Alteration of beta-tubulin in Nicotiana plumbaginifolia confers resistance to amiprophos-methyl
1998
Blume, Ya.B. | Strashnyuk, N.M. | Smertenko, A.P. | Solodushko, V.G. | Sidorov, V.A. | Gleba, Yu.Yu
A Nicotiana plumbaginifolia plant (apm5r) resistant to amiprophos-methyl (APM), a phosphoroamide herbicide, was isolated from protoplasts prepared from leaves of haploid plants. Genetic analysis revealed that the resistance is coded for by a dominant nuclear mutation and is associated with the increased stability of cortical microtubules. Two-dimensional polyacrylamide-gel electrophoresis, combined with immunoblotting using anti-tubulin monoclonal antibodies, showed that part of the beta-tubulin in the resistant plant possessed lower isoelectric points than the beta-tubulin of susceptible wild-type plants. These results provide evidence that the resistance to APM is associated with a mutation in a beta-tubulin gene. The APM-resistant line showed cross-resistance to trifluralin, a dinitroaniline herbicide, suggesting a common mechanism of resistance between these two classes of herbicides.
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