Optimization of cryoprotectants for improved survival rates of animal probiotics Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae
2024
Jang, I.H. | Lee, A.R. | Song, I.G. | Kim, S.K.
Three probiotics, Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae isolated from chicken cecum were investigated by adding various cryoprotectants to improve viability after both frozen-storage and freeze-drying. During frozen-storage at -20°C, glycerol provided a higher survival rate (15.8–58.3%) than other cryoprotectants for the probiotics. The effect of the cryoprotectants decreased with an increased storage time of 30 days. Bacillus subtilis preserved in a 1:1 mixture of glycerol and mineral oil showed a higher survival rate of 38.1% compared to glycerol only with a survival rate of 30.95%, demonstrating the synergistic effect of the cryoprotectants. The relationship between cryoprotectants and the survival rate at -80°C freeze-drying was also studied. An effective cryoprotectant for L. plantarum was found to be 20% soy peptone/ 1% lactose with a survival rate of 80%. In B. subtilis, 10% soy peptone/5% lactose presented effective synergistic cryoprotectants with a survival rate of 92.9%, whereas in S. cerevisiase, 10% soy peptone 1% mannose showed a survival rate of 52.2%. In conclusion, we demonstrated that an optimization of the ideal proportion of the cryoprotectants glycerol and soy peptone to sugar increased cell survivability for storage according to the respective probiotics.
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