Purification and Characterization Sucrose phosphorylase in Leuconostoc mesenteroides NRRL B-1149
Lee, J.H.;Park, J.S.;Park, H.J.;Cho, J.Y.;Kim, D.M.(Chonnam National University, Gwangju, Republic of Korea)E-mail:dmkim@chonnam.ac.kr | Choi, J.S.(Dongshin University, Naju, Republic of Korea)
Leuconostoc mesenteroides NRRL B-1149 produces various glucosyltransferases for the synthesis of dextran, levan and glucose-1-phosphate using sucrose as a substrate. A sucrose phosphorylase (1149SPase) was purified from L. mesenteroides NRRL B-1149 culture by using hollow fiber filtration (30 kDa cut off), Toyopearl DEAE 650 M column chromatography and following two times of DEAE-Sepharose column chromatographies. The specific activity of the purified 1149SPase was 25.7 (U/mg) with 16% yield.
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