Rapid isolation of DNA from mucus of Asian arowana (Scleropages formosus, Osteoglossidae)

Nantarika Chansue. Faculty of Veterinary Sciences. Faculty of Veternary Sciences. Veterinary Medical Aquatic Animal Research Center; Krittima Anekthnakul. Faculty of Veterinary Sciences. Faculty of Veternary Sciences. Veterinary Medical Aquatic Animal Research Center; Sujettana Sothipan. Scientific Crime Direction Div.; Krissada Ribruamsup

Rapid isolation of DNA from mucus of Asian arowana (Scleropages formosus, Osteoglossidae)

2005

Nantarika Chansue(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Sciences. Faculty of Veternary Sciences. Veterinary Medical Aquatic Animal Research Center) | Krittima Anekthnakul(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Sciences. Faculty of Veternary Sciences. Veterinary Medical Aquatic Animal Research Center) | Sujettana Sothipan(Royal Thai Police, Bangkok (Thailand). Scientific Crime Direction Div.) | Krissada Ribruamsup(Central Institute of Forensic Science, Bangkok (Thailand))

DNA of Dragon fish or Asian arowana was extracted by Phenol-chloroform method using DNA from mucus of Dragon fish collected by using cotton swab. The concentration and purity of the DNA was determined by a spectrophotometer, at optical densities of 260 nm and 280 nm and agarose gel electrophoresis. Studying of DNA quality for example DNA molecule compared with standard DNA , RNA contamination , DNA degradation , other contamination and DNA quantity using agarose gel electrophoresis. From the result, DNA quality was good criterion and a little RNA contamination. Statistic test result performed on the concentration of mucus DNA and gill DNA did not differ significantly between the groups at 95 percent confident a level.

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