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Ranjana Sinha(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Livestock Production and Management) | Mukesh Bhakat(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Tushar Kumar Mohanty(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Raj Kumar(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Ashish Ranjan(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Animal Genetics and Breeding) | Abdul Rahim(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India)) | Shabir Ahmad Lone(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Nadeem Shah(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Adil Rasool Paray(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Chandrashekhar Santosh Patil(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India)) | Ajeet Singh(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre)

The present study was undertaken to identify the difference in sperm kinematics in Murrah buffalo bulls in different seasons. Eight Murrah buffalo bulls maintained at Artificial Breeding Research Centre, ICAR-NDRI Karnal. Frozen semen samples of the bulls were obtained and sperm kinematic parameters were measured by computer-assisted sperm analyzer. The sperm kinematic parameters included curvilinear velocity (VCL), linear velocity (VSL), average path velocity (VAP), distance of average path (DAP), distance straight line (DSL), straightness coefficient (STR), linearity (LIN), amplitude of lateral head displacement (ALH), and beat cross frequency (BCF). Morphological abnormality of sperm includes bent tail (BT), coiled tail (CT), distal droplets (DD) and distal mid-piece reflex (DMR). Analysis revealed that the proportion of BT was significantly (P LT 0.05) lower in winter and autumn season as compared to summer and rainy season. Percent of total motile and progressive motile sperm was significantly (P LT 0.05) higher in winter season as compared to summer and rainy season. The VAP, VSL and VCL of sperm motion were significantly (P LT 0.05) higher in winter and autumn season as compared to the summer season. The DAP and STR were significantly (P LT 0.05) higher in winter and autumn season as compared to summer and rainy season. DSL and LIN were significantly (P LT 0.05) lower in summer and rainy season followed by autumn and winter season. Post-thaw motility and motion parameter varied significantly between seasons, and higher sperm motility was maintained in semen cryopreserved in winter followed by autumn, rainy and summer season.

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