Analysis of the optimized factors on the efficiency of Agrobacterium mediated gene transformation | 优化农杆菌介导基因转化效率因子分析
2010
Liu Caiping, Chinese Academy of Forestry，Guangzhou(China), Breeding Center of Tropical Forestry Institute | Zeng Bingshan, Chinese Academy of Forestry，Guangzhou(China), Breeding Center of Tropical Forestry Institute | Qiu Zhenfei, Chinese Academy of Forestry，Guangzhou(China), Breeding Center of Tropical Forestry Institute
صينى. 共培养法是农杆菌介导转基因比较常用的方法之一。建立一个高效的遗传转化体系，除了确定抗生素种类，菌种和菌液浓度，预培养和共培养时间等基本因子外，优化影响转化的因子非常重要。主要介绍和分析共培养法中可能优化的影响基因转化因子，为提高农杆菌介导转化率提供了方法和依据。
اظهر المزيد [+] اقل [-]إنجليزي. Co-culture is one of the Agrobacterium mediated gene transformation methods. In addition to the choice of antibiotics，Agrobacterium strain and optical density，time of the pre-culture and co-culture，optimization of factors affecting transformation are also very important in developing an efficient genetic transformation protocol. The optimized factors were introduced in co-culture method in order to provide methods and basis for enhancing the efficiency of Agrobacterium mediated transformation.
اظهر المزيد [+] اقل [-]