Enhanced electrochemiluminescence of peroxydisulfate by electrodeposited Au nanoparticles and its biosensing application via integrating biocatalytic precipitation using self-assembly bi-enzymes

Gao, Bu-Hong; Ding, Shou-Nian; Kargbo, Osman; Wang, Yi-Hong; Sun, Yue-Ming; Cosnier, Serge

Enhanced electrochemiluminescence of peroxydisulfate by electrodeposited Au nanoparticles and its biosensing application via integrating biocatalytic precipitation using self-assembly bi-enzymes

2013

The enhanced electrochemiluminescence (ECL) of peroxydisulfate was observed on Au nanoparticles (Au NPs) electrodeposited on ITO electrode. Glucose oxidase (GOD) and horseradish peroxidase (HRP) were self-assembled upon Au NPs. Thus, a “signal-off” ECL biosensor to detect glucose by integrating biocatalytic precipitation (BCP) has been achieved. The morphologies of Au NPs and GOD-HRP/Au NPs modified ITO were characterized by scanning electron microscopy (SEM). The electrochemical behaviors of the Au NPs/ITO, GOD-HRP/Au NPs/ITO and BCP/GOD-HRP/Au NPs/ITO were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The resulted biosensor combined the ECL amplification by Au NPs with the self-assembled bi-enzymes integrated BCP system presents a wide linear range from 8.0×10⁻⁷ to 2.0×10⁻²M, and with a high sensitivity of 1.0×10⁻⁷M and good stability for glucose detection.

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