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MDA and GSH-Px activity in transition dairy cows under seasonal variations and their relationship with reproductive performance النص الكامل
2017
Colakoglu, Hatice Esra | Yazlik, Murat Onur | Kaya, Ufuk | Colakoglu, Ekrem Cagatay | Kurt, Serdal | Oz, Burcu | Bayramoglu, Rustem | Vural, Mehmet Rifat | Kuplulu, Sukru
MDA and GSH-Px activity in transition dairy cows under seasonal variations and their relationship with reproductive performance النص الكامل
2017
Colakoglu, Hatice Esra | Yazlik, Murat Onur | Kaya, Ufuk | Colakoglu, Ekrem Cagatay | Kurt, Serdal | Oz, Burcu | Bayramoglu, Rustem | Vural, Mehmet Rifat | Kuplulu, Sukru
Introduction: The purpose of the current study was to evaluate the blood glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) levels under seasonal variations in dairy cows during transition period, and to assess the relationship between chosen reproductive parameters, GSH-Px, and MDA. Material and Methods: Holstein cows calving in January were assigned into winter group (n = 42), while cows calving in August were assigned into summer group (n = 42). Blood samples were collected from the jugular vein 21, 14, and 7 days before calving, at calving (0 day), and 7, 14, and 21 days after calving. Reproductive parameters obtained from farm records were evaluated. Results: In both groups of cows, GSH-Px activity decreased from 21 days before calving to day 0, and it gradually continued to increase until 21 days after calving. GSH-Px activity was higher in winter group compared to summer group during the transition period (P < 0.05). MDA levels in both groups increased over time starting from 21 days before calving to 0 day, but it gradually decreased thereafter. MDA levels were higher in summer group compared to winter group during the transition periods (P < 0.05). Summer group of cows showed higher intervals of calving-to-oestrus, calving-to-conception, and higher insemination index (P < 0.01). Negative correlation was recorded between GSH-Px and MDA during all examination days (P < 0.01). MDA levels correlated with calving to conception interval on day 21 before calving and day 0 (P < 0.01) and insemination index on day 0 and 21 days after calving (P < 0.01). GSH-Px activity was negatively correlated with calving to conception interval on day 21 before calving, day 0, and 21 days (P < 0.01) after calving. Negative correlation on day 21 before calving and day 0 was also determined between GSH-Px and insemination index (P < 0.01). Conclusion: This study showed that blood oxidant and antioxidant levels have affected the fertility parameters in cows under seasonal variations.
اظهر المزيد [+] اقل [-]MDA and GSH-Px activity in transition dairy cows under seasonal variations and their relationship with reproductive performance النص الكامل
2017
Colakoglu Hatice Esra | Yazlik Murat Onur | Kaya Ufuk | Colakoglu Ekrem Cagatay | Kurt Serdal | Oz Burcu | Bayramoglu Rustem | Vural Mehmet Rifat | Kuplulu Sukru
Introduction: The purpose of the current study was to evaluate the blood glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) levels under seasonal variations in dairy cows during transition period, and to assess the relationship between chosen reproductive parameters, GSH-Px, and MDA.
اظهر المزيد [+] اقل [-]Were Polish wild boars exposed to Schmallenberg virus? النص الكامل
2017
Kęsik-Maliszewska, Julia | Jabłoński, Artur | Larska, Magdalena
Were Polish wild boars exposed to Schmallenberg virus? النص الكامل
2017
Kęsik-Maliszewska, Julia | Jabłoński, Artur | Larska, Magdalena
Introduction: A novel to Europe Schmallenberg virus (SBV) causes clinical disease manifested by reproduction disorders in farm ruminants. In free-living ruminants, SBV antibodies as well as the virus were detected. Recent studies also revealed SBV antibodies in wild boars. The study investigates SBV antibodies occurring in wild boars in Poland at the peak of recent virus epidemics in the country.Material and Methods: Samples collected from 203 wild boars culled during the 2012/2013 and 2013/2014 hunting season were serologically tested using multi-species cELISA. Attempted neutralisation tests failed due to poor serum quality. RT-PCR was implemented in seropositive and doubtful animals.Results: Two samples collected from wild boar in the winter of 2013 gave a positive result in ELISA, while another two from the 2012/2013 hunting season were doubtful. No SBV RNA was detected in spleen and liver tissues.Conclusion: Low SBV seroprevalence in wild boars, despite high incidence of SBV infections occurring simultaneously in wild ruminants, suggests that boars are unlikely to be a significant reservoir of the virus in the sylvatic environment in Poland.
اظهر المزيد [+] اقل [-]Were Polish wild boars exposed to Schmallenberg virus? النص الكامل
2017
Kęsik-Maliszewska Julia | Jabłoński Artur | Larska Magdalena
Introduction: A novel to Europe Schmallenberg virus (SBV) causes clinical disease manifested by reproduction disorders in farm ruminants. In free-living ruminants, SBV antibodies as well as the virus were detected. Recent studies also revealed SBV antibodies in wild boars. The study investigates SBV antibodies occurring in wild boars in Poland at the peak of recent virus epidemics in the country.
اظهر المزيد [+] اقل [-]Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region النص الكامل
2022
Ijiri, Moe | Odo, Kenji | Sato, Motohiko | Kawaguchi, Maiko | Fujimoto, Yoshikazu | Miura, Naoki | Matsuo, Tomohide | Hou, De-Xing | Yamato, Osamu | Tanabe, Takashi | Kawaguchi, Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region النص الكامل
2022
Ijiri, Moe | Odo, Kenji | Sato, Motohiko | Kawaguchi, Maiko | Fujimoto, Yoshikazu | Miura, Naoki | Matsuo, Tomohide | Hou, De-Xing | Yamato, Osamu | Tanabe, Takashi | Kawaguchi, Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region were investigated by comparing the biomarker changes in the summer (a period of chronic heat stress) and winter (a thermoneutral period) seasons. Pigs were allocated to summer- and winter-finishing cohorts, 12 each. The evaluations included assessment of carcass traits and internal organs’ normality carried out at the time of slaughter, and measurement of biomarkers in whole blood: derivatives of reactive oxygen metabolites (d-ROMs) and biological antioxidant potential as markers of oxidative stress, and serum amyloid A and albumin/globulin (A/G) ratio as markers of acute and chronic inflammation, respectively. The summer-finished pigs reared under subtropical field conditions showed lower carcass quality than the winter-finished pigs, indicating a potential adverse effect of summer temperatures on the swine industry. Marginal changes were observed in d-ROMs and the A/G ratio between the summer- and winter-finishing cohorts. The results demonstrate that d-ROMs and the A/G ratio could be used as sensitive markers for heat stress under field conditions.
اظهر المزيد [+] اقل [-]Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region النص الكامل
2022
Ijiri Moe | Odo Kenji | Sato Motohiko | Kawaguchi Maiko | Fujimoto Yoshikazu | Miura Naoki | Matsuo Tomohide | Hou De-Xing | Yamato Osamu | Tanabe Takashi | Kawaguchi Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region were investigated by comparing the biomarker changes in the summer (a period of chronic heat stress) and winter (a thermoneutral period) seasons.
اظهر المزيد [+] اقل [-]Parasitological monitoring of European bison (Bison bonasus) from three forests of north-eastern Poland between 2014 and 2016 النص الكامل
2020
Krzysiak, Michał K. | Demiaszkiewicz, Aleksander W. | Larska, Magdalena | Tomana, Jarosław | Anusz, Krzysztof
Parasitological monitoring of European bison (Bison bonasus) from three forests of north-eastern Poland between 2014 and 2016 النص الكامل
2020
Krzysiak, Michał K. | Demiaszkiewicz, Aleksander W. | Larska, Magdalena | Tomana, Jarosław | Anusz, Krzysztof
Common parasites of the European bison include gastro-intestinal and pulmonary nematodes, liver flukes (Fasciola hepatica), tapeworms, and protozoa of the genus Coccidia. This study compared the extensiveness and intensities of European bison parasitic invasions in three north-eastern Polish forests in different seasons and queried the role of parasitological monitoring in sanitary and hygienic control of feeding places. Faecal samples were collected in the Białowieża, Knyszyńska, and Borecka Forests between 2014 and 2016, as were some from an area neighbouring the Białowieża Forest outside the Natura 2000 protected area. Parasites were detected in individual samples with the flotation, decanting and Baermann methods. The eggs of Trichostrongylidae, Aonchotheca sp., Nematodirus sp., Strongyloides spp., Trichuris sp., Moniezia spp., and Fasciola hepatica; the larvae of Dictyocaulus viviparus; and the oocytes of Eimeria spp. were identified. Significant variation in invasion intensity and diversity was seen by origin and season. The relationships were assessed first by univariable tests and next multivariately, when origin and season emerged as the major risk factors for exposure to most of the parasites. The differences in the level of parasitic infection between the forests did not have implications for its sufficiency to cause clinical symptoms. However, the associations and risk factors found enable the necessary preventive measures to be taken to protect the E. bison from exposure or decrease the risks. Additionally, parasitological monitoring is appropriate as the method of sanitary and hygienic control of European bison winter feeding places. Threats to public health through adventitious invasions by zoonotic factors such as F. hepatica have been identified.
اظهر المزيد [+] اقل [-]Parasitological monitoring of European bison (Bison bonasus) from three forests of north-eastern Poland between 2014 and 2016 النص الكامل
2020
Krzysiak Michał K. | Demiaszkiewicz Aleksander W. | Larska Magdalena | Tomana Jarosław | Anusz Krzysztof
Common parasites of the European bison include gastro-intestinal and pulmonary nematodes, liver flukes (Fasciola hepatica), tapeworms, and protozoa of the genus Coccidia. This study compared the extensiveness and intensities of European bison parasitic invasions in three north-eastern Polish forests in different seasons and queried the role of parasitological monitoring in sanitary and hygienic control of feeding places.
اظهر المزيد [+] اقل [-]Microbiological quality of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish from Eastern Poland النص الكامل
2018
Pyz-Łukasik, Renata | Paszkiewicz, Waldemar
Microbiological quality of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish from Eastern Poland النص الكامل
2018
Pyz-Łukasik, Renata | Paszkiewicz, Waldemar
The purpose of this study was to determine the microbiological quality of food fish and its safety for consumers. The study included 24 fish representing grass carp, bighead carp, Siberian sturgeon, and wels catfish. Specimens were collected in winter. Aerobic bacteria, psychrophilic, Enterobacteriaceae, Staphylococcus spp., and E. coli counts were made, and the presence of Salmonella spp., L. monocytogenes, S. aureus, and other coagulase-positive staphylococci was investigated. The microbiological analysis showed a similar level of aerobic, psychrophilic, and Staphylococcus spp. contamination of the four fish species. The Enterobacteriaceae count was higher in the muscles of grass carp and bighead carp than S. sturgeon and wels catfish. No pathogenic bacteria such as Salmonella spp., E. coli, L. monocytogenes, Staphylococcus aureus, or other coagulase positive staphylococci were found in samples of the examined fish species. The fresh fish examined in this study were of good microbiological quality and there was no health risk for consumers.
اظهر المزيد [+] اقل [-]Microbiological quality of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish from Eastern Poland النص الكامل
2018
Pyz-Łukasik Renata | Paszkiewicz Waldemar
The purpose of this study was to determine the microbiological quality of food fish and its safety for consumers.
اظهر المزيد [+] اقل [-]WANDERING THYROID FOLLICLES WITHIN THE RENAL TISSUE OF CARP FISH "Cyprinus Carpio". النص الكامل
2017
Amer M. Hussin
Twenty healthy adult Carp fish were collected from Dijlah river in Iraq in Summer and winter times of the year (Ten for each ). The fish were sacrificed and fixed in 10% neutral buffered formalin solution. Routine histological preparations were carried out. Hematoxylin - Eosin stain was used. The finding of the current study showed that clear connective tissue capsule was not observed. Instead, wide loose connective tissue was surrounded the follicles. The thyroid follicles were distributed individually or in groups in the kidney and sub pharyngeal region( around the ventral aorta). The thyroid tissue was represented by thyroid follicles of different sizes and shapes. In Carp fish, the building up and organization of the follicular cells of thyroid follicles were simple and poorly organized. There was a close relationship between the morphological state of thyroid follicles and the season( temperature). The results of the present study also demonstrated that the spread of thyroid follicles in summer was more than in winter where the follicles appeared as aggregated at the periphery of renal tissue and also the results of this study showed that large and small follicles were combined. The study also registered that, during winter, the large follicles were mostly formed by the aggregation of smaller ones and that according to the physiological status of the thyroid tissue. The study concluded firstly that these follicles had the ability to wandered within the renal tissue. Moreover, the study concluded that the thyroid follicle was dynamic structure, and their sizes depending on the amount of colloid present within .
اظهر المزيد [+] اقل [-]Relationship between vaginal mucus conductivity and time of ovulation in weaned sows النص الكامل
2015
Hidalgo, David M. | Cassar, Glen | Manjarin, Rodrigo | Dominguez, Juan C. | Friendship, Robert M. | Kirkwood, Roy N.
Relationship between vaginal mucus conductivity and time of ovulation in weaned sows النص الكامل
2015
Hidalgo, David M. | Cassar, Glen | Manjarin, Rodrigo | Dominguez, Juan C. | Friendship, Robert M. | Kirkwood, Roy N.
This study investigated whether changes in the vaginal electrical resistance (VER) of vaginal mucus of weaned sows during the first 7 d post-weaning are associated with time of ovulation. Time of ovulation was determined by ovarian ultrasound carried out from 91 to 146 h after weaning and at different seasons. Vaginal electrical resistance was measured at 20, 44, 68, 91, 96, 102, 115, 120, 126, 140, 146, and 164 h post-weaning and was found to decrease between 120 h and 31 h before ovulation and then increase until 40 to 50 h after ovulation. Duration and timing of the nadir was affected by the season (P < 0.01). Estrus was observed from day 4 after the lowest VER values. Ovulation occurred between late day 5 and late day 6, while VER values were still increasing. Ovulation was earlier in lower parity sows (P < 0.001). Compared to 0 h (ovulation time), VER was significantly lower from 50 to 5 h before ovulation in autumn and from 40 to 21 h in winter, but such differences were not seen in spring. Lowest VER value was not correlated with time of ovulation. It was concluded that VER increases before ovulation and, although this increase is influenced by the season, it cannot be used to accurately predict ovulation in weaned sows.
اظهر المزيد [+] اقل [-]Relationship between vaginal mucus conductivity and time of ovulation in weaned sows النص الكامل
2015
Hidalgo, D. | Cassar, G. | Manjarin, R. | Dominguez, J. | Friendship, R. | Kirkwood, R.
This study investigated whether changes in the vaginal electrical resistance (VER) of vaginal mucus of weaned sows during the first 7 d post-weaning are associated with time of ovulation. Time of ovulation was determined by ovarian ultrasound carried out from 91 to 146 h after weaning and at different seasons. Vaginal electrical resistance was measured at 20, 44, 68, 91, 96, 102, 115, 120, 126, 140, 146, and 164 h post-weaning and was found to decrease between 120 h and 31 h before ovulation and then increase until 40 to 50 h after ovulation. Duration and timing of the nadir was affected by the season (P < 0.01). Estrus was observed from day 4 after the lowest VER values. Ovulation occurred between late day 5 and late day 6, while VER values were still increasing. Ovulation was earlier in lower parity sows (P < 0.001). Compared to 0 h (ovulation time), VER was significantly lower from 50 to 5 h before ovulation in autumn and from 40 to 21 h in winter, but such differences were not seen in spring. Lowest VER value was not correlated with time of ovulation. It was concluded that VER increases before ovulation and, although this increase is influenced by the season, it cannot be used to accurately predict ovulation in weaned sows. | David M. Hidalgo, Glen Cassar, Rodrigo Manjarin, Juan C. Dominguez, Robert M. Friendship, Roy N. Kirkwood
اظهر المزيد [+] اقل [-]Diversity of Salmonella serovars in feedyard and nonfeedyard playas of the Southern High Plains in the summer and winter النص الكامل
2004
Purdy, Charles W. | Straus, David C. | Clark, R Nolan
Objective-To compare Salmonella isolates cultured from feedyard and nonfeedyard (control) playas (ie, temporary shallow lakes) of the Southern High Plains. Sample Population-Water and muck (sediment) samples were obtained from 7 feedyard playas and 3 nonfeedyard playas in the winter and summer. Procedure-Each water and muck sample was enriched with sulfur-brilliant-green broth and incubated in a shaker at 37°C for 24 hours. A sample (100 mL) of the incubated bacterial-enriched broth was then mixed with 100 mL of fresh sulfur-brilliant-green enrichment broth and incubated in a shaker at 37°C for 24 hours. After the second incubation, a swab sample was streaked on differential media. Suspect Salmonella isolates were further identified by use of biochemical tests, and Salmonella isolates were confirmed and serovar determinations made. Results-Salmonella isolates were not recovered from the 3 control playas. Seven Salmonella enterica serovars were isolated from 5 of 7 feedyard playas in the summer, and 13 S enterica serovars were isolated from 7 of 7 feedyard playas in the winter. In the summer, 296 isolates were cultured, and 47 were Salmonella organisms. In the winter, 288 isolates were cultured, and 171 were Salmonella organisms. Conclusions and Clinical Relevance-Results indicated that feedyard playas are frequently contaminated with many Salmonella serovars. These pathogens should be considered whenever feedyard managers contemplate the use of water from these playas. Water from feedyard playas should not be used to cool cattle in the summer or for dust abatement.
اظهر المزيد [+] اقل [-]Seroprevalence of Mycoplasma suis infection in pigs in eastern China as estimated by a blocking enzyme-linked immunosorbent assay النص الكامل
2017
Zhongyang, Liang | Jiansong, Zhang | Yijuan, Shen | Yuting, Xia | Yufeng, Li | Jiarong, Xu
Porcine infectious anemia caused by Mycoplasma suis is a global disease and results in serious economic losses. To determine the prevalence of M. suis infection in eastern China, a cross-sectional serologic study was conducted with 3458 porcine serum samples randomly obtained from January 2014 to August 2016. The samples were tested with a blocking enzyme-linked immunosorbent assay developed in our laboratory. The seroprevalence was 33.3% in the complete sample set and was 25.9%, 37.8%, and 37.8% in 2014, 2015, and 2016, respectively. The seroprevalence was distinctly higher in summer (39.9%) and autumn (42.0%) than in spring (28.9%) and winter (23.3%). Shanghai was the region with the highest seroprevalence (54.2%) and Jiangsu the region with the lowest (23.0%). The seroprevalence was markedly higher in boars (47.1%), multiparous sows (47.0%), and replacement gilts (39.2%) than in piglets (24.2%), fattening pigs (17.2%), and nursery pigs (12.5%). These data demonstrate that the prevalence of M. suis infection is increasing yearly in eastern China.
اظهر المزيد [+] اقل [-]Temporal and spatial dynamics of porcine reproductive and respiratory syndrome virus infection in the United States النص الكامل
2015
OBJECTIVE To measure incidence and estimate temporal and spatial dynamics of porcine reproductive and respiratory syndrome virus (PRRSV) infection in US sow herds. ANIMALS 371 sow herds in the United States from 14 production companies. PROCEDURES The exponentially weighted moving average was used to monitor incident PRRSV infections for onset of an epidemic. The spatial scan statistic was used to identify areas at significantly high risk of PRRS epidemics. A χ2 test was used to estimate whether there were significant differences in the quarterly and annual PRRS incidence among time periods, and a bivariable logistic regression model was used to estimate whether PRRSV infection during a given year increased the odds of that herd being infected in the following year. RESULTS During the 4-year period of this study, 29% (91/319; 2009 to 2010), 33% (106/325; 2010 to 2011), 38% (135/355; 2011 to 2012), and 32% (117/371; 2012 to 2013) of the herds reported new infections. Weekly incidence was low during spring and summer and high during fall and winter. The exponentially weighted moving average signaled the onset of a PRRSV epidemic during the middle 2 weeks of October each year. Disease incidence was spatially clustered. Infection in the previous year increased the odds of infection in 2010 to 2011 and 2011 to 2012. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated a striking repeatability in annual PRRSV temporal and spatial patterns across 4 years of data among herds from 14 production companies, which suggested that efforts to control PRRSV at a regional level should continue to be supported.
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