The German Fruit Genebank (https://www.deutsche-genbank-obst.de/) is a decentralized network dedicated to coordinating various germplasm collections across Germany. The aim is to conserve and utilize the genetic resources of native fruit species, i.e. pear. For effective conservation, the investigation of authenticity of the genetic resources is essential. Between 2019 and 2023, two projects were performed on the pear collections of the German Fruit Genebank to characterize the cultivars pomologically and molecularly. Eight partners of the pear network of the German Fruit Genebank participated in the projects. First, at least two experts of the Fruit Orchard Agency Schlaraffenburg (Schlaraffenburger gGmbH, https://schlaraffenburger.de/) identify the pear cultivars pomologically and define the authentic cultivar name per tree and accession. Second, 17 simple sequence repeat (SSR) markers and seven reference genotypes in recommendation of the European Cooperative Programme for Plant Genetic Resources (ECPGR; http://www.ecpgr.cgiar.org/) Malus/Pyrus working group were used for genetic fingerprinting. The molecular analysis, including experimental work and statistical analysis for grouping the genetic profiles based on similarity, was performed by Microsynth ecogenics GmbH (Balgach, Switzerland). The pomological and molecular data was compiled and resulted in 1,838 complete samples. The most important data features per sample are the cultivar name, the authenticity expressed by the trueness-to-type criterion, the molecular group and the genetic profile. Lastly, the individual samples were collated into unique, synthetic genetic profiles per distinguished pear cultivars with the help of a computer program and manual review. Mutations and sports of cultivars cannot be distinguished molecularly, but only pomologically and are grouped within the origin cultivar as they carry the same molecular group but different cultivar names. In total, 420 pear cultivars with corresponding genetic fingerprints were identified and evaluated for their authenticity as part of the German Fruit Genebank as presented in the published dataset. Additionally, the genetic fingerprints were aligned to international datasets to assign the Pyrus UNiQue genotype code (PUNQ). To evaluate the certainty of the synthetic genetic profiles, a quality ratio was calculated per genetic locus and on average where the consensus across samples is reflected.

Intercropping allows for a decrease in nitrogen fertilisers, better weed management, an increase in pollinators and secure crop productivity. The wheat quality proved to be equal or higher than in the sole crop.

During the last decade, climate change has had a direct impact on several plant-related aspects, such as physiological processes, disease–pest frequency, yield, and the qualitative composition of fruit [...]

This study mainly evaluated the responses in growth performance of growing pigs to different energy systems and energy levels in diets. Subsequently, we compared the nutrient digestibility and digestible nutrient concentrations of each energy level diet. In experiment 1, a total of 144 growing pigs with an average initial body weight (BW) of 26.69 ± 7.39 kg were randomly allotted to six dietary treatments (four pigs/pen; six replicates/treatment) according to a 2 × 3 factorial arrangement resulting from two energy systems (metabolizable energy [ME] and net energy [NE]) and three energy levels (low [LE], recommended [C], and high energy [HE]). Pigs were fed the experimental diets for 6 weeks and were allowed free access to feed and water during the experimental period. In experiment 2, 12 growing pigs with an average initial BW of 27.0 ± 1.8 kg were randomly allotted to individual metabolism crates and fed the six diets in a replicated 6 × 6 Latin square design. The six dietary treatments were identical to those used in the growth trial. Pigs were fed their respective diets at 2.5 times the estimated energy requirement for maintenance per day, and this was divided into two equal meals provided twice per day during the experimental period. Differences in energy systems and energy levels had no significant effect on the growth performance or nutrient digestibility (except acid-hydrolyzed ether extract [AEE]) of growing pigs in the current study. However, the digestible concentrations of ether extract, AEE, and acid detergent fiber (g/kg dry matter [DM]) in diets significantly increased (p < 0.05) with increasing energy levels. Additionally, there was a tendency (p = 0.09) for an increase in the digestible crude protein content (g/kg DM) as the energy content of the diet increased. Consequently, differences in energy systems and levels did not affect the BW, average daily gain, and average daily feed intake of growing pigs. This implies that a higher variation in dietary energy levels may be required to significantly affect growth performance and nutrient digestibility when considering digestible nutrient concentrations.

Objective Three experiments were conducted to evaluate the effects of Saccharomyces yeast postbiotics (SYP) in feeds for sows on the growth of offspring (Exp. 1), for nursery pigs on their growth (Exp. 2), and for nursery and finishing pigs on their growth (Exp. 3). Methods Exp. 1 had 80 sows at breeding assigned to 4 groups with SYP at 0, 0.050, 0.175, and 0.500 g/kg. Offspring were fed a common diet for 126 d. Exp. 2 had 144 barrows at 8 kg body weight (BW) allotted to CON (no SYP); YPC (SYP at 0.175 g/kg; d 0 to 42); and YPD (SYP at 1.25, 0.75, and 0 g/kg; d 0 to 7, d 8 to 21, and d 22 to 42, respectively) with 8 pens/treatment (6 pigs/pen). Exp. 3 had 96 barrows at 8 kg BW allotted to CON (no SYP); YPN (SYP at 0.175 g/kg; d 0 to 42); YPF (SYP at 0.100 g/kg; d 43 to 119); and YPA (SYP at 0.175 and 0.100 g/kg; d 0 to 42 and d 43 to 119, respectively) with 8 pens/treatment (3 pigs/pen). Results In Exp. 1, increasing SYP increased (p<0.05, quadratic) the sow body score (maximum at 0.30% SYP), reduced (p<0.05, quadratic) the days-wean-to-estrus (minimum at 0.27% SYP), and increased (p<0.05) offspring BW at weaning and their average daily gain (ADG) and feed efficiency (G:F) at d 126. In Exp. 2, ADG, average daily feed intake (ADFI), and G:F of YPC were the greatest (p<0.05). The ADG and ADFI of YPD were greater (p<0.05) than CON. Fecal score of YPC and YPD was smaller (p<0.05) than CON. In Exp. 3, YPA had the greatest (p<0.05) ADG and YPN and YPF had greater (p<0.05) ADG than CON. Conclusion SYP enhanced sow performance, offspring growth, growth of nursery and growing pigs with the greater efficacy at 0.27 to 0.32 g/kg feed.

The area of impact zones of industrial contamination, oil and oil products spills, motor vehicle emissions, radioactive substances, as well as pesticides and mineral fertilizers application on agricultural lands of the Russian Federation is calculated. The share of the territory under consideration in the total area of agricultural lands of the country does not exceed 3%. Soils in the zone of contamination influence are considered to be potentially polluted and should be included in the monitoring program of agricultural land. Soils outside the zone of influence of pollutant dissemination are uncontaminated. Regular monitoring of contamination of these soils should not be carried out, which will reduce the costs of monitoring. More than 97% of the Russian agricultural land is represented by uncontaminated soils and is suitable for the production of bio products. Regular monitoring of contamination of these soils should not be conducted, which will reduce the cost of the survey, including sample collection, preparation and analysis. In view of the lack of soil contamination, the transition to the cultivation of bio products should be based on declarative approach. In this case, bio production should meet the requirements of special cultivation technologies and the product quality standards.

The presence, distribution, and morphology of calcium oxalate (CaC2O4, CaOx) crystals were observed in the stunted bodies of Actinidia arguta (A. arguta) vines, and their ability of microorganisms to degrade CaOx crystals was analyzed. Leaves, branches, and roots of stunted A. arguta vines were collected. In the roots, CaOx crystal bundles were distributed in the form of dotted lines. In the branches, CaOx crystal bundles were observed blocking and accumulating in the vessels. In the leaves, CaOx crystal bundles were observed in a net-like entanglement. Therefore, CaOx crystals present in the roots, branches, and leaves hinder the growth. A hydroponic cultivation with different calcium (Ca) concentrations showed that the growth of A. arguta was best at a Ca concentration of 1 cmol+/kg; at a Ca concentration of 2 cmol+/kg, the edges of the leaves began to dry out after 7 days; and at a Ca concentration of 4 cmol+/kg, the entire leaves died within 14 days. From this study, it was concluded that CaOx crystals hindered the growth of A. arguta, and the Acinetobacter calcoaceticus (A. calcoaceticus) strain was effective in degrading CaOx crystals. Therefore, the Ca concentration in the soil of the cultivation site should be managed at less than 2 cmol+/kg.

The presence, distribution, and morphology of calcium oxalate (CaC2O4, CaOx) crystals were observed in the stunted bodies of Actinidia arguta (A. arguta) vines, and their ability of microorganisms to degrade CaOx crystals was analyzed. Leaves, branches, and roots of stunted A. arguta vines were collected. In the roots, CaOx crystal bundles were distributed in the form of dotted lines. In the branches, CaOx crystal bundles were observed blocking and accumulating in the vessels. In the leaves, CaOx crystal bundles were observed in a net-like entanglement. Therefore, CaOx crystals present in the roots, branches, and leaves hinder the growth. A hydroponic cultivation with different calcium (Ca) concentrations showed that the growth of A. arguta was best at a Ca concentration of 1 cmol+/kg; at a Ca concentration of 2 cmol+/kg, the edges of the leaves began to dry out after 7 days; and at a Ca concentration of 4 cmol+/kg, the entire leaves died within 14 days. From this study, it was concluded that CaOx crystals hindered the growth of A. arguta, and the Acinetobacter calcoaceticus (A. calcoaceticus) strain was effective in degrading CaOx crystals. Therefore, the Ca concentration in the soil of the cultivation site should be managed at less than 2 cmol+/kg.

Intercropping allows for a decrease in nitrogen fertilisers, better weed management, an increase in pollinators and secure crop productivity. The wheat quality proved to be equal or higher than in the sole crop.