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Prevalence and Antibiotic Susceptibility of Salmonella species Isolated from Beef and its Related Samples in Techiman Municipality of Ghana النص الكامل
2015
Frederick Adzitey | Joseph Kwame Nsoah | Gabriel Ayum Teye
Prevalence and Antibiotic Susceptibility of Salmonella species Isolated from Beef and its Related Samples in Techiman Municipality of Ghana النص الكامل
2015
Frederick Adzitey | Joseph Kwame Nsoah | Gabriel Ayum Teye
Salmonella species are important foodborne pathogens that have been implicated in a number of foodborne outbreaks. A total of 240 beef and its related samples obtained from the Techiman Municipality of Ghana were screened using the conventional method in the Food and Drug Administration-Bacteriological Analytical Manual (FDA-BAM)-USA. The overall prevalence of Salmonella species was 57.08%. The prevalence of Salmonella species ranged from 33.30% to 75.00% in the meat and its related samples (table, knife and apron), and 6.30% to 93.80% at the various meat sale points (locations where meat is sold). Prevalence of Salmonella species was significantly higher in meat (75.00%), table (60.00%) and knife (60.00%), than apron (33.33%). The most contaminated meat sale point was Kenten and Main markets (93.75%) and the least contaminated sale point was Sansema junction (6.25%). All the Salmonella species were susceptible to ciprofloxacin but resistant to vancomycin. Intermediate resistances occur for all the antibiotics except ciprofloxacin and vancomycin. The Salmonella species also exhibited 23 antibiotic resistant patterns with the pattern EVa (erythromycin and vancomycin) being the commonest (exhibited by nine different isolates). Multiple antibiotic index (MAR index) ranged from 0.11 to 0.67. This study revealed that beef and its related samples in Techiman markets are contaminated with Salmonella.
اظهر المزيد [+] اقل [-]Ciclo de vida, hábitos y enemigos naturales de Neoleucinodes elegantalis (Guenée, 1854), (Lepidoptera: pyralidae), pasador del fruto del lulo Solanum quitoense Lam. en el Valle del Cauca النص الكامل
1991
Muñoz L., Edilberto | Serrano P., Alberto | Pulido, Jaime I. | De la Cruz L., Jaime
Life cycle and habits studies of Neoleucinodes elegantalis under laboratory and field conditions. The life cycle under laboratory conditions (24°C, 74% RH) were for the egg, larvae and pupae 5.86; 24.58 and 12.33 days respectively. Longevity was 6.83 days for the female and 4.00 days for the male. Larva showed five instars each one with a duration of 3.58; 3.33; 4.16, 4.45 and 8.70 days. Under field condition (2100 m, 17C, + 80% RH), larva stage reached and average of 26 days. Oviposition in seven days reached an average at 93 eggs. Parthenogenesis was not observed. Natural enemies: Encyrtidae, possibly Copidosoma, parasiting egg and hatching in larvae at the last instar; 2.500 observed showed 1.6% parasitism Tachinidae 0.08% parasitism 527 pupae were observed showing 0.38% parasitism for Ichneumonidae. 400 pupae were observed showing 55% parasitism for Beauveria. Eggs laid on L. sculentum showed 81.93% parasitism for Trichogramma sp. | El cilo de vida de N. elegantalis en condiciones de laboratorio (ICA-Palmira, 1006 msnm., 24°C y 74% HR) para los estados de huevo, larva y pupa fue de 5.8, 24.58, 12.33 días en promedio; la longevidad de la hembra y del macho fue de 6.83 y 4.00 días. La larva no se alimentó del corión y presentó cinco instares (5.58, 3.333,4.416,4.450 y 8.70 días). En condiciones de campo (pradera, 2.100 m, 17C y + 80% HR) solo se pudo determinar la duración del estado de larva (26 días en promedio). La oviposición en 7 días fue de 93 huevos; no se presentó partenógenesis. Se encontraron los siguientes enemigos naturales: Encyrtidae, posiblemente Copidosoma sp, parásito de huevos que emerge en larvas de último instar; en 2.500 larvas evaluadas presentó un parasitismo de 1.6%. Un Tachinidae parasitó 0.08% de 2.500 larvas evaluadas. Un Ichneumonidae, parasitó 0.38% de 527 pupas evaluadas. Un entomopatógenos, posiblemente Beauveria, parasitó el 55% de 400 pupas evaluadas. Se registró Chrysopa predatando huevos. En posturas colocadas sobre tomate de mesa se evaluó un parasitismo del 81.93% por Trichograrnma spp.
اظهر المزيد [+] اقل [-]St John’s Wort (Hypericum perforatum L.) in Broiler Nutrition النص الكامل
2024
Özgün Işık | Helin Atan | Figen Kırkpınar | Ayşe Betül Avcı
Nowadays, the concept of sustainability is important in poultry meat production as in every field. The health status of the birds must be well-stated for sustainable broiler production. Considering that synthetics attract reactions and some of them are banned or limited, the use of natural feed additives for health protection has been the focus of research attention. St John’s wort is a plant that can positively affect the health of animals with bioactive components such as the hypericin it contains. St John’s wort or hypericum (Hypericum perforatum L.) has antiviral and antimicrobial effects in broilers and positively affects on blood biochemical parameters. This positive effect on health level also improves performance. The review aims to give information about St John’s wort and to examine a limited number of studies on their use in the diet of broilers.
اظهر المزيد [+] اقل [-]Prevalence and Antimicrobial Susceptibility of Escherichia Coli O157 Isolated From Raw Milk Marketed in Chittagong, Bangladesh النص الكامل
2017
Md. Kauser-Ul Alam | Shireen Akther | Nazmul Sarwar | Shamsul Morshed | Goutam Kumar Debnath
Escherichia coli is an emerging public health concern in most countries of the world. It is an important cause of food-borne human disease. The present study assessed the prevalence and determined the antibiotic resistance patterns of E. coli from raw milk marketed in Chittagong, Bangladesh. Of the raw milk marketed in Chittagong ~33(18%) of the 186 raw milk samples of it contains E. coli, indicator bacteria for any enteric pathogens. The mean viable count of total bacteria was 4.04×108 cfu/ml and the mean viable count of E. coli in the contaminated raw milk was 1.88×106 cfu/ml. E. coli from only six (18.2%) of the 33 positive samples yielded colourless colonies across the CT-SMAC, suggesting the probable presence of populations belonging to the serotype O157 and rest of the isolates 27 (81.82%) produced coloured colony on CT-SMAC considering the probable presence of populations belonging to the serotype non-O157. Growth of probable E. coli O157, as evidenced by the colourless colonies on CT-SMAC compared to coloured colonies from other bacteria. Confirmed isolates were further subjected to antimicrobial susceptibility test using the Agar disc diffusion technique. Antibiotics susceptibility profile showed that all the isolates in case of E. coli O157, penicillin (100%), tetracycline (100%), amoxicillin (83.33%) and erythromycin (83.33%) were the most resistant whereas ciprofloxacin (66.67%), gentamicin (50.0%), and streptomycin (50.0%) were the most sensitive antibiotics. In case of E.coli non-O157 susceptibility profile showed that chloramphenicol (40.74%), erythromycin (40.74%) and oxacillin (37.04%) were the most resistant whereas ciprofloxacin (70.37%), sulphamethoxazole/trimethoprim (S/T) (59.26%) and gentamycin (55.55%) were the most sensitive antibiotics. The antimicrobial resistance exhibited by E. coli O157and non-O157 strains in this study is an indication of possible antibiotic abuse.
اظهر المزيد [+] اقل [-]Prevalence and Antimicrobial Susceptibility of Escherichia Coli O157 Isolated From Raw Milk Marketed in Chittagong, Bangladesh النص الكامل
2017
Md. Kauser-Ul Alam | Shireen Akther | Nazmul Sarwar | Shamsul Morshed | Goutam Kumar Debnath
Escherichia coli is an emerging public health concern in most countries of the world. It is an important cause of food-borne human disease. The present study assessed the prevalence and determined the antibiotic resistance patterns of E. coli from raw milk marketed in Chittagong, Bangladesh. Of the raw milk marketed in Chittagong ~33(18%) of the 186 raw milk samples of it contains E. coli, indicator bacteria for any enteric pathogens. The mean viable count of total bacteria was 4.04×108 cfu/ml and the mean viable count of E. coli in the contaminated raw milk was 1.88×106 cfu/ml. E. coli from only six (18.2%) of the 33 positive samples yielded colourless colonies across the CT-SMAC, suggesting the probable presence of populations belonging to the serotype O157 and rest of the isolates 27 (81.82%) produced coloured colony on CT-SMAC considering the probable presence of populations belonging to the serotype non-O157. Growth of probable E. coli O157, as evidenced by the colourless colonies on CT-SMAC compared to coloured colonies from other bacteria. Confirmed isolates were further subjected to antimicrobial susceptibility test using the Agar disc diffusion technique. Antibiotics susceptibility profile showed that all the isolates in case of E. coli O157, penicillin (100%), tetracycline (100%), amoxicillin (83.33%) and erythromycin (83.33%) were the most resistant whereas ciprofloxacin (66.67%), gentamicin (50.0%), and streptomycin (50.0%) were the most sensitive antibiotics. In case of E.coli non-O157 susceptibility profile showed that chloramphenicol (40.74%), erythromycin (40.74%) and oxacillin (37.04%) were the most resistant whereas ciprofloxacin (70.37%), sulphamethoxazole/trimethoprim (S/T) (59.26%) and gentamycin (55.55%) were the most sensitive antibiotics. The antimicrobial resistance exhibited by E. coli O157and non-O157 strains in this study is an indication of possible antibiotic abuse.
اظهر المزيد [+] اقل [-]Prevalence and Antibiotic Susceptibility of Salmonella species Isolated from Beef and its Related Samples in Techiman Municipality of Ghana النص الكامل
2015
Frederick Adzitey | Joseph Kwame Nsoah | Gabriel Ayum Teye
Salmonella species are important foodborne pathogens that have been implicated in a number of foodborne outbreaks. A total of 240 beef and its related samples obtained from the Techiman Municipality of Ghana were screened using the conventional method in the Food and Drug Administration-Bacteriological Analytical Manual (FDA-BAM)-USA. The overall prevalence of Salmonella species was 57.08%. The prevalence of Salmonella species ranged from 33.30% to 75.00% in the meat and its related samples (table, knife and apron), and 6.30% to 93.80% at the various meat sale points (locations where meat is sold). Prevalence of Salmonella species was significantly higher in meat (75.00%), table (60.00%) and knife (60.00%), than apron (33.33%). The most contaminated meat sale point was Kenten and Main markets (93.75%) and the least contaminated sale point was Sansema junction (6.25%). All the Salmonella species were susceptible to ciprofloxacin but resistant to vancomycin. Intermediate resistances occur for all the antibiotics except ciprofloxacin and vancomycin. The Salmonella species also exhibited 23 antibiotic resistant patterns with the pattern EVa (erythromycin and vancomycin) being the commonest (exhibited by nine different isolates). Multiple antibiotic index (MAR index) ranged from 0.11 to 0.67. This study revealed that beef and its related samples in Techiman markets are contaminated with Salmonella.
اظهر المزيد [+] اقل [-]