Inactivation of SARS coronavirus by means of povidone-iodine, physical conditions, and chemical reagents
2004
Kariwa, H. (Hokkaido Univ., Sapporo (Japan)) | Fujii, N. | Takashima, I.
The efficacy of several povidone-iodine (PVP-I) products, a number of other chemical agents, and various physical conditions were evaluated for their ability to inactivate the severe acute respiratory syndrome coronavirus (SARS-CoV). Treatment of SARS-CoV with PVP-I products for 2 min reduced the virus infectivity from 1.17 x 10**6 TCID sub(50)/ml to below the detectable level. The efficacy of 70% ethanol was equivalent to that of PVP-I products. Fixation of SARS-CoV-infected Vero E6 cells with a fixative including formalin, glutaraldehyde, methanol, and acetone for 5 min or longer eliminated all infectivity. Heating the virus at 56 deg C for 5 min dramatically reduced the infectivity of the virus from 2.6 x 10**7 to 40 TCID sub(50)/ml, whereas heating the virus for 60 min or longer eliminated all infectivity. Irradiation with ultraviolet light at 134MicroW/square cm for 15 min reduced the infectivity from 3.8 x 10**7 to 180 TCID sub(50)/ml; however, prolonged irradiation (60 min) failed to eliminate the remaining virus, leaving 18.8 TCID sub(50)/ml. We believe that these findings will be useful for the implementation of infection contiol measures against SARS, and for the establishment of effective guidelines for the prevention of SARS outbreaks.
اظهر المزيد [+] اقل [-]