The comparison of different ELISA procedures in detecting anti-Trichinella IgG in human infections

Moskwa, B.; Bien, J.; Cabaj, W.; Kořinková, K.; Koudela, B.; Stefaniak, J.

The comparison of different ELISA procedures in detecting anti-Trichinella IgG in human infections

2009

The aim of this study was to compare the performance of four different ELISA procedures in diagnosing Trichinella spp. infections in human outbreaks. The main differences between the various procedures were: antigen concentration, dilutions of human serum sample, the type of conjugate used, and time of conjugate incubation. Serum samples were collected from 23 individuals involved in an outbreak. Results obtained in the four tested ELISA procedures differed in the rate of positive results obtained for the examined outbreak. Western blot confirmed the ELISA-positive results. Reactions to the specific 41-45kDa and 55kDa bands were obtained with all positive sera.

اظهر المزيد [+]

الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)

accuracy adolescent aged animals antibodies antigens blood diagnostic techniques disease outbreaks disease outbreaks epidemiology health helminth humans humans immunology methodology methods molecular weight parasitology poland trichinella trichinosis western blotting

المعلومات البيبليوغرافية

نُشرت في

Veterinary parasitology

المجلد 159 الإصدار 3 ترقيم الصفحات 312 - 315 الرقم التسلسلي المعياري الدولي (ردمد) 0304-4017

الناشر

Elsevier

مواضيع أخرى

Animal parasitic nematodes; Child; Enzyme-linked immunosorbent assay; And safety; Human medicine; Immunoglobulin g; Disease detection; Diet and diet-related diseases; Trichinellosis; Young adult; Enzyme-linked immunosorbent assay; Adult; Immunoglobulin g; Middle aged; Disease diagnosis; Female; Male; Serologic tests

اللغة

إنجليزي

ملاحظة

In the special issue: selected papers presented at the 12th international conference on trichinellosis - plitvice lakes national park, croatia / edited by alvin a. gajadhar, h. ray gamble, delores hill, albert marinculic and dante zarelnga.

Includes references

2019-12-06

النوع

Journal Article; Text

في أجريس منذ: 2024-02-28

نوع الملف: MODS

مزود البيانات

تم تزويد هذا السجل من قبل National Agricultural Library

اكتشف مجموعة مزود البيانات هذا في أجريس

الروابط

DOI http://dx.doi.org/10.1016/j.vetpar.2008.10.069

تصفح الباحث العلمي من جوجل