Characterization of Endoglucanase (F-Ⅰ-Ⅲ) Purified from Trichoderma sp. C-4
2005
Sul, O.J. (University of Ulsan, Ulsan, Republic of Korea) | Chung, D.K. (Kyung Hee University, Suwon, Republic of Korea) | Han, I.S. (University of Ulsan, Ulsan, Republic of Korea) | Jeong, C.S. (University of Ulsan, Ulsan, Republic of Korea), E-mail: csjeong@mail.ulsan.ac.kr
One of the endoglucanases, F-Ⅰ-Ⅲ, was purified from the culture filtrate of T. sp. C-4 through procedures including chromatography on Sephacryl S-200, DEAE-Sepharose A-50, and Chromatofocusing on Mono-P (FPLC). The molecular weight of the enzyme was determined to be about 56,000 Da by SDS-PAGE, and pI of 4.9 by analytical isoelectric focusing. F-Ⅰ-Ⅲ showed the highest enzyme activity at 55℃, and the pH optimum of the enzyme was 5.0. There was no loss of activity when the enzyme was incubated at 50℃ for 24 hours.
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