Digital | El agua electrolizada (AE) es un agente antimicrobiano no térmico eficaz contra Salmonella spp, un patógeno perjudicial para la salud de niños, adultos y lactantes. A diferencia de los productos químicos potencialmente peligrosos como el cloro, que puede causar problemas en la salud del ser humano. Por lo tanto, el objetivo principal de este estudio fue evaluar la eficacia del agua electrolizada como agente antimicrobiano contra Salmonella spp en los huevos de gallinas de Santander. Para ello, se analizaron 90 muestras de huevos obtenidos de tres granjas de Santander, adquiridos comercialmente; se llevó a cabo el aislamiento e identificación de Salmonella spp, en los huevos de gallina comerciales siguiendo el estándar ISO 6579-1. Posteriormente, se realizó un estudio de sensibilidad para verificar la concentración efectiva de agua electrolizada para inhibir Salmonella spp. Finalmente, se comparó el efecto antimicrobiano del agua electrolizada con respecto al antimicrobiano actualmente utilizado en los huevos comerciales. De los resultados se obtuvieron 4 muestras con presencia de Salmonella spp, lo que representa un 4.44%, en el cual 3 fueron en la cáscara del huevo, con un 3.33% y 1 en la clara-yema con un 1.11% de prevalencia entre las granjas demoniadas A y C. Se observa que el agua electrolizada ácida (pH 3.4) tuvo un mayor efecto antimicrobiano en comparación con el agua electrolizada alcalina (pH 11) y el hipoclorito de sodio (NaClO). Este estudio demostró la capacidad inhibitoria del agua electrolizada acida, logrando reducir la concentración de Salmonella spp en un 4.09 Log UFC/mL, en contraste con el hipoclorito de sodio, que logró una reducción del 2.90 Log UFC/mL, concluyendo que se podría emplear el antimicrobiano (agua electrolizada ácida) como alternativo del hipoclorito de sodio, como producto amigable con el ambiente. | Electrolyzed water (EW) is a non-thermal antimicrobial agent effective against Salmonella spp, a pathogen harmful to the health of children, adults and infants. Unlike potentially hazardous chemicals such as chlorine, which can cause human health problems. Therefore, the main objective of this study was to evaluate the efficacy of electrolyzed water as an antimicrobial agent against Salmonella spp in eggs from hens in Santander. For this purpose, 90 samples of eggs obtained from three Santander farms, acquired commercially, were analyzed; isolation and identification of Salmonella spp, in commercial hen eggs was carried out following the ISO 6579-1 standard. Subsequently, a sensitivity study was carried out to verify the effective concentration of electrolyzed water to inhibit Salmonella spp. Finally, the antimicrobial effect of the electrolyzed water was compared with the antimicrobial currently used in commercial eggs. The results showed 4 samples with the presence of Salmonella spp, representing 4.44%, 3 of which were in the eggshell, with 3.33% and 1 in the yolk-white with a prevalence of 1.11% between the Demonized A and C farms. It is observed that acidic electrolyzed water (pH 3.4) had a higher antimicrobial effect compared to alkaline electrolyzed water (pH 11) and sodium hypochlorite (NaClO). This study demonstrated the inhibitory capacity of acid electrolyzed water, reducing the concentration of Salmonella spp by 4.09 Log CFU/mL, in contrast to sodium hypochlorite, which achieved a reduction of 2.90 Log CFU/mL, concluding that the antimicrobial (acid electrolyzed water) could be used as an alternative to sodium hypochlorite, as an environmentally friendly product. | Pregrado | Microbiólogo Industrial

The present research aimed at evaluating the influence of the water quality in the life cycle and attraction of Aedes aegypti (L.) females to oviposit using different sources of water (raw sewage, effluent of UASB reactor, effluent of polishing lagoon, effluent of anaerobic filter, rain water and de-chlorinated water). The immature development time and survivorship were evaluated on a daily basis in two distinct feeding systems (with and without food). The quality of the water was shown to affect the egg and larval stages, but not the pupal or the adult. In the absence of food, no development was observed in rain water and de-chlorinated water. Immature development was faster in water sources from raw sewage, although with the lowest survivorship (37.3%). Free-choice tests indicated that females preferred to lay most of their eggs on water collected from the effluent of a UASB reactor, achieving the highest oviposition activity index (OAI) of 0.57. In non-choice tests, females laid larger batches of eggs in water collected from anaerobic filters (204.8 eggs), with the lowest number of eggs being laid on de-chlorinated water (37.3 eggs). It can be concluded that A. aegypti does not demonstrate any particular preference to lay eggs on clean water. This has serious implications for developing strategies to manage populations of this important vector in urban areas as it was shown to lay eggs and successfully develop on several different sources of water.