Alteration of beta-tubulin in Nicotiana plumbaginifolia confers resistance to amiprophos-methyl

Blume, Ya.B.; Strashnyuk, N.M.; Smertenko, A.P.; Solodushko, V.G.; Sidorov, V.A.; Gleba, Yu.Yu

Alteration of beta-tubulin in Nicotiana plumbaginifolia confers resistance to amiprophos-methyl

1998

A Nicotiana plumbaginifolia plant (apm5r) resistant to amiprophos-methyl (APM), a phosphoroamide herbicide, was isolated from protoplasts prepared from leaves of haploid plants. Genetic analysis revealed that the resistance is coded for by a dominant nuclear mutation and is associated with the increased stability of cortical microtubules. Two-dimensional polyacrylamide-gel electrophoresis, combined with immunoblotting using anti-tubulin monoclonal antibodies, showed that part of the beta-tubulin in the resistant plant possessed lower isoelectric points than the beta-tubulin of susceptible wild-type plants. These results provide evidence that the resistance to APM is associated with a mutation in a beta-tubulin gene. The APM-resistant line showed cross-resistance to trifluralin, a dinitroaniline herbicide, suggesting a common mechanism of resistance between these two classes of herbicides.

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