Purification and Characterization Sucrose phosphorylase in Leuconostoc mesenteroides NRRL B-1149

Lee, J.H.;Park, J.S.;Park, H.J.;Cho, J.Y.;Kim, D.M.E-mail:dmkim@chonnam.ac.kr; Choi, J.S.

Purification and Characterization Sucrose phosphorylase in Leuconostoc mesenteroides NRRL B-1149

Lee, J.H.;Park, J.S.;Park, H.J.;Cho, J.Y.;Kim, D.M.(Chonnam National University, Gwangju, Republic of Korea)E-mail:dmkim@chonnam.ac.kr | Choi, J.S.(Dongshin University, Naju, Republic of Korea)

Leuconostoc mesenteroides NRRL B-1149 produces various glucosyltransferases for the synthesis of dextran, levan and glucose-1-phosphate using sucrose as a substrate. A sucrose phosphorylase (1149SPase) was purified from L. mesenteroides NRRL B-1149 culture by using hollow fiber filtration (30 kDa cut off), Toyopearl DEAE 650 M column chromatography and following two times of DEAE-Sepharose column chromatographies. The specific activity of the purified 1149SPase was 25.7 (U/mg) with 16% yield.

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Ключевые слова АГРОВОК

fosfopirolasa leuconostoc mesenteroides phosphorylase purification saccharose sucrosa sucrose 분리

Библиографическая информация

Опубликовано в

Korean Journal of Biotechnology and Bioengineering

Том 19 Выпуск 5 ISSN 1225-7117

Нумерация страниц

pp. 363-367

Другие темы

Disproportionation; Sucrose phosphorylase; Crystal; Acceptor reaction; Purificacion; Nrrl b-1149

Язык

Примечание

Summary(En)

3 tables

4ill., 29 ref.

Переведенный заголовок

Leuconostoc mesenteroides NRRL B-1149의 Sucrose phosphorylase의 분리와 특성 연구

Тип

AGRIS — международная информационная система по сельскохозяйственным наукам и технологиям

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Purification and Characterization Sucrose phosphorylase in Leuconostoc mesenteroides NRRL B-1149

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Библиографическая информация