Characterization of Endoglucanase (F-Ⅰ-Ⅲ) Purified from Trichoderma sp. C-4

Sul, O.J.; Chung, D.K.; Han, I.S.; Jeong, C.S. , E-mail: csjeong@mail.ulsan.ac.kr

Characterization of Endoglucanase (F-Ⅰ-Ⅲ) Purified from Trichoderma sp. C-4

2005

Sul, O.J. (University of Ulsan, Ulsan, Republic of Korea) | Chung, D.K. (Kyung Hee University, Suwon, Republic of Korea) | Han, I.S. (University of Ulsan, Ulsan, Republic of Korea) | Jeong, C.S. (University of Ulsan, Ulsan, Republic of Korea), E-mail: csjeong@mail.ulsan.ac.kr

One of the endoglucanases, F-Ⅰ-Ⅲ, was purified from the culture filtrate of T. sp. C-4 through procedures including chromatography on Sephacryl S-200, DEAE-Sepharose A-50, and Chromatofocusing on Mono-P (FPLC). The molecular weight of the enzyme was determined to be about 56,000 Da by SDS-PAGE, and pI of 4.9 by analytical isoelectric focusing. F-Ⅰ-Ⅲ showed the highest enzyme activity at 55℃, and the pH optimum of the enzyme was 5.0. There was no loss of activity when the enzyme was incubated at 50℃ for 24 hours.

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Ключевые слова АГРОВОК

trichoderma

Библиографическая информация

Опубликовано в

The Korean Journal of Microbiology

Том 41 Выпуск 1 ISSN 0440-2413

Нумерация страниц

pp. 81-86

Другие темы

C-4; Characterization; 4-β-glucanase (f-ⅰ-ⅲ); Endo-1; Protein sequencing

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Примечание

Summary(En)

2 tables

6ill., 32 ref.

Переведенный заголовок

새로운 섬유소분해 균주 Trichoderma sp. C-4에서 분리한 Endoglucanase (F-Ⅰ-Ⅲ)에 대한 연구

Тип

AGRIS — международная информационная система по сельскохозяйственным наукам и технологиям

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Characterization of Endoglucanase (F-Ⅰ-Ⅲ) Purified from Trichoderma sp. C-4

2005

Ключевые слова АГРОВОК

Библиографическая информация