The application of a bionematicide based on chlamydospores of Pochonia chlamydosporia (Pc-10) can be an important strategy for reducing the damage caused by Meloidogyne incognita on carrot. Based on this perspective, the nematicidal effects of 0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 kg ha-1 of Pc-10 were evaluated on carrot cv. Juliana under field conditions. Carrot yield and nematode population were influenced by increasing doses of Pc-10. The application of 3.0 kg ha-1 of Pc-10 increased the marketable production of carrot roots by 41.7% compared to the untreated control, whereas the production of unmarketable roots and the nematode population in the soil were reduced by 48.7% and 61.4%. The application of 3.0 kg ha-1 of Pc-10 reduces M. incognita population and improves carrot quality and yield.

Essential oils have been extensively used in many commercial applications, one of them being anesthetics. The effect of four essential oils (tea tree, clove, eucalyptus, and mint oils) on the sedation, recovery, and behavioral stress of Nile tilapia ( Oreochromis niloticus ) was evaluated. The sedative solutions, prepared with essential oils and anhydrous ethanol (1:4), were used for fish management procedures at a dose of 7.4 mL L - 1 in a completely randomized block design with four treatments and seven replicates. The means were compared using Scott – Knott test (P < 0.05). Clove oil was found to be the most suitable oil for the immobilization of Nile tilapia; however, behavioral observations indicate that tea tree oil was the most efficient in reducing stress.

Stream water quality is dependent on many factors, including the source and quantity of the streamflow and the types of geology and soil along the path of the stream. This study aims to evaluate the origin and the mechanisms controlling the input of ions that effect surface water quality in the sub-basin of the Rio das Cobras, Rio Grande do Norte state, Northeastern Brazil. Thirteen ponds were identified for study: three in the main river and ten in the tributaries between, thus covering the whole area and lithology of the sub-basin. The samples were collected at two different times (late dry and rainy periods) in the hydrological years 2009 and 2010, equating to total of four collection times. We analyzed the spatial and seasonal behavior of water quality in the sub-basin, using Piper diagrams, and analyzed the source of the ions using Guibbs diagram and molar ratios. With respect to ions, we found that water predominate in 82% sodium and 76% bicarbonate water (cations and anions, respectively). The main salinity control mechanism was related to the interaction of the colloidal particles (minerals and organic sediment) with the ions dissolved in water. Based on the analysis of nitrates and nitrites there was no evidence of contamination from anthropogenic sources.

This study aimed to assess the effect of nitrogen (N) and potassium (K) fertilisation on the forage accumulation and nutritive value of Urochloa ruziziensis during the summer of 2010/2011 and autumn, winter, spring and summer of 2011/2012. A complete randomised block design with four treatments (0, 120, 240 and 360 kg ha-1 year-1 N and K2O) and five replicates were used. The data were analysed under the PROC MIXED of SAS®. The following variables were evaluated: sward height; forage mass; forage bulk density; percentages of leaf blade dry matter, stem dry matter, and dead material dry matter; leaf blade:stem ratio; contents of dry matter and crude protein; in vitro digestibility of dry matter; neutral detergent fibre content; and lignin content of Urochloa ruziziensis. There was an interaction between the N and K levels of fertilisation and the season for all variables. In addition, there was a positive linear effect of the N and K levels of fertilisation on the forage accumulation rate, the crude protein content and the in vitro digestibility of dry matter and a negative linear effect these levels on the neutral detergent fibre and lignin contents during the spring and summer of 2011/2012. N and K fertilisation and the climate changes characteristic of each season jointly affected the accumulation and nutritive value of Urochloa ruziziensis. N and K fertilisation up to 360 kg ha-1 year-1 improved the forage accumulation and nutritive value of Urochloa ruziziensis.

Aggregate formation and stability are related to soil quality, contributing significantly to the carbon storage and nutrient maintenance capacities of the soil. Soil aggregates are formed by two different process: physicogenic, related to moistening and drying cycles and input of organic matter; and biogenic, related to the action of macrofauna organisms and roots. The objective this work was to classify aggregates according to their formation process, quantify and compare organic carbon contents in humic substances and assess the stability of aggregates formed by different processes, in areas with different coverage in the Mid Paraiba Valley, Pinheiral, State of Rio de Janeiro, Brazil. Aggregated soil samples were collected at a depth of 0-10 cm, in a Cambisol (Cambissolo Háplico Tb Distrófico) under four plant covers: secondary forest in advanced (SFAS), medium (SFMS) and initial (SFIS) successional stages and managed mixed pasture (MMP). Aggregates were classified and identified into three morphological classes (physicogenic, biogenic and intermediate). The variables evaluated were mean weight diameter (MWD) and geometric mean diameter (GMD) of aggregates, chemical fractions of organic matter, total organic carbon (TOC) and humic substances: humin (C-HUM) humic acid (C-FAH) and fulvic acid (C-FAF). Biogenic aggregates were found in smaller quantities and showed higher TOC, C-HUM and C-FAH, compared to intermediate and physicogenic aggregates. Thus, biogenic aggregates have potential to be used as soil quality indicators for structured environments, which are able to maintain its intrinsic formation processes.

The objective of this work was to morphologically characterize 15 banana cultivars and assess the physical and chemical characteristics of their fruits at two maturation stages, unripe (pre-climacteric) and ripening. The plants were evaluated regarding their pseudostem height and diameter, petiole length, leaf blade length, width and length-to-width ratio. The cultivar Ouro had fruits with lower diameter, total length, market weight and fresh weight at both stages, and also firmer pulp when they were unripe. The cultivar Caru-Roxa had higher fresh fruit and pulp weights, and the cultivar Terrinha had the highest percentage of pulp dry weight percentage in unripe and ripe fruits. The cultivars Maçã and Ouro had higher pulp-to-peel ratio in unripe fruits. The ripe peels had lower fresh weight and thickness and higher dry weight percentage compared to unripe peels. The fruit peel of the cultivar Marmelo had the highest fresh weight at both stages. The cultivars Marmelo and Maçã had higher percentage of peel dry weight percentage at both stages. The unripe pulp had lower soluble solids. The titratable acidity in the pulp increased with ripening. The average plant height ranged from 2.25 to 6.15 m. The cultivars that had the largest pseudostem diameters had also the highest heights, except the Prata-Anã and Prata-Graúda. The cultivar and maturity stage influenced all the characteristics evaluated in fruits, except the total and market lengths, which did not vary with the ripening of fruits.

This study aimed to evaluate the performance, carcass yield and intestinal morphometry of 1- to 21-day-old broiler chickens fed a diet containing increasing levels of sunflower cake, supplemented with or without an enzyme complex (EC). We used 1200 Cobb 500 birds, which were distributed in a completely randomized design in a 2x5 factorial scheme and five levels of sunflower cake inclusion (0, 5, 10, 15, and 20%) in diets supplemented or not with EC. Each treatment consisted of 5 replicates. Animals were fed the experimental diets from 1 to 21 days of age and from 22 to 42 days all birds received the same feed based on corn and soybean meal. There was no interaction between the level of sunflower cake inclusion and presence of EC. From 1 to 21 days of age, the weight gain, final weight, and feed intake linearly decreased (P<0.05) with increasing sunflower cake inclusion level. At 42 days old, after animals consumed diets based on corn and soybean meal (from 22 to 42 days), the levels provided least 21 days did not influence the performance results. Animals receiving the EC in the first phase showed better results at the end of the experiment (P<0.05). Up to 21 days old, carcass yield decreased, and abdominal fat increased with the sunflower cake inclusion. The addition of EC in the diet improved the thigh, breast and carcass yield. Intestinal morphology after 21 days revealed that the three segments of the intestine had at least one type of change, such as a decrease in villus height and increase in crypt depth, with increased level of sunflower cake in the diet, affecting performance.

The rate of swine wastewater application (SW) in agricultural production could result in the replacement of chemical fertilizers. However, SW destroys soil physical properties by decreasing pore bulk, which negatively affects both crop yield and development. In this context, this study aimed at monitoring the influence of swine wastewater as a cover fertilizer in maize and black oats in sequence on soil physical properties. Five application rates (0, 100, 200, 300 and 537 m3ha - 1 equivalent to 0, 11.2, 22.3, 33.5 and 60 kg ha-1 N, respectively, based on the average nitrogen concentration in SW) were tested with four replications each. In the studied area, soil porosity, density, and water content, before maize sowing and at the end of the cycles of maize and black oats, were determined by the volumetric ring method. Data were submitted for regression analyses. There was a reduction in the macroporosity and total porosity of the soil when the SW application rate, before maize cultivation, was higher. The introduction of black oats helped to improve the physical quality of the soil and reduced the compaction of the surface layer from 0 to 15 cm and 100 to 300 m3 ha-1 SW rates.

Passiflora cincinnata Mast. has become more popular in the market because the unusual flavor of its fruits and natural beauty of its flowers, and has great potential for breeding programs of Passiflora edulis f. flavicarpa, because its resistance to diseases and drought. The objective of this work was to evaluate seven wild passion fruit (P. cincinnata) accessions, using morphological and agronomic descriptors and molecular markers type ISSR, to identify their morphoagronomic and genetic variabilities and potential for use in breeding programs. A randomized block experimental design was used with five replications and two plants per plot. Thirteen qualitative and twenty-one quantitative, vegetative and floral characteristics were used for morphoagronomic characterization. Twelve ISSR primers were evaluated for molecular characterization. Among the qualitative characteristics, only the color variations were significantly different between the accessions. According to the mean squares of the quantitative characteristics evaluated, obtained from analysis of variance, the means of accessions showed significant differences (p<0.01) for all characteristics. The IAL (internode average length) was the morphological descriptor that most contributed to diversity, with 43.12%, followed by DH5 (stem diameter at 5 cm height) and SW (sepal width). The average genetic similarity found was 68%. Despite the low genetic variability found among accessions, the primers UBC-887 and UBC-841 stood out with high percentage of polymorphism with 14 and 11 polymorphic fragments, respectively, and higher values of polymorphism information content (PIC), resolving power (RP) and marker index (MI), denoting suitability for use in diversity studies of P. cincinnata. Low variability was found among accessions evaluated.

The objective of this work was to evaluate the effects of the application of phosphogypsum and vinasse on soil chemical properties and productivity and nutritional characteristics of alfalfa (Medicago sativa). The experiment was conducted in a randomized block design, using a 3×5 factorial arrangement, with three vinasse rates (0, 150 and 300 m3 ha-1) and five phosphogypsum rates (0, 3, 6, 9 and 12 Mg ha-1). The alfalfa chemical composition and shoot dry matter (SDW) and soil chemical properties (in the layers 0.0-0.2 and 0.21-0.4 m) were evaluated. The vinasse rates increased the soil potassium contents, while the phosphogypsum rates promoted linear increases in soil calcium and sulfur contents. The base saturation was increased and the magnesium content showed a quadratic response on the layer 0.21-0.4 m with the increase in phosphogypsum rates. The calcium, magnesium and phosphorus contents in the alfalfa leaves were lower with vinasse application. The phosphogypsum rates promoted linear increases in alfalfa SDW. Vinasse rated 150 m3 ha-1 was been enough to SDW increase. Calcium and magnesium contents in the leaves fitted a quadratic model, with maximum calcium content in the phosphogypsum rate of 9.5 Mg ha-1 and the minimum magnesium content in the phosphogypsum rate of 8.7 Mg ha-1. The leaf sulfur contents in all vinasse rates and leaf potassium contents in the highest vinasse rate showed maximum accumulation at near 9 Mg ha-1 of phosphogypsum.