Secondary metabolites, gaining importance in pharmaceutical, cosmetic, perfumery, food industry and agrarian struggle, are synthesized in different organs such as root, leaves, shoot and seed in plants. These compounds are defined as “light in bulk” because of the low synthesis rate but “high in value” because of the wide range of applications, activities and economic values. Obtaining of the secondary metabolites found in roots by conventional methods is based on dismantling of these plants from the nature or the cultural field and isolating by the different methods. Detachment of plants from nature causes the loss of genetic resources. And it has some difficulties as the challenges and differences in terrain and climate conditions, low metabolite yield and quality and more labor. Thus a new approaches is needed to enable more economic, higher metabolite yield and quality compared to the conventional methods. Therefore, in vitro techniques have gained importance. With this review, it was aimed to inform in vitro applications used to increase root-related secondary metabolites production in order to guide future researches.

Honey is a natural substance produced by honeybees (Apis mellifera L.) from the nectar of blossoms or from secretions of living parts of plants or excretions of plant sucking insects on the living parts of plants, which honeybees collect, transform and combine with specific substances of their own, store and leave in the honey comb to ripen and mature. Besides being of carbohydrate-rich food, honey has been used as a functional food for its potential health benefits. To explain how different kinds of carbohydrate-rich foods directly affect blood sugar, the researchers developed the concept of the “glycemic index” (GI) that ranks carbohydrates on a scale based on how quickly and how much they raise blood sugar levels after eating. The diet should include adequate and healthy balance of nutrients, and according to many health professionals the concept of GI provides a useful means of selecting the most appropriate carbohydrate containing foods for the maintenance of health and the treatment of several disease states. There have been some studies on determining the GI of honey. Further more, we need to determine the GI of various honey types with different botanical and geografical origin. Researches on the issue will serve to bring awareness in the public consciousness.

The aim of the study was to find the diversity index of flora, investigation of species at pond area and biomass calculation of economic plants at Chashma Barrage, Pakistan. The study area consisted of river Bella, pond area and eastern edge of river bed near Ali Wali Ghandi village at Chashma Barrage. The seasonal variation in the abundance of floral species was also related with the increase in temperature. Cyperus deformus, Phragmites kerka, Saccharum spontaneum were most abundant in the river ‘Bella’ habitat. Persicassia amphibian, Cyperus deformus and Polygonum royleanum were abundant in Aliwali Ghandi habitat. Aquatic plants of families Potamogetonaceae, Nymphaeaceae, Najadaceae and Hydrocharitaceae were most common in pond area . The ecotones were wide and variable between the aquatic biotopes and the river bed biotopes. On the Aliwali Ghandi site the moist soil with puddles and small channels of water were found and Cyperus deformus biotope prevailed. In the river ‘Bella’ site Phragmites, Sacharum was the biotope and in water Hydrilla biotope was recorded. An increase in biomass of Phragmites, Sacharum and Typha was recorded with the passage of time. The species of the area have significant importance in socio economics of the local community of Chashma Barrage.

Fruit and vegetables are an important component of a healthy diet and the main antioxidant suppliers in the human diet. Consumption of foods derived from fruits and vegetables is also essential; fruit juices, ciders, wines, and vinegars also contain significant amounts of polyphenolic compounds. The aim of the study was to determine the effect maceration of antioxidant activity and phenolic content of apple cider. Red delicious apples were used to produce natural apple cider with and without inclusion of maceration. Samples were taken from fresh red apple juice, macerated samples and apple cider. Apple cider (maceration was applied) (CAM) had the highest total phenolic content, chlorogenic acid, ORAC and TEAC levels. Chlorogenic acid was the dominant phenolic substance in apple juice and cider samples and chlorogenic acid was increased with maceration process.

Pinus brutia Ten., Red pine, known to be tough drought resistant pine specie, could effectively be used for afforestation of disturbed areas. It is of great interest for the afforestation in arid zones. Appropriate seed sources for the specific areas guarantees reforestation success. Away from its native areas Pinus brutia Ten. is planted for its ornamental value and timber production purposes. Selection of drought resistant provenances can very well increase the survival success. In this study, the effects of water potential on germination were studied in fourteen provenances of Pinus brutia Ten. from Turkey. Water potentials between 0 and -8 bars were obtained using polyethylene glycol 6000 (PEG-6000) solutions. Seeds were kept for 35 day at 20 ± 0.5°C. A decrease in water potential produced a marked reduction in germination percentage and germination value. As a result, significant variations between the provenances were found. It was determined that, under a -8 bar water stress, Isparta-Bucak and Mersin-Silifke, respectively corresponding to 58% and 57% of the control group, were the least water stress affected provenances.

Shiga toxin (Stx)-producing E. coli (STEC) is the most important foodborne pathogen which is the causal agent of mild diarrhea, bloody diarrhea, hemolytic-uremic syndrome (HUS) in human. The present study was designed to determine the prevalence and identification of Shiga toxin (Stx)-producing E. coli in poultry, detection of its source of infection in poultry and transmission pattern to human. For this purpose a total of 150 samples (cloacal swab-60, feed -15, water-15 and egg -60) were collected and analyzed in bacteriology laboratory by cultured in different bacteriological media followed by gram’s staining, biochemical tests and Polymerase Chain reaction (PCR). The PCR was performed by targeting 16s rRNA gene and shiga toxin producing gene in E. coli. Out of 150 collected samples, E. coli was found in 81 (54%) samples. Presence of E. coli was 100% in both feed (n=15) and egg (n=60), whereas 10% in cloacal swab (n=6). Water samples were totally free of E. coli. The stx2 gene was detected in all samples whether all samples were negative for stx1 gene. The study revealed that, poultry feed acts as a source of E. coli infection in poultry, which may be transmitted to environment and human via meat or eggs. Antibiotic sensitivity test revealed that isolated bacteria were highly sensitive to Ciprofloxacin.

The objective of this study was to determine barning conditions of dairy farms registered to Cattle Breeders Association (CBA) Ödemiş district of İzmir. The 92 farms were selected among members of the CBA by proportional sampling method. The farms were divided into three groups according to the number of cattle: group 1 from 5 to 20, group 2 from 21 to 40 and group 3 with over 41 heads, respectively. The farms were divided into three groups according to the number of cattle: 5-20; 21-40 and over 41 heads, respectively. The research results indicated that 76.1% of the barns were shed type, 15.2% were corral, 8.7% were completely closed-up. The flooring types of these barns were a mixed of concrete and soil (73.9%), concrete (19.6%) and soil (6.5%). The stocking density for group 1, 2 and 3 were 22.2 m2, 19.5 m2 and 18.2 m2 per cow, respectively. The barns had a range of full cattle capacity from 12.5 m2 to 15 m2 per cow. But none of the farms surveyed did not use fully capacity of the barn. The present running capacity of farms were between 55% and 80%. It was observed that 19.6% of farms had a individual calf section, 82.6% had a night lighting at the barn, and 64.1% had a seperate concenrated feed store. All the farms were milked with milking machines. The percentage of farms with bucked milking machine was 47.8%, those with an air pipeline system was 37% and those with parlour system was 15.2%. None of farms had manure pit. Consequently, as herd size grow, the presence of free barn, calving pen, feed store and milk cooling tank increase. It can be concluded that improving the housing conditions is much more related with increasing the herd size per farm.

In this study, examined the structure and problems of fruit sapling producing enterprises and offers solutions in The Hatay Province. The data were obtained from 92 enterprises by the method of full count and face to face with producers. Of the 51.15% population in creating family business primary school graduates, the proportion of university graduates remained at 8.62%. The rate of agricultural engineer employment in the businesses is 2.18%. While 64.05% of the enterprisers the father's profession, this profession began taking 3.37's% of nursery education. Average potential family labour is the 4.22 male labour forces in enterprises. The family labour utilization rate was calculated as 50.71%. Of the 88.48% in fruit sapling businesses, 5.32's% in other agricultural productions and 5.22's% in non-agricultural jobs of total used men workforce. The businesses have average total land size of 25.74 acres which it has established 12 acres of fruit sapling production land. While 74.58% of the total fruit sapling land is property, 25.42's% is for rent situation. The operating capital is calculated as the average of 229.308 TL. The most common species of fruit sapling production were citrus (46.20%) and olive trees (35.29%). The problems faced by most of the farms in the region were high input prices, excessive bureaucratic procedures preventing memberships into the union, an inadequate amount of support provided by the state, production-threatening diseases of plants and difficulties in finding qualified staff in the region. The results show that enterprises need to increase their production capacity, to unite under one organization to facilitate the resolution of problems.

Concern over food authenticity has increased as a result of an increase in the consumption of processed foods containing meat or animal products. This raises a number of issues where the presence of pork in such foods is considered unacceptable in most Muslim and Jewish communities around the world. It also applied to the prohibition of beef consumption among Hindus. In order to ensure the absence of unwished meat products or mixing of meats from different sources in processed foods, a specific and sensitive test is essential. For this purpose we developed a molecular test based on DNA amplification by polymerase chain reaction (PCR) of the cytochrome b gene followed by reverse line blot analysis (RLB). Using this method many samples may be treated simultaneously and meat origins can easily be detected from processed foods or foods containing mixed meat sources; also, added pork components such as fat may be identified by this methodology. The PCR/RLB method is considered to be a sensitive and specific technique; it can detect one nucleotide change within the PCR-amplified DNA segment.

The factor affecting nutritive and feeding value of olive cake (OC) was evaluated and, some suggestion was made regarding using olive cake in ruminant nutrition. It is evaluated that the nutritive value of OC can be able to support maintenance requirement of ruminant, although its nutritive value is affected by different factors. However, when taking into consideration of expense needed for preservation of OC, it is wise to use OC in nutrition after de-stoning. The crude protein, ether extract, NDF, lignin and non-fiber carbohydrates content of partly de-stoned OC (POC) was determined as 75 (56-93), 126 (68-184), 571 (443-700), 222 (174-269) and 141 (55-227) g/kg dry matter (DM). These values show that POC is a valuable source of ruminant feed. Besides, high and quality ether extract content of OC separates it from other feed and made it a special feed. Treatment with alkali increases the degradability and in vivo digestibility parameters of OC. Therefore, ensiling allowing the treatment with additives is best option to preservation of OC. Even, additional extraction of oil from POC decreases its nutritive value; studies showed that POC after additional oil extraction has still favorable nutritive value. Studies showed no adverse effect when POC substitute 20% of ration of feedlot lamb. For dairy animal, it is evaluated using POC to provide 15-20 g/kg DM oil when it substitute of 10-20% of total ration allow to produce more milk with increased fat and quality. In conclusion, de-stoning process turns OC to a valuable feed source, allows reduce feed cost, and increase animal performance and product quality. However, because nutritive value of OC affecting from very different factors, it can be propose analyze its nutritive value before using it in ruminant nutrition.