Characterization of corrinoid compounds from a Japanese black tea (Batabata-cha) fermented by bacteria

Kittaka-Katsura, H.; Ebara, S.; Watanabe, F.; Nakano, Y.

Characterization of corrinoid compounds from a Japanese black tea (Batabata-cha) fermented by bacteria

2004

Kittaka-Katsura, H. | Ebara, S. | Watanabe, F. | Nakano, Y.

A Japanese fermented black tea (Batabata-cha) contained a considerable amount of vitamin B12 (456 ± 39 ng per 100 g dry tea leaves and 2.0 ± 0.3 ng per 100 mL of tea drink). A corrinoid compound was partially purified and characterized from the tea leaves. The patterns of the purified compound by the silica gel 60 thin-layer chromatography and C18 reversed phased high-performance liquid chromatography were identical to those of authentic vitamin B12. When 20 week old vitamin B12 deficient rats, which excreted substantial amounts (about 250 mg/day) of methylmalonic acid in urine as an index of vitamin B12 deficiency, were fed the tea drink (50 mL/day, 1 ng of vitamin B12) for 6 weeks, urinary methylmalonic acid excretion (169 ± 29 mg/day) of the tea drink-supplemented 26 week old rats decreased significantly relative to that (250 ± 32 mg/day) of the deficient rats. The results indicate that the vitamin B12 found in the fermented black tea is bioavailable in mammals.

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