Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses could be food or water contaminants; however, with few exceptions, most well-characterized foodborne or waterborne viral outbreaks are restricted to hepatitis A virus (HAV) and calicivirus, essentially norovirus (NoV). Target viruses for analytical methods include, in addition to NoV and HAV, hepatitis E virus (HEV), enteroviruses (e.g., poliovirus), adenovirus, rotavirus, astrovirus, and any other relevant virus likely to be transmitted by food or water. A survey of the currently available methods for detection of viruses in food and environmental matrices was conducted, gathering information on protocols for extraction of viruses from various matrices and on the various specific detection techniques for each virus type.

Molecular Microbial Diagnostic Methods: Pathways to Implementation in the Food and Water Industries attempts to address the shortage of guidance on implementation of molecular-based methods for routine diagnostic laboratories. What industry and analysts can expect from routine use of these methods is discussed and outlined. The book uncovers industry needs for the use of molecular methods by providing a brief history of water and food analysis for the pathogens of concern. It also describes the potential impact of current and cutting-edge molecular methods. It discusses the advantages of the implementation of molecular methods, describes information on when and how to use specific methods, and presents why one should utilize them for pathogen detection in the routine laboratory. The reference material in the book is also pertinent for anyone carrying out microbiological analysis at the research level and covers a wide spectrum of classical and cutting-edge methods.

Background: Perchlorate-induced natrium-iodide symporter (NIS) interference is a well-recognized thyroid disrupting mechanism. It is unclear, however, whether a chronic low-dose exposure to perchlorate delivered by food and drinks may cause thyroid dysfunction in the long term. Thus, the aim of this review was to overview and summarize literature results in order to clarify this issue. Methods: Authors searched PubMed/MEDLINE, Scopus, Web of Science, institutional websites and Google until April 2020 for relevant information about the fundamental mechanism of the thyroid NIS interference induced by orally consumed perchlorate compounds and its clinical consequences. Results: Food and drinking water should be considered relevant sources of perchlorate. Despite some controversies, cross-sectional studies demonstrated that perchlorate exposure affects thyroid hormone synthesis in infants, adolescents and adults, particularly in the case of underlying thyroid diseases and iodine insufficiency. An exaggerated exposure to perchlorate during pregnancy leads to a worse neurocognitive and behavioral development outcome in infants, regardless of maternal thyroid hormone levels. Discussion and conclusion: The effects of a chronic low-dose perchlorate exposure on thyroid homeostasis remain still unclear, leading to concerns especially for highly sensitive patients. Specific studies are needed to clarify this issue, aiming to better define strategies of detection and prevention.

The microbiological safety of food and the environment in which we live is currently an intensely discussed topic. Increasing production and the demand-driven global market exert pressure on ensuring sufficient high-quality food and safe drinking water. Compared to the past, increased attention in this area is now paid to important viral agents associated with food/water contaminations in both intensive research and routine diagnostics. This interest in viral agents has also increased in recent years due to the ongoing global pandemic caused by the novel coronavirus. Food- and water-borne viruses usually cause only mild and short-term diseases. The most common is gastroenteritis manifested by fever, vomiting and watery diarrhoea. However, in addition to mild febrile illness, these agents can also cause more serious conditions – respiratory infections, hepatitis, conjunctivitis, aseptic meningitis, myocarditis, encephalitis and paralysis. Globally, these diseases have significant economic impacts and are still among the leading causes of death in developing countries.This manuscript provides an overview of food- and water-borne viruses and technologies developed and currently used for their identification as causative agents. Methods for the detection of these pathogens represent an important tool for the assessment and mitigation of potential risks associated with the contamination of food and water resources. There is currently a wide range of possible approaches. Their use is differently targeted and their sensitivity, effectiveness and specificity also vary. In the case of a specific application, it is therefore necessary to choose the appropriate method, optimize it, and then verify its applicability and limits. The chosen method should be sufficiently robust, sensitive, specific and, if possible, also time and labor saving.

Identifying pathogens in food and drinking water has always been an important task. Escherichia coli (E. coli) is one of these pathogens found in food and water samples. Although there are several traditional microbiological analysis methods, the most advanced methods are based on biochemistry and molecular biology. New nanotechnology methods based on optical methods provide cheaper, more reliable, faster, and more sensitive platforms for detecting E. coli in a given sample. Various optical methods are available for the detection of E. coli. The most recently developed strategies to develop sensors for detecting E. coli are fluorescence, colorimetric, surface-enhanced Raman spectroscopy, surface plasmon resonance, localized surface plasmon resonance, and chemiluminescence. In addition, optical detection of E. coli in smartphone, paper-based, and portable devices are also considered. It has been shown that these optical nanobiosensors have high sensitivity and low detection limits for E. coli detection.

Two enzyme-linked immunosorbent assay (ELISA) kits were evaluated for their effectiveness in detecting and differentiating between Shiga toxin 1 and 2 (Stx1 and Stx2) produced by Shiga toxin-producing E. coli (STEC) inoculated into food and water samples. Each kit incorporated monoclonal antibodies previously determined to bind all known Stx1 or Stx2 subtypes with the exception of Stx2b. Four different sample types, including ground beef, Romaine lettuce, pond water, and pasteurized milk were inoculated with Stx1a-, Stx2a-, or Stx1a- and Stx2a-producing STEC strains, enriched using modified tryptic soy broth (containing mitomycin C) for 6, 16, and 22 h, and tested using the ELISA kits in the presence of a bacterial protein extraction reagent (B-PER™). The two Shiga toxin types were readily detected and distinguished for all tested sample types. There was good overall sensitivity, specificity, variance, and reproducibility for the two ELISA kits and they should prove useful for application in food testing.

Norovirus (NoV) is a major cause of acute non-bacterial gastroenteritis in all age groups worldwide. To detect NoV from foods, polyethylene glycol (PEG) precipitation or ultracentrifugation methods are generally used with reverse transcription (RT)-PCR. These methods need to use complicated procedures and varied buffers depending on the kinds of food matrices. In this study, we suggested a universal method to recover NoV in food and water samples as a prior step to real-time RT-PCR. As a NoV surrogate model, feline calicivirus (FCV) was used. FCV was artificially inoculated to samples, and then concentrated by the adsorption-elution method using negatively charged membrane filters. The detection limit was 4.3×10¹ PFU/250 mL for distilled water, 4.3×10² PFU/250 mL for environmental waters, and 4.3×10² PFU/15 g for lettuce and oyster. We were able to identify the possibility of one universal and time-saving method to detect NoV in food and water samples without modifications.

During the last decade, food, feed and environmental analysis using high-resolution mass spectrometry became increasingly popular. Recent accessibility and technological improvements of this system make it a potential tool for routine laboratory work. However, this kind of instrument is still often considered a research tool. The wide range of potential contaminants and residues that must be monitored, including pesticides, veterinary drugs and natural toxins, is steadily increasing. Thanks to full-scan analysis and the theoretically unlimited number of compounds that can be screened in a single analysis, high-resolution mass spectrometry is particularly well-suited for food, feed and water analysis. This review aims, through a series of relevant selected studies and developed methods dedicated to the different classes of contaminants and residues, to demonstrate that high-resolution mass spectrometry can reach detection levels in compliance with current legislation and is a versatile and appropriate tool for routine testing.