The Ac/Ds transposon system from maize as a tool for generating mutant phenotypes in tomato (Lycopersicon esculentum)
1998
Spasieva, S. | Stoyanova, R. | Dukjandzhiev, S. (University of Plovdiv, Plovdiv (Bulgaria)) | Brandwagt, B. | Hille, J. (Institute for Molecular Biological Sciences, BioCentrum Amsterdam, Vrije Universitet, Amsterdam (Netherlands))
A non-targeted transposon tagging experiment was performed in tomato (Lycopersicon esculentum). The Ac/Ds transposon system from maize was used. To generate a transposon tagging population, tomato genotypes ET 570 and SLJ10512 were used. The ET 570 genotype contains a single homozygous T-DNA with Ds at the end of the long arm of chromosome 3. The SLJ10512 genotype carries a stabilized Ac that can activate Ds in trans. This tagging population was tested at the seedling level and two phenotypic mutants were found that co-segregated with a Ds insertion: feebly and yellow jim.Phenotypic mutants, obtained from this transposon tagging experiment, that show linkage to the Ds insertion, might be used to isolate the genes responsible for the mutant phenotypes.
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