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Genetic diversity and population structure of 288 potato (Solanum tuberosum L.) germplasms revealed by SSR and AFLP markers 全文
2017
Jian WANG | Lu HOU | Ruo-yu WANG | Miao-miao HE | Qing-chang LIU
Genetic diversity and population structure of 288 potato (Solanum tuberosum L.) germplasms revealed by SSR and AFLP markers 全文
2017
Jian WANG | Lu HOU | Ruo-yu WANG | Miao-miao HE | Qing-chang LIU
Potato (Solanum tuberosum L.) is an important staple food and economic crop in many countries. China has led world potato production in recent years. To understand the genetic diversity of potato germplasms and to enrich the current gene pool for potato improvement, we made a global collection consisted of 288 potato germplasms from eight countries and the International Potato Center (CIP). Using SSR and AFLP techniques, we evaluated the genetic diversity and population structure of these 288 potato accessions. A total of 190 alleles on 20 SSR loci were detected and all of the SSR alleles were polymorphic among these potato germplasms with an average of 9.5 alleles per SSR locus ranging from 2 to 23. The effective number of alleles per locus (Ne*), Nei's genetic diversity (H*), and Shannon's information index (I*) was from (0.1709±0.3698) to (1.6166±0.3414), (0.076±0.1388) to (0.3812±0.1886), and (0.1324±0.1970) to (0.5347±0.1440), respectively, and the mean polymorphic information content (PIC) value was 0.7312. A total of 988 AFLP alleles were detected by 10 AFLP primer combinations with 983 polymorphic alleles, and 99.49% alleles was polymorphic with an average of 98.3 polymorphic alleles per primer combination ranging from 91 to 116. The values of Ne*, H* and I* were from (1.5162±0.311) to (1.6423±0.3278), (0.3114±0.145) to (0.3675±0.1121), and (0.4761±0.1792) to (0.547±0.1322), respectively, and the average PIC value was 0.9871. Bayesian analysis discriminated the accessions into seven subgroup and an admix group. The majority of accessions from CIP and China were assigned into SG1, SG5, SG6, SG7 and admix group. Accessions in SG3 were mainly from CIP and two small groups SG2 and SG4 were mainly from northeastern China. In general, the results obtained from Bayesian statistical analysis, cluster analysis and principal coordinate analysis consistently revealed the lack of geographical differentiation among country-wide collections, indicating germplasm introduction was common for the countries out of potato origin center. The polymorphic markers and the differentiate genetic lineages found in this study provide useful information for potato improvement and conservation programs.
显示更多 [+] 显示较少 [-]Genetic diversity and population structure of 288 potato (Solanum tuberosum L.) germplasms revealed by SSR and AFLP markers 全文
2017
Wang, Jian | Hou, Lu | WANG, Ruo-yu | HE, Miao-miao | LIU, Qing-chang
Potato (Solanum tuberosum L.) is an important staple food and economic crop in many countries. China has led world potato production in recent years. To understand the genetic diversity of potato germplasms and to enrich the current gene pool for potato improvement, we made a global collection consisted of 288 potato germplasms from eight countries and the International Potato Center (CIP). Using SSR and AFLP techniques, we evaluated the genetic diversity and population structure of these 288 potato accessions. A total of 190 alleles on 20 SSR loci were detected and all of the SSR alleles were polymorphic among these potato germplasms with an average of 9.5 alleles per SSR locus ranging from 2 to 23. The effective number of alleles per locus (Ne*), Nei's genetic diversity (H*), and Shannon's information index (I*) was from (0.1709±0.3698) to (1.6166±0.3414), (0.076±0.1388) to (0.3812±0.1886), and (0.1324±0.1970) to (0.5347±0.1440), respectively, and the mean polymorphic information content (PIC) value was 0.7312. A total of 988 AFLP alleles were detected by 10 AFLP primer combinations with 983 polymorphic alleles, and 99.49% alleles was polymorphic with an average of 98.3 polymorphic alleles per primer combination ranging from 91 to 116. The values of Ne*, H* and I* were from (1.5162±0.311) to (1.6423±0.3278), (0.3114±0.145) to (0.3675±0.1121), and (0.4761±0.1792) to (0.547±0.1322), respectively, and the average PIC value was 0.9871. Bayesian analysis discriminated the accessions into seven subgroup and an admix group. The majority of accessions from CIP and China were assigned into SG1, SG5, SG6, SG7 and admix group. Accessions in SG3 were mainly from CIP and two small groups SG2 and SG4 were mainly from northeastern China. In general, the results obtained from Bayesian statistical analysis, cluster analysis and principal coordinate analysis consistently revealed the lack of geographical differentiation among country-wide collections, indicating germplasm introduction was common for the countries out of potato origin center. The polymorphic markers and the differentiate genetic lineages found in this study provide useful information for potato improvement and conservation programs.
显示更多 [+] 显示较少 [-]Driving factors of agrobiodiversity: which characteristics influence intra-household potato diversity in the peruvian Andes? 全文
2017
stef de haan | s. luttringhaus | s. polreich | v. suarez | w. pradel
S. Luttringhaus et al., 'Driving factors of agrobiodiversity: which characteristics influence intra-household potato diversity in the peruvian Andes?', p.1, 2017 | Domesticated potatoes (Solanum spp.) originated in the Andes. Genetic hotspots of this important staple food exist throughout the mountain range. The conservation of potato diversity (and agrobiodiversity in general) is necessary to ensure functioning ecosystems and food security. Sufficient genetic material is the backbone of evolution and breeding. Despite agricultural modernisation (by e.g. introducing improved potato varieties), smallholder farmers continue to cultivate a large number of potato landraces. The initiative Chirapaq Nan promotes in-situ conservation of such potato landraces and monitors some of their hotspots across the Andes. So far, the initiative?s focus was cataloguing cultivated landraces and their genetic resources. Now, the better understanding of farmers? varietal choice is an additional aim. That is crucial to efficiently design and carry out in-situ projects. This study contributes to these efforts by assessing socioeconomic and environmental characteristics, which influence farming households? potato diversity. After literature review and expert interviews, eleven independent variables of the domains location, household assets, potato production and cultural factors were chosen. A binary dependent variable assigning farmers with up to three landrace varieties to the low potato diversity group and the spare to the high diversity group was created. Subsequently we ran a binary logistic regression model with household data from two Central Andean Peruvian provinces. The International Potato Center (CIP) collected these data in production year 2011/12. Results showed that four variables have a significant impact on intra-household potato diversity. These are the province in which farmers reside, their wealth (measured by cows per household), their level of technology adoption (number of improved varieties per household), and their participation in potato fairs. This study provides an overview of possible incentives and disincentives of potato diversity. Future research should apply a mix of quantitative and qualitative methods to better capture and analyse farmers? intrinsic value systems regarding agrobiodiversity and changing varietal preferences
显示更多 [+] 显示较少 [-]Assessment of genetic diversity and relatedness in the Latvian potato genetic resources collection by DArT genotyping 全文
2017
Rungis, D. E. | Voronova, A. | Kokina, A. | Veinberga, I. | Skrabule, I. | Rostoks, N.
Potato (Solanum tuberosum L.) has been cultivated in Latvia since the 17th century, and formal breeding programmes have been established since the start of the 20th century. The Latvian potato genetic resource collection consists of 83 accessions of Latvian origin, including landraces, old cultivars released starting from the 1930's, modern cultivars and breeding material. These are maintained in field and in vitro collections. Pedigree information about the potato cultivars is often limited, and the use of hybrids of local cultivars as parents is common in the Latvian potato breeding programme. Ninety-four Latvian potato varieties and breeding lines and some commonly used foreign accessions were genotyped with the potato DNA diversity array technology. Analysis of the Latvian potato genetic resources collection revealed that the amount of genetic diversity has increased in the modern cultivars in comparison with the old cultivars.
显示更多 [+] 显示较少 [-]Are We Getting Better at Using Wild Potato Species in Light of New Tools? 全文
2017
Bethke, Paul C. | Halterman, Dennis A. | Jansky, Shelley
Potato (Solanum tuberosum L.), mankind's third most consumed food crop, originated as an interspecific hybrid in the Andean highlands. More than 100 species closely related to potato exist throughout Central and South America. Potato was introduced to Europe, Asia, and North America in the 16th and 17th centuries, but most cultivars were destroyed by late blight epidemics in the mid‐19th century. Late blight resistance genes from the wild relative Solanum demissum Lindl. were subsequently introduced into cultivated potato. Thus, a precedent for the use of wild relatives in potato genetic improvement was established a century ago. Intensive efforts have been made to collect and maintain wild relatives of potato in public germplasm repositories. Genetic diversity in these species is high, and most are sexually compatible with cultivated potato. Perhaps more than any other vegetable crop, potato can benefit from the introgression of genes for valuable traits from wild relatives. However, a century of breeding has realized that potential on only a few notable occasions. Important examples include improved processing quality and resistance to viruses and nematodes. Substantial barriers prevent the facile incorporation of genetic material from wild relatives into potato cultivars. Overcoming these barriers may require an expansion of germplasm collections to include well‐characterized individuals as a complement to population‐based accessions. To be most useful for potato improvement, individual wild species plants will need to be cataloged by allelic composition, haplotype, biochemical properties, and physiological responses to stress. Effectively using the genetic diversity in wild relatives is likely to rely heavily on alternative methods of potato breeding, augmenting reassortment, and selection at the tetraploid level with diploid breeding and genetic modification using biotechnology.
显示更多 [+] 显示较少 [-]Genetic Diversity and Core Collection for Potato (Solanum tuberosum L.) Cultivars from Cameroon as Revealed by SSR Markers 全文
2017
Anoumaa, Mariette | Yao, Nasser Kouadio | Kouam, Eric Bertrand | Kanmegne, Gabriel | Machuka, Eunice | Osama, Sarah Karen | Nzuki, Inosters | Kamga, Yanick Borel | Fonkou, Théophile | Omokolo, Dénis Ndoumou
Twelve pairs of SSR markers were used to evaluate the genetic diversity of 138 accessions of potato cultivars from the Western Highlands region of Cameroon. The average Polymorphism information content (PIC) value (0.74) and number of alleles (7.08) indicated a high genetic diversity of the potato cultivars tested. Analysis of molecular variance revealed that most of the genetic variations are found within geographic region (≥ 91%), resulting in high gene flow (Nm > 4 individuals). Local varieties had significantly more alleles than exotic varieties. Genetic diversity estimates for accessions from low elevations were significantly lower than those from medium and high elevations. Cluster analysis showed three clusters; the model-based approach inferred two gene pools. Genotypes revealed a high level of admixture between gene pools within locations and elevations. A core collection identified in this study composed of 27 individuals representing 19.57% of the whole collection and captured 99.15% of the total alleles found.
显示更多 [+] 显示较少 [-]Germplasm evaluation and molecular selection of potato (Solanum tuberosum L.) cultivars with disease resistance in China 全文
2017
Wan Lin LI | Wen GUO | Ji Ping XIAO | Lei BAI | Hua Chun GUO
Germplasm evaluation and molecular selection of potato (Solanum tuberosum L.) cultivars with disease resistance in China 全文
2017
Wan Lin LI | Wen GUO | Ji Ping XIAO | Lei BAI | Hua Chun GUO
Foreground and background selections are two important aspects that need to be carefully considered by plant breeders during field selection. In this article, we used 7 disease resistance markers, including four late blight and three potato virus disease resistance gene markers, and 12 microsatellite markers to evaluate the disease resistance and genetic diversity of 76 potato cultivars in total which were collected from 15 provinces of China. The foreground selection results showed that a number of materials, clustered separately, contained more than two late blight resistance markers or pyramided late blight and virus disease resistance gene markers together. A lot of them were collected from the southwest of China. Additionally, the genetic backgrounds of all cultivars were relatively narrow and a limited number of cultivars (15.8%) contained both potato late blight and potato virus Y resistance markers. Also, only two accessions (Yunshu 103 and Lishu 7) contained both late blight and potato virus X resistance markers. In conclusion, this comprehensive evaluation of genetic resources will shed the light on potato disease resistance breeding in the future.
显示更多 [+] 显示较少 [-]Germplasm evaluation and molecular selection of potato (Solanum tuberosum L.) cultivars with disease resistance in China 全文
2017
Li, W.L. | Guo, W. | Xiao, J.P. | Bai, L. | Guo, H.C.
Foreground and background selections are two important aspects that need to be carefully considered by plant breeders during field selection. In this article, we used 7 disease resistance markers, including four late blight and three potato virus disease resistance gene markers, and 12 microsatellite markers to evaluate the disease resistance and genetic diversity of 76 potato cultivars in total which were collected from 15 provinces of China. The foreground selection results showed that a number of materials, clustered separately, contained more than two late blight resistance markers or pyramided late blight and virus disease resistance gene markers together. A lot of them were collected from the southwest of China. Additionally, the genetic backgrounds of all cultivars were relatively narrow and a limited number of cultivars (15.8%) contained both potato late blight and potato virus Y resistance markers. Also, only two accessions (Yunshu 103 and Lishu 7) contained both late blight and potato virus X resistance markers. In conclusion, this comprehensive evaluation of genetic resources will shed the light on potato disease resistance breeding in the future.
显示更多 [+] 显示较少 [-]Germplasm Evaluation and Molecular Selection of Potato (Solanum tuberosum L.) Cultivars with Disease Resistance in China 全文
2017
Li W L, Guo W, Xiao J P et al.
Foreground and background selections are two important aspects that need to be carefully considered by plant breeders during field selection. In this article, we used 7 disease resistance markers, including four late blight and three potato virus disease resistance gene markers, and 12 microsatellite markers to evaluate the disease resistance and genetic diversity of 76 potato cultivars in total which were collected from 15 provinces of China. The foreground selection results showed that a number of materials, clustered separately, contained more than two late blight resistance markers or pyramided late blight and virus disease resistance gene markers together. A lot of them were collected from the southwest of China. Additionally, the genetic backgrounds of all cultivars were relatively narrow and a limited number of cultivars (15.8%) contained both potato late blight and potato virus Y resistance markers. Also, only two accessions (Yunshu 103 and Lishu 7) contained both late blight and potato virus X resistance markers. In conclusion, this comprehensive evaluation of genetic resources will shed the light on potato disease resistance breeding in the future.
显示更多 [+] 显示较少 [-]Germplasm evaluation and molecular selection of potato (Solanum tuberosum L.) cultivars with disease resistance in China 全文
2017
Li, Wan Lin | Guo, Wen | Xiao, Ji Ping | Bai, Lei | Guo, Hua Chun
Foreground and background selections are two important aspects that need to be carefully considered by plant breeders during field selection. In this article, we used 7 disease resistance markers, including four late blight and three potato virus disease resistance gene markers, and 12 microsatellite markers to evaluate the disease resistance and genetic diversity of 76 potato cultivars in total which were collected from 15 provinces of China. The foreground selection results showed that a number of materials, clustered separately, contained more than two late blight resistance markers or pyramided late blight and virus disease resistance gene markers together. A lot of them were collected from the southwest of China. Additionally, the genetic backgrounds of all cultivars were relatively narrow and a limited number of cultivars (15.8%) contained both potato late blight and potato virus Y resistance markers. Also, only two accessions (Yunshu 103 and Lishu 7) contained both late blight and potato virus X resistance markers. In conclusion, this comprehensive evaluation of genetic resources will shed the light on potato disease resistance breeding in the future.
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