This study aimed to evaluate the in vitro behavior of the fungus Fusarium guttiform in different conditions of temperature and luminosity and determine the most efficient inoculation method for evaluating the resistance of pineapple to fusariosis. To determine the in vitro behavior, two experiments was performed, with the same design, conduction, and evaluation. It was used a completely randomized experimental design in a factorial arrangement of 3x3 (three temperatures and three light regimes), with five replications. The evaluations of inoculation methods were conducted in a completely randomized design with factorial arrangement 3x4 (methods x distance of inoculation). D leaves of pineapple, cultivar Pearl, were inoculated at two, five, eight and eleven centimeters from the base by the methods of infected toothpick, mycelium disc without injury and mycelium disc with injury on the leaf. There was no significant difference on in vitro behavior of the isolates analyzed. The indicated temperature and luminosity for the multiplication of F. guttiform were 25°C and 12 hours, respectively, since they showed significant my celial growth and increased production of reproductive structures. The indicated method for evaluation of resistance to fusariosis in pineapple was the inoculation by infected toothpick at a distance between 2 - 11 cm from the base of the leaf. It becomes necessary to perform an injury at the site to be inoculated for the infection of the pathogen F. guttiform occurs.

RESUMO: O presente estudo objetivou avaliar o comportamento in vitro do fungo Fusarium guttiforme em condições de temperatura e luminosidade distintas e determinar o método de inoculação mais eficiente para a avaliação da resistência de abacaxizeiro à fusariose. Para a determinação do comportamento in vitro foram realizados dois experimentos utilizando para cada o delineamento inteiramente casualizado, em esquema fatorial 3x3 (três temperaturas e três regimes de luminosidade), com cinco repetições para avaliação do índice de crescimento micelial e produção de conídios. As avaliações dos métodos de inoculação foram realizadas em delineamento inteiramente casualizado, com arranjo fatorial 3x4 (métodos x distância de inoculação) a partir de folhas "D" de abacaxizeiro da cv. Pérola, inoculadas a 2, 5, 8 e 11 centímetros da base pelos métodos palito infestado com estruturas do patógeno, disco de micélio sem ferimento e disco de micélio com ferimento na folha. Não houve diferença significativa no comportamento in vitro dos isolados analisados. A temperatura e fotoperíodo recomendadas para a multiplicação de F. guttiforme foram de 25°C e 12 horas, respectivamente, por apresentar maior índice de crescimento micelial e produção conídios. O método indicado para avaliação de resistência à fusariose do abacaxizeiro por meio de inoculação foi o palito infestado com estruturas do patógeno, a uma distância de 2 a 11 cm da base da folha. Para que ocorra infecção do patógeno F. guttiforme faz-se necessário a realização de ferimento no local a ser inoculado. | ABSTRACT: This study aimed to evaluate the in vitro behavior of the fungus Fusarium guttiform in different conditions of temperature and luminosity and determine the most efficient inoculation method for evaluating the resistance of pineapple to fusariosis. To determine the in vitro behavior, two experiments was performed, with the same design, conduction, and evaluation. It was used a completely randomized experimental design in a factorial arrangement of 3x3 (three temperatures and three light regimes), with five replications. The evaluations of inoculation methods were conducted in a completely randomized design with factorial arrangement 3x4 (methods x distance of inoculation). D leaves of pineapple, cultivar Pearl, were inoculated at two, five, eight and eleven centimeters from the base by the methods of infected toothpick, mycelium disc without injury and mycelium disc with injury on the leaf. There was no significant difference on in vitro behavior of the isolates analyzed. The indicated temperature and luminosity for the multiplication of F. guttiform were 25°C and 12 hours, respectively, since they showed significant my celial growth and increased production of reproductive structures. The indicated method for evaluation of resistance to fusariosis in pineapple was the inoculation by infected toothpick at a distance between 2 - 11 cm from the base of the leaf. It becomes necessary to perform an injury at the site to be inoculated for the infection of the pathogen F. guttiform occurs.

– The downy mildew, caused by the fungus Pseudoperonospora cubensis is a major foliar disease that attacks the melon crop in the rainy season in northeastern Brazil. The objective of this study was to identify and characterize morphologically resistant melon accessions to P. cubensis. Thirty-six accessions and four commercial cultivars were evaluated in a randomized block design with three replications. The plot was composed of seven plants. The evaluation was conducted in the under field conditions with a diagrammatic scale and calculating the disease percentage index (DPI). Resistance sources and references genotypes were characterized morphologically. The accessions C-RN-2, C-SE-2, C-CE-2, C-PE-2, C-PI-1, and C-BA-2 show resistance to P. cubensis and are promising for use in programs breeding for resistance to downy mildew although its fruits have reduced commercial quality.

ABSTRACT: The downy mildew, caused by the fungus Pseudoperonospora cubensis is a major foliar disease that attacks the melon crop in the rainy season in northeastern Brazil. The objective of this study was to identify and characterize morphologically resistant melon accessions to P. cubensis. Thirty-six accessions and four commercial cultivars were evaluated in a randomized block design with three replications. The plot was composed of seven plants. The evaluation was conducted in the under field conditions with a diagrammatic scale and calculating the disease percentage index (DPI). Resistance sources and references genotypes were characterized morphologically. The accessions C-RN-2, C-SE-2, C-CE-2, C-PE-2, C-PI-1, and C-BA-2 show resistance to P. cubensis and are promising for use in programs breeding for resistance to downy mildew although its fruits have reduced commercial quality. | RESUMO: O míldio, causado pelo fungo Pseudoperonospora cubensis é uma das principais doenças foliares que acometem a cultura do meloeiro no período chuvoso da região Nordeste brasileira. O objetivo do presente trabalho foi identificar e caracterizar morfologicamente fontes de resistência de meloeiro a P. cubensis. Trinta e seis acessos e quatro cultivares comerciais foram avaliados em delineamento em blocos casualizados com três repetições. A unidade experimental foi composta por sete plantas. E a avaliação realizada em condições de campo com uma escala diagramática, a qual fora calculado o índice de porcentagem da doença (IPD). As fontes de resistência e genótipos referências foram caracterizadas morfologicamente. Os acessos C-RN-2, C-SE-2, C-CE-2, C-PE-2, C-PI-1 e C-BA-2 apresentam resistência a P. cubensis e são promissores para uso em programas de melhoramento visando resistência ao míldio do meloeiro, embora os seus frutos tenham reduzida qualidade comercial.

The objective was to evaluate the growth of clones of forage cactus, subjected to different conditions of water availability in the Brazilian Semiarid, during a year of climate anomaly (biennium 2012-2013). The experiment was carried out in the first production year of the second crop cycle (March 2012 to February 2013), in the district of Serra Talhada, State of Pernambuco. The experiment was carried out in a randomized block design using a factorial arrangement 3x3 with three replications, being used three intervals of application of a fixed irrigation depth (7.5 mm), (7, 14 and 28 days), and three forage cactus clones (IPA: IPA Sertânia; MIU: Míuda and, OEM: Orelha de Elefante Mexicana). Eight biometric campaigns were performed during the experiment period for morphological analysis of the plants and cladodes along the time. It was found that water availability conditions showed no significant influence (P>0.05) on the majority of the absolute and relative values of the growth variables of the three forage cactus clones. However, when comparisons were performed among different clones, regardless of the water availability conditions, it was observed that, in terms of absolute values, the OEM had the highest means, differing only from the MIU clone. Regarding evaluations over time, there were increased growth rates in the last months of the crop cycle because of the occurrence of rainfall, which in conjunction with the application of the irrigation treatments promoted the best biometric increments for the OEM and IPA Sertânia clones.

RESUMO: Objetivou-se avaliar a evolução do crescimento de clones de palma forrageira submetidos a diferentes condições de disponibilidade de água no Semiárido brasileiro durante um ano de anomalia climática (biênio 2012-2013). O experimento foi conduzido no primeiro ano produtivo do segundo ciclo da cultura (março de 2012 a fevereiro de 2013) no município de Serra Talhada (PE). O delineamento experimental foi em esquema fatorial 3x3 com três repetições, sendo utilizados três intervalos de aplicação de uma lâmina fixa de 7,5 mm (7, 14 e 28 dias) e três clones de palma forrageira (IPA: IPA Sertânia; MIU: Miúda; e OEM: Orelha de Elefante Mexicana). Durante o experimento foram realizadas avaliações biométricas para análise morfológica da planta e dos cladódios ao longo do tempo. Constatou-se que as condições de disponibilidade de água não apresentaram influências significativas (P>0,05) sobre a maioria dos valores absolutos e relativos das variáveis de crescimento dos três clones. Já quando se compararam os diferentes clones, independentemente da condição de disponibilidade de água, observou-se que em termos de valores absolutos a OEM apresentou as maiores médias, diferindo apenas da MIU. Quanto a avaliação ao longo do tempo houve aumento das taxas de crescimento nos últimos meses do ciclo decorrente da ocorrência de eventos de precipitação pluviométrica, que em conjunto com a aplicação dos tratamentos de irrigação promoveram melhores incrementos biométricos nos clones OEM e IPA Sertânia. | ABSTRACT: The objective was to evaluate the growth of clones of forage cactus, subjected to different conditions of water availability in the Brazilian Semiarid, during a year of climate anomaly (biennium 2012-2013). The experiment was carried out in the first production year of the second crop cycle (March 2012 to February 2013), in the district of Serra Talhada, State of Pernambuco. The experiment was carried out in a randomized block design using a factorial arrangement 3x3 with three replications, being used three intervals of application of a fixed irrigation depth (7.5 mm), (7, 14 and 28 days), and three forage cactus clones (IPA: IPA Sertânia; MIU: Míuda and, OEM: Orelha de Elefante Mexicana). Eight biometric campaigns were performed during the experiment period for morphological analysis of the plants and cladodes along the time. It was found that water availability conditions showed no significant influence (P>0.05) on the majority of the absolute and relative values of the growth variables of the three forage cactus clones. However, when comparisons were performed among different clones, regardless of the water availability conditions, it was observed that, in terms of absolute values, the OEM had the highest means, differing only from the MIU clone. Regarding evaluations over time, there were increased growth rates in the last months of the crop cycle because of the occurrence of rainfall, which in conjunction with the application of the irrigation treatments promoted the best biometric increments for the OEM and IPA Sertânia clones.

This study aimed to evaluate the culture of Litopenaeus vannamei marine shrimp fed with dif- ferent protein levels diets in heterotrophic systems with and without probiotic addition. It was adopted a com- pletely randomized design with 4x2 factorial arrangement, using four dietary protein levels (20, 25, 30 e 35% CP), as the first factor (P20, P25, P30, and P35), and probiotic addition in the water, as the second factor (P20 Pro , P25 Pro P30 Pro e P35 Pro ). For this were used 24 fiberglass tanks (800 L working volume) stocked with 300 shrimp m - 3 (initial weight 1.55±0.01 g). Water quality parameters were analyzed periodically and showed no significant differences, except nitrite that was influenced by the protein levels ( P<0.05 ). After 50 culture days, shrimp final weight averaged 7.2±0.4 g ( P≥0.05 ). The interaction of protein levels vs. probiotic addition influenced significantly ( P<0.05 ) the survival (70.6 to 90.0%) and final biomass (1.3 - 2.0 Kg m - 3 ). In Litopenaeus vannamei intensive culture with the utilization of biofloc as the supplemental food, it is possible to reduce the protein levels of feed from 35 to 25%, without compromising the shrimp growth performance and water quality of the culture.

ABSTRACT: This study aimed to evaluate the culture of Litopenaeus vannamei marine shrimp fed with different protein levels diets in heterotrophic systems with and without probiotic addition. It was adopted a completely randomized design with 4x2 factorial arrangement, using four dietary protein levels (20, 25, 30 e 35% CP), as the first factor (P20, P25, P30, and P35), and probiotic addition in the water, as the second factor (P20Pro, P25Pro P30Pro e P35Pro). For this were used 24 fiberglass tanks (800 L working volume) stocked with 300 shrimp m-3 (initial weight 1.55±0.01 g). Water quality parameters were analyzed periodically and showed no significant differences, except nitrite that was influenced by the protein levels (P<0.05). After 50 culture days, shrimp final weight averaged 7.2±0.4 g (P≥0.05). The interaction of protein levels vs. probiotic addition influenced significantly (P<0.05) the survival (70.6 to 90.0%) and final biomass (1.3-2.0 Kg m-3). In Litopenaeus vannamei intensive culture with the utilization of biofloc as the supplemental food, it is possible to reduce the protein levels of feed from 35 to 25%, without compromising the shrimp growth performance and water quality of the culture. | RESUMO: O presente trabalho avaliou o desempenho do camarão marinho Litopenaeus vannamei alimentado com dietas de diferentes níveis protéicos em sistema de bioflocos com e sem a adição de probiótico. Foi adotado um delineamento experimental inteiramente casualizado com arranjo fatorial 4×2, com quatro níveis de proteína na dieta (20, 25, 30 e 35% PB), como primeiro fator (P20, P25, P30 e P35), e a adição de probiótico na água de cultivo, como segundo fator (P20Pro, P25Pro, P30Pro e P35Pro). Foram utilizados 24 tanques em fibra de vidro (800 L volume útil) estocados com 300 camarões m-3 (peso inicial 1,55±0,01 g). As variáveis de qualidade da água foram mensuradas periodicamente e não apresentaram diferença estatística, exceto o teor de nitrito, influenciado significativamente (P<0,05) pelos níveis de proteína. Após 50 dias de cultivo o peso médio final dos camarões foi de 7,2±0,4 g (P≥0,05) entre os tratamentos. A interação entre os níveis protéicos e a adição de probiótico influenciaram significativamente (P<0,05) na sobrevivência (70,5-90,0%) e na biomassa final (1,3-2,0 Kg m-3). Dessa forma, em cultivo intensivo de L. vannamei, com utilização de bioflocos como fonte de alimento suplementar, é possível reduzir os níveis de proteína da ração de 35 para 25% sem comprometer o desempenho zootécnico dos camarões e a qualidade da água.