Anther culture response of different salt tolerance genotypes in bread wheat

\{The present study aimed to investigate the anther ture response of different salt tolerance genotypes in bread wheat well as to determine the optimum hormone balance for anther culture. Four Egyptian bread wheat cultivars, i.e., Gemeiza 7, Gemeiza 9, Sakha 8 and Sakha 93 were selected on the basis of salt tolerance differences in addition to four hybrids between them were used. Salt tolerant variety Sakha 8 Possessed higher response than susceptible variety Gemeiza7, meanwhile varieties possessed intermediate response to anther culture. The hybrid Gem. 7X Sak. 8 showed higher frequency of anther culture response than the other hybrids. Medium I (P4+1.5mg/L 2.4.1) +0.5mg/L BAP) considered as a better hormone balance than the other media in the varieties, in contrast medium III (P4+1.5/L NAA+ 0.5mg/L Kin) gave higher frequency of anther culture response than other media for hybrids. Highly difference of interaction between genotypes X media was und. Highly significant differences were reported for anther Lure response between Sakha 8 X Medium I, as well as hybrid m. 7X Sak. 8) X medium 111. In general, the mean values for varieties and hybrids of seed germination percentage and Plant height under salinity applications ere lower than under normal conditions. Number of tillers consider as a good indicator for screening of tolerant genotypes, as well as Sakha 8 followed Sakha 93 consider as a good donor for salt tolerance genes to other genotypes.The regression coeffient between anther culture response and number of tillers were 7.958 and 41.269, Correlation coeffient between anther culture response and percentage of seed germination were 0.038 and 0.53 under normal and salinity condition, respectively. These findings may be suggest that salt stress condition increase of anther culture response as well as, salt tolerant genotypes (Sakha 8) possessed higher response of anther culture, callus induction and plant regeneration. In conclusion, the above relationships require further intensive researches to insure that idea.