The use of wastewater has emerged as alternative to control environmental pollution and a viable option to improved water availability in the arid and semiarid zones. In this study, the effects of irrigation solution with domestic sewage effluent and, of growing substrate on growth of Aroeira seedlings was examined in a greenhouse experiment. The effects of five irrigation solution (Public-supply water, PSW, domestic sewage effluent, DSE and the mixtures of 75% DSE + 25% PSW, 50% DSE + 50% PSW and 25% DSE + 75% PSW) and two growing substrate (75% soil + 25% bovine manure and, 75% soil + 25% coconut fiber) were used in a completely randomized block design, arranged as split plots with three replications for each treatment. The evaluations were made at 30, 60, 90 and 150 days after planting, and the parameters following were measured: stem diameter, shoot height, relationship between height and diameter, shoot/root dry matter ratio, dry matter of the root, shoot and total and, Dickson index. The analysis results indicated that the irrigation solution composed by raw sewage effluent or mixed with Public-supply water increased the growth and improved morphological index of Aroeira seedlings, being the better development found when plant of Aroeira were grown in bovine manure + soil substrate under irrigation with solution containing 100% domestic sewage effluent.

Green manure may lead to physical, chemical, and biological improvements to the soil. However, the information on its use in the Brazilian Northeast is scarce. The aim of this study was to assess the phenological cycle and phytomass productivity of seven legume species. This study was conducted in the second semester of 2015 in a completely randomized design with seven treatments and four replications. Plant height, fresh mass of shoot (FMS), dry mass of shoot (DMS), fresh mass of root (FMR), and dry mass of root (DMR) were determined at 100 days after sowing. Germination, flowering, and pod maturation were also assessed. The highest averages of FMS were observed in Crotalaria breviflora and Crotalaria mucronata, followed by Canavalia ensiformis, whereas the highest averages of DMS were observed in C. breviflora, C. ensiformis, C. mucronata, and Cajanus cajan cv. IAPAR 43. The highest averages of FMR were obtained by C. breviflora and C. mucronata, followed by C. cajan cv. Fava Larga. The species C. mucronata, C. cajan cv. IAPAR 43, and C. breviflora presented the highest averages of DMR. In addition, C. juncea, C. ensiformis, and C. cajan cv. IAPAR 43 were the earlier species regarding flowering, while C. cajan cv. Fava Larga was the later species. C. juncea was the earliest among all studied species regarding pod maturation. The tested legume species obtained promising initial results during the assessment period, being able to meet the expectations of biomass production and contributing to soil sustainability in this region.

Myroxylon balsamum is a valuable tropical tree; besides the good quality of its timber it also provides valuable nontimber forest products: balsam and resin. High deforestation rates in the area of distribution and the unregulated extraction of its resin and wood have led M. balsamum to be endangered or threatened. Its regeneration through seeds has been poorly studied, and there are no reports of germination and plasticity under controlled conditions. We evaluated the effect of scarification (longitudinal cut of the fruit and embryo fully exposed) and environmental factors (different levels of light intensities and changing temperatures) on seed germination and the development of seedlings. From the aforementioned, M. balsamum seeds are orthodox and show physical dormancy. In view of these characteristics, M. balsamum can be managed efficiently to initiate conservation programs and restoration in degraded land.

ABSTRACT The interaction Fusarium oxysporum f. sp. cubense (Foc) x nematode and the genetic variability of Foc are the main problems with potential to affect the use of resistant varieties in the management of the Panama disease. The objective of this work was to evaluate the interaction between Foc and Meloidogyne javanica on the banana of the Prata Anã, Grande Naine and BRS Princesa cultivars. The experiment was conducted in a randomized block design, with six treatments and ten replications, which differed in the inoculation time of each pathogen. Simultaneous inoculation with Foc and M. javanica, inoculation with Foc one week before inoculation with M. javanica, and inoculation with M. javanica one week before inoculation with Foc. In addition to the controls, Foc isolated, M. javanica isolated, and cultivars without the pathogens. The seedlings were transplanted in 3-liter pots with sterile soil infested with 40 grams of Foc inoculum at the concentration of 1x106CFU/gram and 1000 eggs and J2 of M. javanica. At the end of 56 days, the treatments with 'Grande Naine' presented the highest reproduction factors of M. javanica. In the 'Prata Anã' no interaction was observed between Foc x M. javanica. The treatments with simultaneous inoculation of the two pathogens, and with Foc a week before, in the 'BRS Princesa', presented the highest external disease indices (DI), which promoted the highest AUDPC. The highest internal DI were observed in the treatments Foc+Mj+Grande Naine, Foc+Mj+BRS Princesa, Mj+Grande Naine+Foc and Foc+BRS Princesa+Mj. The simultaneous presence of M. javanica and Foc increases the severity of the Panama disease in Grande Naine and BRS Princesa cultivars. | RESUMO A interação Fusarium oxysporum f. sp. cubense (Foc) x nematoide e a variabilidade genética de Foc são os principais problemas com potencial de afetar o uso de variedades resistentes no manejo do mal-do-Panamá. O objetivo do trabalho foi avaliar a interação entre Foc e Meloidogyne javanica sobre as cultivares de bananeiras, Prata Anã, Grande Naine e BRS Princesa. O experimento foi conduzido em DBC, com seis tratamentos e dez repetições, os quais se diferenciaram no tempo de inoculação de cada patógeno. Inoculação simultânea de Foc e M. javanica, inoculação de Foc uma semana antes de M. javanica e inoculação de M. javanica uma semana antes de Foc. Além dos controles, Foc isolado, M. javanica isolado e cultivares sem patógenos. As mudas foram transplantadas em vasos de três litros com solo estéril infestado com 40 gramas de inóculo de Foc na concentração de 1x106UFC/grama e 1000 ovos e J2 de M. javanica. Ao final de 56 dias, os tratamentos em 'Grande Naine' apresentaram os maiores fatores de reprodução de M. javanica. Em 'Prata Anã' não foi observado interação entre Foc x M. javanica. Os tratamentos com inoculação simultânea dos dois patógenos e com Foc uma semana antes, em 'BRS Princesa', apresentaram os maiores índices de doença (ID) externos, o que proporcionou as maiores AACPD. Os maiores ID internos foram observados nos tratamentos, Foc+Mj+Grande Naine, Foc+Mj+BRS Princesa, Mj+Grande Naine+Foc e Foc+BRS Princesa+Mj. A presença conjunta de M. javanica e Foc aumenta a severidade do mal-do-Panamá nas cultivares Grande Naine e BRS Princesa.

ABSTRACT Green manure may lead to physical, chemical, and biological improvements to the soil. However, the information on its use in the Brazilian Northeast is scarce. The aim of this study was to assess the phenological cycle and phytomass productivity of seven legume species. This study was conducted in the second semester of 2015 in a completely randomized design with seven treatments and four replications. Plant height, fresh mass of shoot (FMS), dry mass of shoot (DMS), fresh mass of root (FMR), and dry mass of root (DMR) were determined at 100 days after sowing. Germination, flowering, and pod maturation were also assessed. The highest averages of FMS were observed in Crotalaria breviflora and Crotalaria mucronata, followed by Canavalia ensiformis, whereas the highest averages of DMS were observed in C. breviflora, C. ensiformis, C. mucronata, and Cajanus cajan cv. IAPAR 43. The highest averages of FMR were obtained by C. breviflora and C. mucronata, followed by C. cajan cv. Fava Larga. The species C. mucronata, C. cajan cv. IAPAR 43, and C. breviflora presented the highest averages of DMR. In addition, C. juncea, C. ensiformis, and C. cajan cv. IAPAR 43 were the earlier species regarding flowering, while C. cajan cv. Fava Larga was the later species. C. juncea was the earliest among all studied species regarding pod maturation. The tested legume species obtained promising initial results during the assessment period, being able to meet the expectations of biomass production and contributing to soil sustainability in this region. | RESUMO A adubação verde pode atribuir melhorias físicas, químicas e biológicas aos solos, porém, são escassas as informações de uso no nordeste brasileiro. Objetivou-se avaliar o ciclo fenológico e as produtividades de fitomassa de sete espécies de leguminosas. O trabalho foi conduzido durante o segundo semestre de 2015, em delineamento inteiramente casualizado, com sete tratamentos e quatro repetições. Aos 100 dias após semeadura (DAS) foram determinadas a altura das plantas, massa fresca (MFPA) e seca da parte aérea (MSPA), massa fresca (MFR) e seca da raiz (MSR). Avaliou-se também a germinação, florescimento e maturação das vagens. As maiores médias para MFPA foram obtidas pelas Crotalaria breviflora e Crotalaria mucronata, seguidas da Canavalia ensiformis. As mais produtivas para MSPA foram C. breviflora, C. ensiformis, C. mucronata, e Cajanus cajan Cv IAPAR 43. As maiores médias para MFR foram obtidas pelas C. breviflora e C. mucronata, seguidas do C. cajan Cv Fava Larga. A C. mucronata, C. cajan Cv IAPAR 43 e C. breviflora foram as espécies que obtiveram as maiores médias de MSR. A Crotalaria juncea, C. ensiformis e C. cajan Cv IAPAR 43 foram as espécies mais precoces em relação à floração, enquanto que C. cajan Cv Fava Larga foi a espécie mais tardia. C. juncea foi a mais precoce, entre todas as espécies estudadas, para a variável maturação das vagens. As espécies de leguminosas testadas obtiveram, durante o período de avaliação, resultados iniciais promissores, podendo atender as expectativas de produção de biomassa, além de contribuir para a sustentabilidade dos solos desta região.

ABSTRACT The use of wastewater has emerged as alternative to control environmental pollution and a viable option to improved water availability in the arid and semiarid zones. In this study, the effects of irrigation solution with domestic sewage effluent and, of growing substrate on growth of Aroeira seedlings was examined in a greenhouse experiment. The effects of five irrigation solution (Public-supply water, PSW, domestic sewage effluent, DSE and the mixtures of 75% DSE + 25% PSW, 50% DSE + 50% PSW and 25% DSE + 75% PSW) and two growing substrate (75% soil + 25% bovine manure and, 75% soil + 25% coconut fiber) were used in a completely randomized block design, arranged as split plots with three replications for each treatment. The evaluations were made at 30, 60, 90 and 150 days after planting, and the parameters following were measured: stem diameter, shoot height, relationship between height and diameter, shoot/root dry matter ratio, dry matter of the root, shoot and total and, Dickson index. The analysis results indicated that the irrigation solution composed by raw sewage effluent or mixed with Public-supply water increased the growth and improved morphological index of Aroeira seedlings, being the better development found when plant of Aroeira were grown in bovine manure + soil substrate under irrigation with solution containing 100% domestic sewage effluent. | RESUMO O uso das águas residuais tem despontado como uma alternativa para o controle da poluição ambiental e uma opção viável para aumentar a disponibilidade hídrica nas regiões áridas e semiáridas. Neste estudo, os efeitos da água de irrigação com efluente de esgoto doméstico e do substrato de cultivo sob o crescimento de mudas de Aroeira foram estudados em um experimento em condições de ambiente protegido. Foram testados cinco soluções de irrigação (Água de abastecimento - AA, Efluente de esgoto doméstico bruto - ED e as misturas de 75% ED + 25% AA, 50% ED + 50% AA e 25% ED + 75% AA) e dois substratos de cultivos (75% solo + 25% esterco de bovinos e 75% solo + 25% fibra de coco) utilizando o delineamento estatístico inteiramente casualizado, arranjadas em parcelas subdivididas com três repetições com três repetições por tratamento. As avaliações de crescimento e desenvolvimento foram realizadas aos 30; 60; 90 e 150 dias após o plantio e determinaram-se as variáveis diâmetro do colo, altura de parte aérea, relação entre altura e o diâmetro, relação matéria seca da parte aérea/matéria seca da raiz, matérias secas do sistema radicular, da parte aérea, total, e o índice de Dickson. As análises dos resultados indicaram que a irrigação com efluente de esgoto aumentou o crescimento e o índice morfológico das mudas de aroeira, sendo o melhor desenvolvimento encontrado quando as plântulas de Aroeira foram cultivadas em substrato de esterco bovino + solo e irrigadas com solução contendo 100% efluente de esgoto doméstico tratado.

RESUMO O uso de estimulantes para o estabelecimento de fungos micorrízicos arbusculares tem grande potencial em contribuir para a absorção de P pelas plantas. Objetivou-se avaliar a eficácia do isoflavonóide formononetina como estimulante da micorrização na cultura da soja, associada a adubação fosfatada, em dois Latossolos com teores de fósforo disponível médio (LPm) e baixo (LPb) no Cerrado piauiense. O delineamento experimental em cada solo foi em blocos casualizados no esquema fatorial 4×4, com quatro repetições. Os tratamentos foram constituídos por quatro doses de fósforo (0; 26,66; 40 e 80 kg ha-1 de P2O5) e quatro doses do isoflavonóide formononetina (0; 0,5; 0,9 e 1,8 g kg-1 de semente de soja). As variáveis avaliadas foram, o crescimento, a produção, a nodulação e taxa de colonização micorrízica da soja. Com exceção da taxa de colonização micorrízica no LPm, altura e número de vagens por planta de soja no LPb, as demais variáveis, não foram influenciados pela aplicação do isoflavonóide formononetina, em ambos os solos. A produtividade cresceu linearmente ao incremento das doses de fósforo no LPb, apresentando uma eficiência agronômica de ~15 kg ha-1 de grãos para cada kg ha-1 de P2O5 aplicado no solo. | ABSTRACT The use of stimulants in the establishment of arbuscular mycorrhizal fungi has great potential in contributing to P uptake by plants. This study aimed to evaluate the effect of isoflavonoid formononetin as mycorrhization stimulant on soybean associated with phosphate fertilizer in Oxisols with intermediate (OPi) and low (OPl) phosphorus availability, in the Cerrado region of Piauí. The experiment in each soil consisted of randomized blocks design in a 4×4 factorial scheme, with four replications. The treatments comprised of four phosphorus doses (0, 26.66, 40 and 80 kg ha-1 P2O5), and four isoflavonoid formononetin doses (0, 0.5, 0.9 and 1.8 g kg-1 soybean seeds). The variables evaluated were soybean growth, yield, nodulation and mycorrhizal colonization rate. With the exception of mycorrhizal colonization rate in OPi, plant height and number of pods per plant in OPl, the other variables were not affected by the application of isoflavonoid formononetin, in both soils. Yield linearly increased with increase in the phosphorus doses in OPl, and presented agronomic efficiency of ~15 kg ha-1 grain for each kg ha-1 P2O5 applied to the soil.

ABSTRACT The study was conducted in irrigated commercial orchards of ‘Paluma’ and ‘Pedro Sato’ guavas, which were mapped with a Garmin GPS unit (Cx60). Sixty five sampling points were marked on a 21 x 21 m sampling grid. Gravimetric humidity and soil penetration resistance (SPR) were evaluated with the help of an automated penetrometer rod with a type 3 cone at a 45º angle (maximum SPR 15,100 kPa). Data with non-normal distribution were analyzed by geostatistics and interpolation by ordinary kriging. SPR values were higher where machine traffic occurs than on the lines where the crop is planted. There was spatial variability of the SPR among the layers evaluated, with the layer between 0.10-0.20 m, on the line of the machine traffic, presenting a higher SPR (>4000 kPa). | RESUMO O estudo foi conduzido em pomares comerciais de goiabeiras ‘Paluma’ e ‘Pedro Sato’, irrigados, que foram mapeados com GPS de navegação Garmin (Cx60). Houve a demarcação de 65 pontos de amostragem, demarcados em grade amostral de 21 x 21 m, sendo avaliadas a umidade gravimétrica e a resistência do solo à penetração (RSP), com auxílio de um penetrômetro automatizado, haste com cone tipo 3 com ângulo de 45º (RSP máximo de 15.100kPa). Os dados com distribuição não normal foram analisados por geoestatística e interpolação por krigagem ordinária. Os valores de RSP, onde ocorre o tráfego de máquinas, foi maior do que o avaliado na linha de plantio da cultura. Houve variabilidade espacial da RSP entre as camadas avaliadas, sendo que a camada entre 0,10-0,20 m, na linha do tráfego de máquinas, apresentou a maior RSP (>4000kPa).

ABSTRACT The aim of this study was to apply optical microscopy and scanning electron microscopy (SEM) techniques to identify and assess dripper clogging of an irrigation system. Three dripper models (Taldrip, Streamline, and Tiran) underwent 1200 hours of irrigation with three types of effluents: treated domestic wastewater, water with a high calcium content, and urban water supply. Samples of the material adhered to the inside of drippers were analyzed by means of the smear method aiming at their biological identification. Other samples were taken to obtain SEM images. The presence of algae, bacteria, and biological components of biofilm was observed in drippers operating with wastewater and water with calcium, this latter in a low number. The formation of biofilm and chemical precipitates of calcium were observed in SEM images, leading to emitter clogging. Optical microscopy and SEM analyses allowed identifying the degree of fouling by biofilm and chemical precipitates in dripper clogging. | RESUMO O objetivo da pesquisa foi aplicar as técnicas de microscopia óptica e Microscopia Eletrônica de Varredura (MEV) para identificar e avaliar os causadores da obstrução dos gotejadores de irrigação localizada. Três modelos de gotejadores (Taldrip, Streamline e Tiran) foram submetidos a 1200 horas de irrigação com três tipos de efluentes: água residuária de esgoto doméstico tratado água com elevado teor de cálcio e água de abastecimento urbano. Amostras do material aderida no interior dos gotejadores foram analisadas pelo método de esfregaço para fazer a identificação biológica, outras amostras foram retiradas para realizar as imagens com MEV. Foi possível verificar a presença de algas, bactérias e componentes biológicos do biofilme, tanto nos gotejadores operando com água residuária, quanto naqueles que operavam com água com cálcio, esta última em menor quantidade. Nas imagens com MEV foi possível verificar a formação de biofilme que causou a obstrução dos emissores e também os precipitados químicos de cálcio. A análise por MEV e microscopia óptica permitiu identificar o grau de incrustação por biofilme e precipitados químicos na obstrução de gotejadores.

RESUMO O feijão comum (Phaseolus vulgaris L.) é uma das fontes proteicas mais importantes em todo o mundo. Todavia, o ataque de bruquídeos inviabiliza o armazenamento do produto após a colheita. O objetivo deste trabalho foi determinar a taxa de emergência de Zabrotes subfasciatus (Coleoptera: Chrysomelidae) em quatro variedades de feijão P. vulgaris (Carioca Pitoco, Enxofre, Gorgutuba Vermelho e Rosinha) cultivadas na Amazônia sul-ocidental (Acre, Brasil). Os grãos de cada variedade foram infestados com 50 adultos não-sexados e após 13 dias do início dos bioensaios, os insetos foram removidos. A progênie adulta obtida nas variedades foi contabilizada e removida em dias alternados, a partir da primeira emergência, até o final do período de emergência (n° de insetos/frasco). A soma da emergência de insetos foi acumulada a partir da emergência inicial e resultou na soma da emergência acumulada (SEa, % dia), calculada por SEa = ∑SEd. Foram constadas diferenças nas taxas de emergência de Z. subfasciatus entre as variedades de feijão, sendo que a variedade Gorgutuba Vermelho apresentou menor taxa de emergência. Embora esta variedade de feijão tenha apresentado menor emergência de adultos, o tempo de desenvolvimento dos insetos não se estendeu nesta variedade, em comparação com as outras variedades. Em geral, a variedade Gorgutuba Vermelho apresentou indícios de fontes resistência a Z. subfasciatus. | ABSTRACT Common beans (Phaseolus vulgaris L.) are one of the most important protein sources worldwide. However, infestation by bruchids compromises the storage of these beans after harvest. The objective of this study was to determine the emergence rate of Zabrotes subfasciatus (Coleoptera: Chrysomelidae) in four P. vulgaris varieties (Carioca Pitoco, Enxofre, Gorgutuba Vermelho, and Rosinha) cultivated in the southwestern Amazon (State of Acre, Brazil). The grains from each variety were infested with 50 non-sexed adult Z. subfasciatus, and the insects were collected 13 days after initiating the bioassays. The adult progeny was collected from each plant variety on alternate days from the beginning to the end of the emergence period, and they were counted (number of insects/jar). The sum of emerged insects per day (SEd) was determined from the beginning of the emergence period, from which the accumulated emergence was calculated (SEa= ΣSEd, % day). There were differences in the emergence rates of Z. subfasciatus among the bean varieties, with the rates being lower in the Gorgutuba Vermelho variety. Although this bean variety had the lowest peak of adult insect emergence, the period of insect development was not of increased length in this variety compared to the other varieties. In general, the Gorgutuba Vermelho variety appears to be resistant to Z. subfasciatus.