The comparison of different ELISA procedures in detecting anti-Trichinella IgG in human infections

Moskwa, B.; Bien, J.; Cabaj, W.; Kořinková, K.; Koudela, B.; Stefaniak, J.

The comparison of different ELISA procedures in detecting anti-Trichinella IgG in human infections

2009

The aim of this study was to compare the performance of four different ELISA procedures in diagnosing Trichinella spp. infections in human outbreaks. The main differences between the various procedures were: antigen concentration, dilutions of human serum sample, the type of conjugate used, and time of conjugate incubation. Serum samples were collected from 23 individuals involved in an outbreak. Results obtained in the four tested ELISA procedures differed in the rate of positive results obtained for the examined outbreak. Western blot confirmed the ELISA-positive results. Reactions to the specific 41-45kDa and 55kDa bands were obtained with all positive sera.

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Palabras clave de AGROVOC

accuracy adolescent aged animals antibodies antigens blood diagnostic techniques disease outbreaks disease outbreaks epidemiology health helminth humans humans immunology methodology methods molecular weight parasitology poland trichinella trichinosis western blotting

Información bibliográfica

Publicado en

Veterinary parasitology

Volumen 159 Edición 3 Paginación 312 - 315 ISSN 0304-4017

Editorial

Elsevier

Otras materias

Animal parasitic nematodes; Child; Enzyme-linked immunosorbent assay; And safety; Human medicine; Immunoglobulin g; Disease detection; Diet and diet-related diseases; Trichinellosis; Young adult; Enzyme-linked immunosorbent assay; Adult; Immunoglobulin g; Middle aged; Disease diagnosis; Female; Male; Serologic tests

Idioma

Inglés

Nota

In the special issue: selected papers presented at the 12th international conference on trichinellosis - plitvice lakes national park, croatia / edited by alvin a. gajadhar, h. ray gamble, delores hill, albert marinculic and dante zarelnga.

Includes references

2019-12-06

Tipo

Journal Article; Text

En AGRIS desde: 2024-02-28

Formato: MODS

Proveedor de Datos

Este registro bibliográfico ha sido proporcionado por National Agricultural Library

Descubra la colección de este proveedor de datos en AGRIS

Enlaces

DOI http://dx.doi.org/10.1016/j.vetpar.2008.10.069

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