Impact of copper to the chelation effect of bovine serum albumin and spermatozoa motility parameter in vitro

The target of this in vitro study was to analyse the influence of copper on the spermatozoa motility in the presence of bovine serum albumin (BSA) as culture medium and to provide additional information on the interaction between serum albumin and copper (II) chloride (CuCl2). The spermatozoa motility parameters was determined after exposure of CuCl2 (3.9; 7.8; 15.6; 31.2; 62.5; 125; 250; 500; 1000 micromol/L) using the Sperm VisionTM CASA (Computer Assisted Semen Analyzer) system during different time periods (Time 0 h, 1 h, 2 h and 24). The culture medium containing 20% BSA, triladyl and 5% glucose increased the overall percentage of spermatozoa motility after 1 h of cultivation. The percentage of motility spermatozoa significantly (P is less than 0.001) decreased after 2 h of cultivation at the concentrations is greater than or equal to 250 micromol/L of CuCl2 in comparison with the control group (without CuCl2 administration). The experimental administration at the doses is less than or equal to 31.2 micromol/L of CuCl2 stimulated the overall of motile spermatozoa (Time 24 h). Identical spermatozoa motility was detected also for the percentage of progressive motile spermatozoa during all time periods. Parameter of distance average path (DAP) showed increase in all CuCl2 addition groups in comparison with the control group at Time 1 h. Concentration 125 micromol/L of CuCl2 in various time periods of cultivation act stimulating on spermatozoa motility, but later (Time 24 h) inhibitory. Evaluation of velocity average path, showed similar results as for DAP. Measurement of the amplitude of lateral displacement (ALH) at Time 0 h as well as at Time 1 h was higher in all the experimental groups compared to the control group, but the differences were not significant (P is greater than 0.05). The experimental administration at the doses is less than or equal to 62.5 micromol/L of CuCl2 stimulated ALH during 24 h of cultivation. The results suggest that adding energy and protein substrate to the culture medium increases the spermatozoa motility parameters also the presence of high doses (is greater than or equal to 125 micromol/L) of copper ions during short-term periods (Time 0 h, 1 h). Concurrently BSA maintained motility of spermatozoa (is less than or equal to 31.20 micromol/L of CuCl2) during the long-term (Time 24 h) of cultivation, which confirms the protective effect of albumin binding to the copper ions.