Characterizing genetic diversity based on evaluations involving germination and seed vigor, is a fundamental stage in starting the development of a cultivation system for Calotropis procera. Thus, this study aimed to estimate genetic diversity among 35 C. procera genotypes based on germination and vigor of seeds stored for 1 year. The genotypes, which originated from areas in Paraíba (Brazil), where they occur naturally, were collected and stored in March 2014. In order to evaluate the physiological potential of the seeds, two experiments were conducted between March and April 2015. The first involved a propagation test conducted in a germination chamber, while the second involved an emergence test in washed sand, performed in polyethylene trays in a screened environment under uncontrolled conditions. In both experiments, an entirely randomized design was used, with four repetitions of 25 seeds per genotype. The characteristics evaluated were germination, average daily germination, daily germination speed, the speed of germination index, the plantlet vigor index, emergence, the speed of emergence index, total length, and total dry mass. The results showed that genetic variability exists among the C. procera genotypes for variables involving germination and vigor of seeds stored for 1 year. Also, emergence, total dry plantlet mass, and germination characteristics contribute most to the genetic divergence among C. procera genotypes.

Brazil is one of the world's largest producers of melon (Cucumis melo L.), and Rio Grande do Norte and Ceará are the largest producers states of the country (99% of exports). This crop had great socio- economic importance in the Brazilian Northeast, however, it is affected by insect pests and consequently, large amounts of pesticides are applied to it, which greatly affect beneficial organisms, such as Chrysopidae. This bioassay evaluated the toxicity of nine insecticides used in commercial crops of muskmelon, applied to first- instar larvae of Chrysoperla genanigra of up to 24-hour-old, from mass rearing cultures. Sublethal effects were evaluated, classifying the insecticides into the toxicity classes recommended by the IOBC. A completely randomized design was used, consisting of ten treatments (clothianidin, pymetrozine, lambda-cyhalothrin, chlorantraniliprole, indoxacarb, pyriproxyfen, beta-cyfluthrin+imidacloprid, imidacloprid, beta-cypermethrin and a control consisted of distilled water). The treatments consisted of exposure of thirty larvae to dry residues of each product in Petri dishes, assessing their mortality, duration of instars, sex ratio, fecundity and viability of eggs from adults of the insects evaluated. The products were classified in toxicity classes as harmful (Class 4) (clothianidin, pymetrozine, indoxacarb, lambda-cyhalothrin, beta-cyfluthrin+imidacloprid, imidacloprid, beta- cypermethrin and pyriproxyfen) and innocuous (Class 1) (chlorantraniliprole) to first -instar larvae of C. genanigra, by calculate their total effect. Based on this work, chlorantraniliprole is the only recommended insecticide for use in integrated pest management (IPM) programs in muskmelon crops.

The aim of this study was to identify parents and promising hybrid combinations for the improvement of forage watermelon. Five parents were evaluated: BGCIA 996 (1), BGCIA 997 (2), BGCIA 998 (3), BGCIA 228 (4), Jojoba (5) and ten F1 hybrids, which were obtained from balanced diallel crosses. The experimental design was in a complete randomized block, with three replications. The morphoagronomic and bromatological traits were evaluated. The highlights were the progenitors BGCIA 997, BGCIA 998, BGCIA 228 and Jojoba for protein content, fruit yield, in vitro digestibility of dry matter and number of seeds, respectively. The hybrid 1x4 stood out for fruit length, seed number, and ethereal extract. The hybrid 2x3 stood out for rind and pulp thickness while the hybrids 3x4, 3x5 and 4x5 had exceptional digestibility, protein content and fruit yield, respectively. The analysis of the standard deviation of the SCA estimates of both SD (Sij- Sik) and SD (Sij- Skl) indicated that no hybrids were found that had SCA estimates twice that of SD (Sij- Sik) or SD (Sij- Skl), except for the hybrid 1x4 for the number of seeds per fruit. These results suggest that the parents were more promising than the hybrids. Similarly, the GCA estimates favor the intrapopulation method, which will promote greater efficiency in selection for genetic gains.

Pastas are generally accepted all over the world, mainly because they are versatile, cheap and easy - to - prepare. They are not nutritionally balanced, since they provide mainly carbohydrates. As a result of this, it is important to use ingredients which could improve the nutritional deficiencies, without affecting the technological and sensorial characteristics. This study evaluated the effect of using wheat semolina and micronized corn pericarp (MCP), on the proximate composition, cooking quality and color of spaghetti type pasta. Spaghetti pasta was produced using wheat semolina with the incorporation of micronized corn pericarp, at levels of 0, 10, 20 and 30%. There were no significant differences (p > 0.05) between the formulated samples with regards to the contents of moisture and lipid, cooking time, weight gain and volume increase. As observed, supplementation with micronized corn pericarp presented significant difference on the contents of proteins, minerals, dietary fiber and solid soluble loss of the spaghetti pasta (p < 0.05). With increase in micronized corn pericarp concentration, the color difference became accentuated. The use of MCP appears to be viable, providing a nutritionally enriched product without further impairment on pasta quality.

This study aimed to evaluate the quality of minimally - processed cassava treated with antioxidants and a starch - based edible coating. Cassava roots were washed, cooled, immersed in cold water, peeled and then cut. Root pieces were then immersed in a chloride solution, centrifuged, and subsequently immersed in either a starch suspension (3%), a solution containing antioxidants (3% citric acid and 3% ascorbic acid), or in both the coating and antioxidant solutions. Coated root pieces were dried at 18 ± 2°C for 1 hour, then packaged into polypropylene bags (150 g per pack) and kept at 5 ± 2°C for 15 days, and assessed every 3 days. A completely randomized design was used in a 4 × 6 factorial consisting of the treatment (control, coating, antioxidant, or coating and antioxidant) and the storage period (0, 3 6, 9, 12 or 15 days), with three replicates in each group. The pH, blackened area and peroxidase and polyphenol oxidase activities of the cassava was reduced in treatments containing antioxidants and the scores of visual analysis and phenolic content were higher. Therefore, treatment with antioxidants was effective for reducing browning in minimally - processed cassava, retaining the quality of cassava pieces stored for 15 days at 5 ± 2°C. The combination of antioxidants and the edible coating showed no improvement compared to treatment with antioxidants alone.

Research on the performance of cover crops in crop systems of annual crops in the Brazilian state of Piauí contributes to increases in yield, greater efficiency of fertilizers and mitigation of environmental impacts. The aim of this study was to evaluate the performance of cover crops in terms of biomass production and the accumulation and release of nutrients during the crop season (November to April) in an oxisol in the Brazilian Cerrado in the state of Piauí that was submitted to different crop production systems including soybeans, maize and upland rice. The experiment was established during the 2010/11 and 2011/12 crop years in the rural area near the municipality of Bom Jesus, Piauí. The experimental design was a randomized block in a split-plot array. Different soil management systems (conventional and no-till) were evaluated in the main plots. Different crop production systems consisting of cover crops sown in the off-season (April to November), and annual crops sown during the crop season (November to April) were implemented in the subplots. The crop production systems that included Urochloa ruziziensis and Pennisetum glaucum overseeded on soybeans and Urochloa ruziziensis simultaneously intercropped with maize stood out in terms of biomass production and the accumulation and release of nutrients. Yields of maize and upland rice declined when sown under newly implemented no-till soil management. Keywords: Brachiaria. Millet. Crotalaria. No-till. Research on the performance of cover crops in crop systems of annual crops in the Brazilian state of Piauí contributes to increases in yield, greater efficiency of fertilizers and mitigation of environmental impacts. The aim of this study was to evaluate the performance of cover crops in terms of biomass production and the accumulation and release of nutrients during the crop season (November to April) in an oxisol in the Brazilian Cerrado in the state of Piauí that was submitted to different crop production systems including soybeans, maize and upland rice. The experiment was established during the 2010/11 and 2011/12 crop years in the rural area near the municipality of Bom Jesus, Piauí. The experimental design was a randomized block in a split-plot array. Different soil management systems (conventional and no-till) were evaluated in the main plots. Different crop production systems consisting of cover crops sown in the off-season (April to November), and annual crops sown during the crop season (November to April) were implemented in the subplots. The crop production systems that included Urochloa ruziziensis and Pennisetum glaucum overseeded on soybeans and Urochloa ruziziensis simultaneously intercropped with maize stood out in terms of biomass production and the accumulation and release of nutrients. Yields of maize and upland rice declined when sown under newly implemented no-till soil management.

Among the factors negatively impacting guava ( Psidium guajava ) crop in Brazil, one of the most important is the root - knot nematode, Meloidogyne enterolobii , which may cause considerable yield losses and even the cessation of guava cultivation in some areas. In addition to affecting guava, the pathogen has been reported as a parasite in various other crops, among them both oleraceous and ornamental crops, as well as in spontaneous vegetation. The aim of this study was to verify the occurrence of M. enterolobii in plants collected in guava orchards in different counties of Ceará state, identified through electrophoresis with the isoenzyme esterase, and to observe its infecting behavior into Meloidogyne differentiating plants . Fifty root samples from guava, tasselflower ( Emilia fosbergii ), and jurubeba ( Solanum paniculatum ), were collected in 13 counties from eight micro - regions in the state of Ceará. In all analyzed samples, only esterase phenotype M2 (Rm: 0.6; 0.9), characteristic of M. enterolobii , was detected, showing that the nematode is widespread in orchards throughout the state, where is affecting these fruit tree, and that it is also able to parasitizing plants of the spontaneous vegetation. Based on the results, this nematode currently constitutes a serious threat to guava plantations in Ceará, and effective control mechanisms are crucial to prevent the spread of this pathogen to other, still unaffected, areas.

The development of technologies to increase stand establishment efficiency and seed vigor, as well as to maximize profits, is of fundamental importance for the sustainability of agriculture. Products based on humic substances, such as humic acids, have been recently used in Brazilian agriculture to produce seedlings with high vigor, which positively influences the establishment of the initial stand. Thus, this study aimed to evaluate the effect of treatment of corn seeds with a commercial humic acid-based product, Humykos® (18% of humic acid content), on germination and seedling vigor. The treatments consisted of six doses (0, 100, 200, 300, 400, and 500 mL 100 kg-1 seed) of Humykos®, and the treated seeds were analyzed for germination and vigor (first count, emergence, emergence speed index, length, and dry mass of shoots and roots). Our results show that humic acid promotes greater seedling growth and increased shoot dry mass in corn; in addition, it has a positive influence on the emergence speed index up to a dose 158 mL 100 kg-1 seeds.

The objective of this work was to evaluate seeds of cowpea, cultivar BRS Tumucumaque, subjected to different water stresses induced by polyethylene glycol (PEG-6000) and mannitol and different salt stresses induced by NaCl and CaCl2 solutions. The water and salt stresses was evaluated in laboratory using completely randomized experimental designs in 2x6 factorial arrangements, with six osmotic potentials (0.0, -0.2, -0.4, -0.6, -0.8 and -1.0 MPa) and two stress inducers for each experiment, with four replications of 50 seeds. The percentage of germination, germination speed index, root and shoot lengths and seedling fresh weight were evaluated at the tenth day (end of the evaluation period). The simulated water stress showed effects on the cowpea seed germination and seedling initial growth by decreasing the rates of the variables evaluated, which had greater effect in treatments with PEG-6000. The salt stress induced by NaCl showed greater effect on the variables evaluated than that induced by CaCl2, due to the sodium toxicity in the salt chemical composition.

In Brazil, species of the genus Liriomyza are widely distributed and have economic importance as they cause damage to at least 14 plant families, especially Solanaceae, Cucurbitaceae, Asteraceae, and Fabaceae. Studies suggest existence of a species complex within this genus, based on the presence of morphological similarities among the species Liriomyza trifolii (Burgess), L. sativae Blanchard and L. huidobrensis (Blanchard). The present study aimed to use DNA barcoding to establish new distribution records of L. sativae in distinct regions in Brazil, determine intra- and inter-population genetic diversity, and reconstruct the phylogeny of Liriomyza species using the DNA barcode sequences. Identity values were between 97% and 99%, confirming that all the examined Brazilian populations belonged to the species L. sativae. Phylogenetic analyses indicated the presence of a single clade of L. sativae, composed of seven populations. Intra-population analysis on individuals of these populations indicated low levels of nucleotide and haplotype diversity. The haplotype network indicated presence of only 14 haplotypes distributed among the Brazilian populations. The genetic similarities shared by the Brazilian populations of L. sativae suggest that these populations are closely related. Genetic patterns observed among populations of L. sativae might be associated with bottleneck events or founder effect during establishment of this leafminer in Brazil.