameters was calculated. In the study, the land change between the years 2000-2020 was also determined to reveal the land change. As a result of the analyzes made, the amount of green areas decreased by 14.1% between 2000 and 2020 in the study area, which includes the central districts of Samsun, İlkadım and Atakum, and in the rural areas, Bafra and Ondokuz Mayıs. It has been observed that this rate is shared as 7.1% in built up areas and 7.33% in bare soil areas. Considering the effect of the decrease in green areas on the LST value, in 2000, max. While LST is 41.75 C, in 2020 max. It is seen that LST has increased to 43.44 C. When the districts were analyzed separately, it was seen that LST established a strong correlation with NDBI (positive) and NDVI (negative) for all four districts. However, the correlation was stronger in rural districts. It was observed that the correlation strength weakened in urban districts due to heterogeneous land surface cover.
显示更多 [+] 显示较少 [-]Developing a New Methodology for the Use of GIS and AHP in Determining Suitable Areas for Wheat Plants in the Lower Kelkit Basin
2022
Doğaç Sencer Yılmaz | Hakan Mete Doğan
In agricultural production planning; compiling the data correctly, and using and interpreting the data precisely have strategic importance. This study aims, it is aimed to develop a model that can evaluate the suitability of the Lower Kelkit Basin for wheat farming by using the Analytical Hierarchy Process (AHP) and current GIS technologies. In the study, weight values of seven different criteria of topography (slope, aspect) and soil (texture, organic matter (OM), CaCO3, EC, pH) were calculated with AHP. These weight values and standardized criteria maps were combined within the ArcGIS Weighted Overlay tool and the result maps were created according to the FAO suitability index. According to these maps, 54% of the Lower Kelkit Basin was modeled as unsuitable (N) for wheat, 22% as moderately suitable (S2), and 24% as highly suitable (S1). In addition to all these, an editable and updatable ArcGIS model tool was also produced as a result of the study. Our results indicated that AHP and GIS are powerful and effective tools that can be used in land suitability modeling.
显示更多 [+] 显示较少 [-]Truffle Checklist of Turkey II with A New Record
2022
Ilgaz Akata | İsmail Şen | Mustafa Sevindik | Şanlı Kabaktepe
The current study presents a Turkish Truffle Checklist based on literature and a newly discovered species. Within the two divisions, the list includes 104 species belonging to 35 genera and 20 families, as well as their range and Turkish names. Tuber oligospermum was one of them, and it was recorded for the first time in Turkey. A brief description of newly reported species was provided, along with images of their macro and micro-morphology.
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