خيارات البحث
النتائج 301 - 310 من 693
Detection of native interferon-γ in nyala (Tragelaphus angasii): Towards diagnosing tuberculosis النص الكامل
2019
Lezaan Roux | Alicia J. McCall | Anita L. Michel
Mycobacterium bovis is the main cause of tuberculosis in wildlife. In South Africa, African buffaloes (Syncerus caffer) are a wildlife maintenance host while a number of other species are considered spillover hosts. Nyala (Tragelaphus angasii), a large antelope species from Southern Africa, is frequently traded and can be infected with M. bovis. Interferon gamma (IFN-γ) release assays that detect cell-mediated immune (CMI) responses to M. bovis infection have shown promise in elephants, rhinoceroses and buffaloes. The BOVIGAM® assay is a commercial IFN-γ release assay designed to detect tuberculosis in cattle and has been validated in buffaloes. We tested the suitability of the BOVIGAM® assay to detect native IFN-γ release in nyala. Blood samples collected from 17 nyalas were stimulated with different mitogens and IFN-γ release measured. We found that incubating whole blood with phorbol 12-myristate 13-acetate and calcium ionophore (PMA/CaI) resulted in the highest levels of IFN-y release. Samples stimulated with tuberculin purified protein derivatives of M. bovis (PPDb) and M. avium (PPDa) did not show significant IFN-γ production. An intradermal tuberculin test (IDT) and culture of tissues from 15 of the 17 culled nyala were also performed, which supported the findings of the BOVIGAM® assay, suggesting the potential value of this assay for the diagnosis of tuberculosis in nyala.
اظهر المزيد [+] اقل [-]Seasonal activity of ticks infesting domestic dogs in Bejaia province, Northern Algeria النص الكامل
2019
Rosa Kebbi | Mohamed Nait-Mouloud | Lila Hassissen | Abdelhanine Ayad
Seasonal activity of ticks infesting domestic dogs in Bejaia province, Northern Algeria النص الكامل
2019
Rosa Kebbi | Mohamed Nait-Mouloud | Lila Hassissen | Abdelhanine Ayad
This epidemiological study aimed to determine the species of tick infestation in dogs, their prevalence and dynamic in the Bejaia province, northeastern Algeria. A total of 631 dogs were examined from different localities of the Bejaia province between March 2016 and February 2017. Of the 631 examined dogs, 15% were infested with one or more tick species. A total of 339 adult ticks were collected and identified, including 199 male tick species and 140 female tick species. Our results revealed that most of these were Rhipicephalus species, with Rhipicephalus sanguineus (51.32%) being the most prevalent followed by Rhipicephalus bursa (35.1%) and Rhipicephalus turanicus (12.98%). Ixodes ricinus represented only 0.6% of all ticks collected. The highest infested seasons were spring (22.55%) and summer (22.54%) and the lowest infested seasons were autumn (8.62%) and winter ( 0.9%). There is no significant difference between the sex of the animal and the prevalence of infestation (p = 0.837). Also, the prevalence of infestation by ticks in young animals was higher than that in adult animals (p = 0.550). A significant difference between the prevalence of infestation and animal breed was observed (p = 0.042). This study is the first epidemiological investigation conducted on the prevalence of hard ticks infesting domestic dogs in Bejaia (northeastern Algeria) based on conventional methods. It is therefore necessary to implement an effective tick control strategy during infestation periods in order to prevent vector-borne diseases.
اظهر المزيد [+] اقل [-]Seasonal activity of ticks infesting domestic dogs in Bejaia province, Northern Algeria النص الكامل
2019
Kebbi, Rosa(University of Bejaia Faculty of Nature and Life Sciences Department of Environment Biological Sciences) | Nait-Mouloud, Mohamed(University of Bejaia Faculty of Nature and Life Sciences Department of Environment Biological Sciences) | Hassissen, Lila | Ayad, Abdelhanine(University of Bejaia Faculty of Nature and Life Sciences Department of Environment Biological Sciences)
This epidemiological study aimed to determine the species of tick infestation in dogs, their prevalence and dynamic in the Bejaia province, northeastern Algeria. A total of 631 dogs were examined from different localities of the Bejaia province between March 2016 and February 2017. Of the 631 examined dogs, 15% were infested with one or more tick species. A total of 339 adult ticks were collected and identified, including 199 male tick species and 140 female tick species. Our results revealed that most of these were Rhipicephalus species, with Rhipicephalus sanguineus (51.32%) being the most prevalent followed by Rhipicephalus bursa (35.1%) and Rhipicephalus turanicus (12.98%). Ixodes ricinus represented only 0.6% of all ticks collected. The highest infested seasons were spring (22.55%) and summer (22.54%) and the lowest infested seasons were autumn (8.62%) and winter ( 0.9%). There is no significant difference between the sex of the animal and the prevalence of infestation (p = 0.837). Also, the prevalence of infestation by ticks in young animals was higher than that in adult animals (p = 0.550). A significant difference between the prevalence of infestation and animal breed was observed (p = 0.042). This study is the first epidemiological investigation conducted on the prevalence of hard ticks infesting domestic dogs in Bejaia (northeastern Algeria) based on conventional methods. It is therefore necessary to implement an effective tick control strategy during infestation periods in order to prevent vector-borne diseases.
اظهر المزيد [+] اقل [-]THE EFFECTS OF 4̄,4 ̄̄ -(4,5,6,7-TETRAHYDRO- [1,2,3-] SELENADIAZOLO [4,5E] PYRIDINE-4,6-DIYL) BIS(BENZENE-1,3-DIOL) ON FERTILITY, REPRODUCTIVE HORMONES AND OVARIAN HISTOLOGICAL CHANGES IN FEMALE RATS TREATED WITH DIPYRONE. النص الكامل
2019
The current study aimed to evaluate the effects of [ 4’,4” -(4,5,6,7-tetrahydro- [1,2,3-] selenadiazolo[4,5e] pyridine-4,6-diyl) bis(benzene-1,3-diol)] (T) on female fertility and reproductive hormones, in addition histopathological examination of ovaries in comparison with the effects of Metamizole or Dipyrone (Di). Four groups of mature female rats each group consist of ten healthy female rats. Three groups received T and /or Di dissolved in drinking water 2mL (DW), the forth group received 2 mL of (DW) for 20 days before mating and ten days after mating. The results indicated that there was a significant increase in the number of births of (T) group (13.70±3.34) compared to DW group (9.7±1.06). Also, significant decline in T&Di group to (6.91±0.32), with no pregnancies reported in Di treated group. The pregnancy percentage was in T&Di group basically decline to 40% compare with T and DW groups 100%. Follicle stimulating Hormone (FSH) level showed a significant elevation (p<0.05) in T group (5.19mIU/mL ±0.72), and T&Di (5.12 mIU/mL ±0.78) compared with DW (1.61mIU/mL±0.52), and Di (1.46 mIU/mL ±0.60) groups. LH concentration of T (1.88 mIU/mL ±0.48), and T&Di (1.67 mIU/mL ±0.41) groups LH values than in DW (1.75 mIU/mL ±0.67). Only in Di group (0.88 mIU/mL ±0.48) LH value reduced significantly (p<0.05) than in other test groups. T group (17.22 mIU/mL ±4.50) progesterone level; also T&Di group progesterone level (10.11 mIU/mL ±2.05) statistically important increase, while Di group there is essentially decreased (p<0.05) to (2.69 mIU/mL±0.89) compare to DW (6.31 mIU/mL ±1.41). Histopathological results showed that ovarian section of Di group disclosed large cystic corpus luteum CC.L, absence of Graafian Follicles and follicles at different stages of development. T group section showed normal Graafian follicles and different follicles in developmental stages, as well as there were several persistence corpus luteum. Ovary of T&Di female rats relieves improvement of some Graafian follicles, Cystic corpus luteum (CC.L). Furthermore, there was some of clear C.L. It can be concluded that T compound had a Good effect on the level of reproductive hormones and increase fertility in female rats. While Di long term treatment had bad effects on female fertility, by affecting reproductive hormones levels and pathological change of ovaries.
اظهر المزيد [+] اقل [-]HISTOPATHOLOGICAL STUDY OF PLATELETE RICH PLASMA FOR TREATMENT OF INDUCED CUTANEOUS ULCER IN DIABETIC RABBITS INDUCED BY STREPTOZOTOCIN النص الكامل
2019
The present study investigates the effect of platelets rich plasma (PRP) on the healing of skin ulcers in rabbits in which diabetes mellitus (DM) was induced using Streptozotocin. The study was applied on 12 adult rabbits of both sexes with a mean weight of 1000-1500 g. They were divided randomly into two equal groups (six rabbits per group). DM was induced in two groups by injected Streptozotosin 65 mg / kg B.W IV. Ulcers were made in 3 cm length and 2 cm width in all rabbits in the gluteal region under the general Anesthesia by mixture of xylazine hydrochloride and ketamine hydrochloride in sterile conditions. The first group was used as control; the second group was treated with platelets rich plasma on the site of ulcers. The first group in the 7 days, showed large ulcers and pus; in the 14 days, were ulcers continued with thickened epidermal. The second group, in the 7days, showed skin ulcers with dermal thickening and a beginning of skin formation. In the 14th days, the treated group showed small ulcer remaining with dermal fibrosis.ELISA results have been shown the effect of platelets rich plasma on tumor necrosis factor-alpha (TNFα) concentration in the blood after 10 days of skin lesions inducing. The natural concentration of TNFα is 6.4 pg/ml in range (4.2-7.9) pg/ml. The first group had a concentration below 5.3 pg/ml, due to the DM. The second group was highly concentrated in 10.4 pg/ml. The results of the histopathological and ELISA confirmed that there are marked healing and elevating TNFα concentration in the treated group.
اظهر المزيد [+] اقل [-]ABILITY OF Leishmania donovani TO CONGENITAL TRANSMISSION IN EXPERIMENTAL BALB/C MICE النص الكامل
2019
The aim of the current study was to investigate the ability of the transmission of Leishmania donovani parasite from pregnant mothers to their embryos in experimental BALB/c mice. After 5 days of copulation males with females,females were injected with 1012 parasite/ml of promastigote stage through the tail vein, which is grown in NNN-medium. This study demonstrated parasite ability to congenital transmission from mothers to their embryos by placenta through the appearance of amastigote in tissue sections of liver and bone marrow of infected mice fetusescompared with uninfected fetuses. I
اظهر المزيد [+] اقل [-]EFFECT OF FEEDING OF HEAD LETTUCE AND ALFALFA ON GROWTH PERFORMANCE AND REPRODUCTIVE PARAMETERS IN ADULT MALE RABBITS النص الكامل
2019
The present objective was done for investigate the ameliorative feeding effect of Lactuca sativa and alfalfa on growth performance and reproductive parameters (concentration of hormones, characteristic of sperm epididymal in adult male rabbits). Twelve adult male rabbits were divided randomly in to two groups. Control group (G1) animals feeding on alfalfa (1kg/day/animal) for 6 weeks. Group Two (G2) this group feeding on head lettuce (1kg/day/animal) for 6 weeks. The result revealed significantly increase in serum testosterone, LH and FSH concentration and significantly differences were observed in sperm concentration, viability, abnormality and decrease significantly in motility of sperm in group feeding on head lettuce compared to the group feeding on alfalfa.
اظهر المزيد [+] اقل [-]ASSOCIATION OF POLYMORPHISMS IN SLC11A1 GENE WITH AUTOIMMUNITY CAUSED BY Mycobacterium avium subspecies paratuberculosis(MAP) IN CATTLE. النص الكامل
2019
Johne's disease is one of the main causes of economic losses in ruminants and a major health hazard in the developed and developing developed countries.In this study, PCR detection of insertion sequences IS900 of MAP in the buffy coat of cows (n = 81), of this 29 (35.8%) cow showed positive results. By Restriction fragment length polymorphism (PCR-RFLP),two single nucleotide polymorphisms (SNPs) of SLC11A1 gene were tested for finding their association with susceptibility to bovine Johens disease in Iraqi cattle. A total of 50 cows were tested, theirresult revealedthat at rs109453173 locus twoelectromorph‘CC’ (374 bp) and ‘CG’ (374, 293 and 81 bp).The rs109915208 locus also showed two electromorph, ‘TT (344bp) and ‘CT’ (344, 215 and 129 bp) . The differences in the electromorph between IS900 positive and negative cows were found to be statistically significant (p = 0.0031). No significant difference in these electromorph at SNP locus rs109915208 between IS900 positive and negative cows. Out of two SNPs from SLC11A1 gene, rs109453173 had a significant association with the susceptibility to Johne's disease. The CC’ electromorph observed at rs109453173 locus showed a significant association with the susceptibility to bovine paratuberculosis in cows. The OR of ‘CC’ in‘IS900 positive versusIS900 negative cattle was7.8750, suggesting that cows having ‘CC’ electromorph were susceptible to Johne's disease compared to ‘CG’ electromorph .
اظهر المزيد [+] اقل [-]EVALUATION ROLE OF THE AUTOGENOUS PERITONEUM AS A SCAFFOLDS ON THE HEALING OF ACHILLES TENDONS IN DOGS النص الكامل
2019
A H Allawi | L M Alkattan
The objective of the current study was to evaluate the healing of experimentally severed Achilles tendons of the dogs repaired by Kessler suture pattern alone or with autologous peritoneal scaffold. A total of twelve healthy, neurologically and orthopedically local breed dogs were used in this study, which were randomly allocated into two equal groups (6 of each). The 1st group as a control, while the 2nd as a treated group. In both groups the Achilles tendon of one hind limb was completely severed transversely, in 1st group the tendon was repaired by Kessler suture pattern using silk No.3, while in the 2nd group a combination of Kessler suture and autogenous peritoneal scaffold was applied to reinforce the tendon repair. The site of surgery was evaluated clinically, while gross and histological study was performed at 15, 30 and 60 days post-operative days. Results was revealed that autologous peritoneum graft has a remarkable effects on Achilles tendon healing represented by improving fibroblastic proliferation, reduce adhesion, increase tendon strength and improve tendon function when compared with the control group. We concluded that peritoneal graft application has valuable and beneficial effects on treatment of ruptured Achilles tendon in dogs when utilizing it in conjunction with the traditional procedures.
اظهر المزيد [+] اقل [-]In vitro evaluation of negative pressure generated during application of negative suction volumes by use of various syringes with and without thoracostomy tubes النص الكامل
2019
Mezzles, Marguerite J. | Murray, Rebecca L. | Heiser, Brian P.
OBJECTIVE To determine the amount of negative pressure generated by syringes of various sizes with and without an attached thoracostomy tube and whether composition of thoracostomy tubes altered the negative pressure generated. SAMPLE Syringes ranging from 1 to 60 mL and 4 thoracostomy tubes of various compositions (1 red rubber catheter, 1 polyvinyl tube, and 2 silicone tubes). PROCEDURES A syringe or syringe with attached thoracostomy tube was connected to a pneumatic transducer. Each syringe was used to aspirate a volume of air 10 times. Negative pressure generated was measured and compared among the various syringe sizes and various thoracostomy tubes. RESULTS The negative pressure generated decreased as size of the syringe increased for a fixed volume across syringes. Addition of a thoracostomy tube further decreased the amount of negative pressure. The red rubber catheter resulted in the least amount of negative pressure, followed by the polyvinyl tube and then the silicone tubes. There was no significant difference in negative pressure between the 2 silicone tubes. The smallest amount of negative pressure generated was −74 to −83 mm Hg. CONCLUSIONS AND CLINICAL RELEVANCE Limited data are available on the negative pressure generated during intermittent evacuation of the thoracic cavity. For the present study, use of a syringe of ≥ 20 mL and application of 1 mL of negative suction volume resulted in in vitro pressures much more negative than the currently recommended pressure of −14.71 mm Hg for continuous suction. Additional in vitro or cadaveric studies are needed.
اظهر المزيد [+] اقل [-]Effect of orally administered melatonin on intraocular pressure of ophthalmologically normal dogs النص الكامل
2019
Visser, Hannah E. | Dees, D Dustin | Millichamp, Nicholas J. | Vallone, Lucien V. | Scott, Erin M.
OBJECTIVE To determine the effect of orally administered melatonin on the intraocular pressure (IOP) of ophthalmologically normal dogs. ANIMALS 20 ophthalmologically normal dogs (40 eyes). PROCEDURES In a randomized crossover study, each dog received a 7-day regimen of melatonin (0.1 to 0.2 mg/kg, PO, q 12 h) and a placebo (150 mg of lactose powder in a capsule, PO, q 12 h), with a 7-day washout period between treatment regimens. Rebound tonometry was used to measure the IOP in both eyes of each dog 5 times at 2-hour intervals on days 0 (before administration of the first dose), 2, 4, and 7 (after administration of the last dose) of each treatment period. Repeated-measures ANOVA was used to evaluate the effects of treatment, day, and IOP measurement time within day on IOP. RESULTS Intraocular pressure was not significantly associated with treatment but was associated with day and the interaction between day and IOP measurement time within day. The mean ± SD IOP was 14.26 ± 2.95 and 14.34 ± 2.69 mm Hg for the melatonin and placebo regimens, respectively. Within each treatment period, the mean IOP tended to decrease from day 0 to 7 as well as within each day, which was attributed to the dogs becoming acclimated to the study protocol and natural diurnal variations in IOP. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that oral administration of melatonin (0.1 to 0.2 mg/kg, q 12 h for 7 d) did not significantly affect the IOP of ophthalmologically normal dogs.
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