Enhanced electrochemiluminescence of peroxydisulfate by electrodeposited Au nanoparticles and its biosensing application via integrating biocatalytic precipitation using self-assembly bi-enzymes
2013
Gao, Bu-Hong | Ding, Shou-Nian | Kargbo, Osman | Wang, Yi-Hong | Sun, Yue-Ming | Cosnier, Serge
The enhanced electrochemiluminescence (ECL) of peroxydisulfate was observed on Au nanoparticles (Au NPs) electrodeposited on ITO electrode. Glucose oxidase (GOD) and horseradish peroxidase (HRP) were self-assembled upon Au NPs. Thus, a “signal-off” ECL biosensor to detect glucose by integrating biocatalytic precipitation (BCP) has been achieved. The morphologies of Au NPs and GOD-HRP/Au NPs modified ITO were characterized by scanning electron microscopy (SEM). The electrochemical behaviors of the Au NPs/ITO, GOD-HRP/Au NPs/ITO and BCP/GOD-HRP/Au NPs/ITO were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The resulted biosensor combined the ECL amplification by Au NPs with the self-assembled bi-enzymes integrated BCP system presents a wide linear range from 8.0×10⁻⁷ to 2.0×10⁻²M, and with a high sensitivity of 1.0×10⁻⁷M and good stability for glucose detection.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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