Synthesis of substituted terpyridine ligands for use in protein purification

Lin, Chih-Pei; Florio, Pas; Campi, Eva M.; Zhang, Chunfang; Fredericks, Dale P.; Saito, Kei; Jackson, W Roy; Hearn, Milton T.W.

Synthesis of substituted terpyridine ligands for use in protein purification

2014

A number of 4,4″-disubstituted terpyridines bearing a 4′-thioethanamine linker, and regioisomers thereof, have been synthesised from 4-substituted picolinates. The substituted terpyridines were immobilised onto epoxy-activated Sepharose™ FF gel, creating functionalised solid supports for the fractionation of proteins—including antibodies—by mixed mode affinity chromatography. The metal chelating properties of the immobilised ligand render the stationary phase also amenable for use in immobilised metal-ion affinity chromatography (IMAC), and have been demonstrated with the purification of suitably tagged green fluorescent protein (GFP).

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AGROVOC Keywords

chemical reactions chemical structure fractionation gels ligands organic compounds

Bibliographic information

Published in

Tetrahedron

Volume 70 Pagination 8520 - 8531 ISSN 0040-4020

Publisher

Elsevier Ltd

Other Subjects

Gspwtgmnowdxds-uhfffaoysa-n; Jszdthvzlgmcck-uhfffaoysa-n; Xzfjppvfyqscqj-uhfffaoysa-n; Jeqknlrrpzdfpr-uhfffaoysa-n; Fqyyipzpelsldk-uhfffaoysa-n; Green fluorescent protein; Sfjkbjngansoqy-uhfffaoysa-n; Itwdknozjqwojy-uhfffaoysa-n; Wtecicqcojdrad-uhfffaoysa-n; Pmtbmmjmemnufq-uhfffaoysa-n; Vmyboinexgmqql-uhfffaoysa-n; Pxjvlwhtlmyebz-uhfffaoysa-n; Cikkfgbrpgnrmr-uhfffaoysa-n; 4′-halo-terpyridines; Lcenywxxecmymi-uhfffaoysa-n; Dphrwlzxobewmi-uhfffaoysa-n; Qcbdnddaqitxsv-uhfffaoysa-n; Affinity chromatography; Vavwnmrtafgxdl-uhfffaoysa-n; Ojdkengkkyvjly-uhfffaoysa-n; Jmhkkvcrihpidq-uhfffaoysa-n; Ozrrttujphvvrm-uhfffaoysa-n; Neqhyfprlitikv-uhfffaoysa-n; 4′-terpyridones; Qrwdrnlaijagrh-uhfffaoysa-n; Xlfoqlxkqvmxoi-uhfffaoysa-n; Vtenwipswampki-uhfffaoysa-n; Ahemfmcebijrmu-uhfffaoysa-n; Ngjfomvuanvaeo-uhfffaoysa-n; Protein purification; Irpvesufyprtlg-uhfffaoysa-n; Juagwhxwlxigpq-uhfffaoysa-n; Lphkjbaiundtrm-uhfffaoysa-n; Atvhawnfngfpem-uhfffaoysa-n; Iwlvxcyiqfrsoy-uhfffaoysa-n; Cpbotxgxhpdxbz-uhfffaoysa-n; Affinity chromatography; Cnrzqeflvzrgju-uhfffaoysa-n; Irfjmkccfgxsdk-uhfffaoysa-n; Sxczkmxrgzmsmh-uhfffaoysa-n; Mqipwkkuzfxneo-uhfffaoysa-n; Wcefpnbfmyhrfk-uhfffaoysa-n; Lqkdqblphuibid-uhfffaoysa-n; 4″-disubstituted-4′-aminoethanesulphanylterpyridines; Kzjyxksrukrotc-uhfffaoysa-n; Heterocyclic compound synthesis; Uxcbowmrkbjegr-uhfffaoysa-n; Syyzsllicdzuek-uhfffaoysa-n; Qhzgjqwangvclb-uhfffaoysa-n

Language

English

Type

Text; Journal Article

In AGRIS since: 2024-02-28

Format: MODS

Data Provider

This bibliographic record has been provided by National Agricultural Library

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Links

DOI http://dx.doi.org/10.1016/j.tet.2014.09.074

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