The Ac/Ds transposon system from maize as a tool for generating mutant phenotypes in tomato (Lycopersicon esculentum)
1998
Spasieva, S. | Stoyanova, R. | Dukjandzhiev, S. (University of Plovdiv, Plovdiv (Bulgaria)) | Brandwagt, B. | Hille, J. (Institute for Molecular Biological Sciences, BioCentrum Amsterdam, Vrije Universitet, Amsterdam (Netherlands))
A non-targeted transposon tagging experiment was performed in tomato (Lycopersicon esculentum). The Ac/Ds transposon system from maize was used. To generate a transposon tagging population, tomato genotypes ET 570 and SLJ10512 were used. The ET 570 genotype contains a single homozygous T-DNA with Ds at the end of the long arm of chromosome 3. The SLJ10512 genotype carries a stabilized Ac that can activate Ds in trans. This tagging population was tested at the seedling level and two phenotypic mutants were found that co-segregated with a Ds insertion: feebly and yellow jim.Phenotypic mutants, obtained from this transposon tagging experiment, that show linkage to the Ds insertion, might be used to isolate the genes responsible for the mutant phenotypes.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل Institute of Agricultural Economics