Materials, energy, and organisms from groundwater serve as resource subsidies to lotic systems. These subsidies influence food production and post-emergent fish growth and condition through nutrient inputs and water temperature changes. To test whether post-emergent fish grew faster in gaining sites, we grew hatchery post-emergent salmon in enclosures, sampled periphyton, benthic invertebrates, and wild salmon, and modeled fish growth across a gradient of groundwater – surface water exchange. Fish grew almost twice as fast in gaining (2.7%·day⁻¹) than in losing (1.5%·day⁻¹) sites. Fish from transient sites grew as much as gaining sites, but their condition was significantly lower (18.3% vs. 20.7%). Results suggest that groundwater – surface water exchange affects fish growth and energetic condition through direct and indirect pathways. Elevated nitrogen concentrations and consistently warmer water temperature in gaining sites have a strong effect on basal production with subsequent effects on invertebrate biomass, fish growth, and condition. Findings highlight the importance of groundwater – surface water exchange as a subsidy to rearing salmon and may inform strategies for restoring fish rearing habitat.

The Pacific lamprey is an iconic native fish of great importance to the ecosystem and indigenous cultures in the U.S. Pacific Northwest. Pacific lamprey populations have declined in abundance from historic levels, and conservation aquaculture has been proposed as a technique to restore these populations. The present research focused on expanding the culture methods for larval lamprey. The larvae filter feed and cohabitate with juvenile salmonids in the wild, therefore the effluent water from rearing salmonids may be a viable source of water and food for culturing lamprey. This approach could be a sustainable method for raising lamprey at existing salmonid hatcheries. A nine week trial investigated the effects of rearing in effluent water from salmonid culture on the growth, survival, proximate composition, and fatty acid profile of larval lamprey. This trial also explored the potential of this rearing strategy to improve the water quality by removing nutrients from the effluent. The trial included three treatments testing the use of the effluent from steelhead trout (Oncorhynchus mykiss), a conventional lamprey diet (control diet), or both combined as a means for rearing lamprey. A fourth treatment where lamprey were excluded served as a water quality control to test the effect of lamprey presence on effluent quality. Lamprey survival was not affected by treatment. Lamprey reared solely on the effluent waste matched the survival and growth of fish fed the conventional diet. Lamprey fed the combination treatment grew faster than the conventional diet fed fish. Whole body lipid levels were elevated in lamprey from the combination treatment relative to the conventional diet fed lamprey. Crude protein in the whole bodies of effluent fed lamprey was low compared to fish from either of the treatments where the conventional diet was fed. Lamprey offered the effluent nutrients were high in saturated fats relative to the control fed fish, which reflected the lipid profile of this diet. However, lamprey from the combination treatment were lower in long chain polyunsaturated fatty acids relative to the control or the effluent treatment lamprey. There was no measureable improvement in water quality due to the presence of lamprey, though lamprey were able to sequester approximately 1.3% of the effluent dry matter as lamprey biomass. Overall, it appears the larval stage of lamprey can be effectively reared on salmonid effluent, and this method provides superior growth when used in combination with a conventional lamprey diet.

Yamato Tachibana (Tachibana; Citrus tachibana) is an endemic fruit and represents one of the oldest citrus species in Japan; it is grown in the Mie Prefecture. It has been attracting attention for its cultural heritage and unique scent. To evaluate biological activities of Tachibana, we fed several parts of the Tachibana fruit (whole fruit, pulp [albedo and segment wall], and flavedo) to adult zebrafish and found that Tachibana increased body weight and plasma triglycerides besides increasing overall food intake. We then created a simple fluorescence-based feeding assay using dried rotifer sheets and larval zebrafish (6 days postfertilization) to screen the various extracts of Tachibana parts. We found that water extracts of Tachibana pulp increased feeding volume in zebrafish. Although citrus species are believed to prevent obesity and obesity-associated diseases in general, our findings showed that water extracts of Tachibana increase food intake in zebrafish and lead to an increase in body weight. We suggest that Tachibana might reverse appetite loss in lean populations and may prove beneficial in aiding fish cultivation.